In situ hybridization (ISH) blockers are reagents for minimizing non-specific binding and unwanted background signals. Examples include DNA from calf thymus, salmon sperm, and herring sperm, which are used in pre-hybridization and hybridization solutions. These DNA solutions block the non-specific binding of ISH probes to surfaces. Endogenous peroxidases and phosphatases may interfere with the enzyme-substrate-based detection methods, such as HRP and alkaline phosphatase. Ready-to-use blocking solutions are also available for this purpose. Visit the supplier pages for more information, including details on the blocking target.
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Novus Biologicals, a Bio-Techne Brand
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