Cloning vectors act as vehicles that can accept recombinant DNA for reproducing a large number of copies and insertion into new cells. The use of cloning vectors in molecular biology enables the editing, transfer, storage, and study of virtually any gene of interest. There are many types of vector backbones that can be used in cloning, such as yeast, adeno-associated viruses (AAV), plasmids, bacteriophages, cosmids, bacterial artificial chromosomes (BACs), and yeast artificial chromosomes (YACs). Vector choice can be determined by a number of factors, including the expression system, size of the DNA insert, selectable markers, restriction sites, as well as the desired copy number. Some cloning vectors may act as expression vectors as well, containing promoter regions and ribosome binding sites. When choosing, ensure that the gene of interest, cloning vector, downstream expression system are all compatible. Visit the supplier to see more details, such as vector maps and sequences.
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