PCR Nucleotides and dNTP Mixes

In PCR applications, dNTPs (deoxyribonucleotide triphosphates) are a defining component that must be included in each reaction. dNTPs are comprised of nucleotides bound to three phosphate groups and include dATP, dTTP, dCTP and dGTP. In standard PCR applications, each of the four dNTPs are combined in equal amounts, generally at 200 micromolar concentrations when using Taq polymerase. Lowering the concentration can increase the fidelity of polymerization but may reduce yield. Nucleotides can be purchased individually as well in premixed dNTP formats for convenience. Find more information on dNTPs by visiting the provided supplier pages.