LB Broth / LB Agar

LB Broth, also known as, LB medium, Lysogeny broth, Luria broth, or Luria-Bertani medium, is a commonly used nutritionally rich medium for culturing bacteria. First described in 1951 by Giuseppe Bertani, a 1-liter medium consists of 10 grams of tryptone, 5 grams of yeast extract, and 10 grams of sodium chloride. For salt-sensitive applications, the Lennox modification only includes 5 grams of salt per liter. LB medium and its solidified counterpart, LB agar, are widely-used for growing recombinant E. coli for use in plasmid purification and protein expression. For convenience, commercial LB powder and liquid formulations are available, already mixed and ready-to-use.