Recombinant SARS-CoV-2, S1 Subunit Protein (RBD) from RayBiotech

Product Specs
ItemRecombinant SARS-CoV-2, S1 Subunit Protein (RBD)
CompanyRayBiotech
Price $322.00Supplier Page View Company Product Page
Catalog Number230-30162
Quantity100 µg, 500 µg, 1000 µg
Research AreaAntibody Drug Development, COVID-19, Infectious Disease, Receptor-Ligand Binding
ProteinSpike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)
SpeciesSARS-CoV-2
Molecular WeightRecombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below
FormatLiquid
ConcentrationLot specific (see the label on the vial), determined by BCA protein assay
Source/Expression SystemHEK293 cells
Purity>95 %
Conjugate/TagC-terminal his-tag
ApplicationsWB, ELISA

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RayBiotech

3607 Parkway Lane, Ste. 200

Peachtree Corners, Georgia 30092

United States

Phone: 770-729-2992
888-494-8555

Company Profile

Website: www.RayBiotech.com

(1) In Vivo Iterative Adjuvant Screening Identifies an Intranasal Vaccine Formulation for Elicitation of Protective Mucosal Immune Responses Against SARS-CoV-2PharmaceuticsNovember 3, 2025Yang Jiao, Sara H. Mahmoud, Chengjin Ye, Yuan Luo, Wei-Chiao Huang, Qinzhe Li, Shiqi Zhou, Yiting Song, Moriya Tsuji, Luis Martinez-Sobrido, et al.strain, expressed in human embryonic kidney 293 cells (HEK293), was obtained from Raybiotech (Cat# 230-30162, Peachtree Corners, GA, USA). Proteins were dialyzed into phosphate-buffered saline (PBS) to remove

(2) Integrated antibody language model accelerates IgG screening and design for broad-spectrum antiviral therapyiScienceOctober 17, 2025Hannah F. Almubarak, Wuwei Tan, Andrew D. Hoffmann, Yuanfei Sun, Juncheng Wei, Lamiaa El-Shennawy, Joshua R. Squires, Nurmaa K. Dashzeveg, Brooke Simonton, Yuzhi Jia, et al.EasySep B kit (Stemcell Technologies, cat no 17954). SARS-nCoV-2 Spike RBD (Raybiotech, catalog no. 230–30162) was biotinylated via a commercially available kit (Thermo Sceintific cat no. 21330) and the resulting

(3) T-cell-derived IFN-γ suppresses T follicular helper cell differentiation and antibody responsesThe EMBO JournalApril 1, 2025Eleonora Sala, Maria Nelli, Chiara Laura, Pietro Di Lucia, Cristian Gabriel Beccaria, Elisa B Bono, Marta Mangione, Davide Marotta, Valentina Sperto, Marta Grillo, et al.ELISA was carried out by coating plates with recombinant SARS-CoV-2 S1 subunit protein (RayBiotech, 230-30162) at a concentration of 2 μg/ml in PBS and incubated overnight (O/N) at 4 °C. Subsequently, the plates

(4) Novel antibody language model accelerates IgG screening and design for broad-spectrum antiviral therapybioRxiv : the preprint server for biologyAugust 20, 2024Almubarak HF, Tan W, Hoffmann AD, Sun Y, Wei J, El-Shennawy L, Squires JR, Dashzeveg NK, Simonton B, Jia Y, et al.EasySep B kit (Stemcell Technologies, cat no 17954). SARS-nCoV-2 Spike RBD (Raybiotech, catalog no. 230–30162) was biotinylated via a commercially available kit (Thermo Sceintific cat no. 21330) and the resulting

(5) A Paper-Based Multiplexed Serological Test to Monitor Immunity against SARS-COV-2 Using Machine LearningACS nanoJuly 2, 2024Eryilmaz M, Goncharov A, Han GR, Joung HA, Ballard ZS, Ghosh R, Zhang Y, Di Carlo D, Ozcan A.(N-protein) (230–30194), and two distinct receptors binding domain (RBD) proteins, named RBD-1 (Cat. No 230–30162) and RBD-2 (Cat. No 230–30193), throughout this manuscript, were purchased from RayBiotech Inc. These

(6) N-glycosylation of the SARS-CoV-2 spike protein at Asn331 and Asn343 is involved in spike-ACE2 binding, virus entry, and regulation of IL-6Microbiology and immunologyMay 1, 2024Das T, Luo S, Tang H, Fang J, Mao Y, Yen HH, Dash S, Shajahan A, Pepi L, Huang S, et al.Region,Expression System,Source "S1 Subunit, RBD",SARS-CoV-2,QHD43416,Arg319-Phe541,HEK293,RayBiotech (cat. 230–30162) "S1 Subunit, RBD",SARS-CoV-2,QHD43416,Arg319-Phe541,E. coli,RayBiotech (cat. 230–01102) S2 Subunit,SARS-CoV-2,QHD43416,Met697-Pro1213,HEK293,RayBiotech

(7) Antibody-independent protection against heterologous SARS-CoV-2 challenge conferred by prior infection or vaccinationNature immunologyApril 1, 2024Fumagalli V, Ravà M, Marotta D, Di Lucia P, Bono EB, Giustini L, De Leo F, Casalgrandi M, Monteleone E, Mouro V, et al.ELISA plates were functionalized by coating with recombinant SARS-CoV-2 S1 subunit protein (RayBiotech, 230-30162) at a concentration of 2 μg ml−1 and incubated overnight (O/N) at 4 °C. Subsequently, the plates were

(8) SARS-CoV-2 Protein Nanoparticle Vaccines Formed In Situ From Lyophilized LipidsSmall (Weinheim an der Bergstrasse, Germany)March 1, 2024Jiao Y, Huang WC, Chiem K, Song Y, Sun J, Chothe SK, Zhou S, Luo Y, Mabrouk MT, Ortega J, et al.

(9) Contralateral second dose improves antibody responses to a 2-dose mRNA vaccination regimenThe Journal of clinical investigationJanuary 16, 2024Fazli S, Thomas A, Estrada AE, Ross HA, Xthona Lee D, Kazmierczak S, Slifka MK, Montefiori D, Messer WB, Curlin ME.

(10) Oral Immunization with rVSV Bivalent Vaccine Elicits Protective Immune Responses, Including ADCC, against Both SARS-CoV-2 and Influenza A VirusesVaccinesAugust 23, 2023Ouyang MJ, Ao Z, Olukitibi TA, Lawrynuik P, Shieh C, Kung SKP, Fowke KR, Kobasa D, Yao X.Healthcare, Cat#NA931, Chicago, IL, USA). Recombinant proteins included RBD peptide (RayBiotech, Cat# 230-30162, Peachtree Corners, GA, USA), S1 subunit (RayBiotech, Cat# 230-01101), and S2 subunit (RayBiotech, Cat#

(11) Applications of the Microscale Thermophoresis Binding Assay in COVID-19 ResearchVirusesJune 25, 2023Nydegger DT, Pujol-Giménez J, Kandasamy P, Vogt B, Hediger MA.

(12) Analysis of Protein Glycosylation after Rapid Digestion Using Protease-Containing Membranes in Spin ColumnsJournal of the American Society for Mass SpectrometryJune 7, 2023Cao W, Bruening ML.

(13) Nirmatrelvir treatment of SARS-CoV-2-infected mice blunts antiviral adaptive immune responsesEMBO molecular medicineMay 8, 2023Fumagalli V, Di Lucia P, Ravà M, Marotta D, Bono E, Grassi S, Donnici L, Cannalire R, Stefanelli I, Ferraro A, et al.plates were functionalized by coating with recombinant Sars‐CoV‐2 S1 subunit protein (RayBiotech, #230‐30162) at a concentration of 2 μg/ml and incubated overnight (O/N) at 4°C. Subsequently, the plates were blocked

(14) The SpACE-CCM: A facile and versatile cell culture medium-based biosensor for detection of SARS-CoV-2 spike-ACE2 interactionBiosensors & bioelectronicsMay 1, 2023Ham Y, Cho NC, Kim D, Kim JH, Jo MJ, Jeong MS, Pak BY, Lee S, Lee MK, Chi SW, et al.LCB4, synthesized by GenScript) and the recombinant spike RBD (aa 319–541, Raybiotech, GA, USA, Cat.# 230–30162) or ACE2 (aa 18–740, Raybiotech, Cat.# 230–30165) proteins. 2.7 Long-term storage evaluation of the

(15) Humoral and cellular responses to repeated COVID-19 exposure in multiple sclerosis patients receiving B-cell depleting therapies: a single-center, one-year, prospective studyFrontiers in immunologyJanuary 1, 2023Alfonso-Dunn R, Lin J, Lei J, Liu J, Roche M, De Oliveira A, Raisingani A, Kumar A, Kirschner V, Feuer G, et al.enzyme-linked immunosorbent assay (ELISA) as previously described (57). Recombinant ancestral-based CoV-2 RBD protein (Raybiotech, #230-30162-100) was diluted to a final concentration of 0.5 µg/mL, coated onto 96-well, high-binding surface EIA/RIA

(16) Uptake of severe acute respiratory syndrome coronavirus 2 spike protein mediated by angiotensin converting enzyme 2 and ganglioside in human cerebrovascular cellsFrontiers in neuroscienceJanuary 1, 2023McQuaid C, Solorzano A, Dickerson I, Deane R.

(17) Performance estimation of two in-house ELISA assays for COVID-19 surveillance through the combined detection of anti-SARS-CoV-2 IgA, IgM, and IgG immunoglobulin isotypesPloS oneJanuary 1, 2023Ramírez-Reveco A, Velásquez G, Aros C, Navarrete G, Villarroel-Espíndola F, Navarrete M, Fica A, Plaza A, Castro N, Verdugo C, et al.6-His tag) of Host Cell Receptor Binding Domain (RBD) from HEK293 cells (Raybiotech, Catalog Number: 230–30162) (accession # QHD43416.1). For coating step, a final volume of 50 μl of 20 ng Np or RBD proteins [29]

(18) Guest edited collection serological study of SARS-CoV-2 antibodies in japanese cats using protein-A/G-based ELISABMC veterinary researchDecember 21, 2022Imanishi I, Asahina R, Hayashi S, Uchiyama J, Hisasue M, Yamasaki M, Murata Y, Morikawa S, Mizutani T, Sakaguchi M.

(19) A Recombinant VSV-Based Bivalent Vaccine Effectively Protects against Both SARS-CoV-2 and Influenza A Virus InfectionJournal of virologySeptember 28, 2022Ao Z, Ouyang MJ, Olukitibi TA, Warner B, Vendramelli R, Truong T, Meilleur C, Zhang M, Kung S, Fowke KR, et al.#MBAF2348, EMD Millipore Corp.). Recombinant proteins included SARS-CoV-2 RBD peptides (RayBiotech, Cat. #230-30162) and S1 peptide pool (JPT Peptide Technologies. Cat. #PM-WCPV-S-SU1-1). Influenza M2e peptide, mouse-adapted

(20) A dual-antigen self-amplifying RNA SARS-CoV-2 vaccine induces potent humoral and cellular immune responses and protects against SARS-CoV-2 variants through T cell-mediated immunityMolecular therapy : the journal of the American Society of Gene TherapySeptember 7, 2022McCafferty S, Haque AKMA, Vandierendonck A, Weidensee B, Plovyt M, Stuchlíková M, François N, Valembois S, Heyndrickx L, Michiels J, et al.coated overnight at 4°C with 1 μg/mL recombinant SARS-CoV-2 S1 subunit protein RBD (rSARS-CoV-2 S-RBD, #230-30162-500, RayBiotech) or 500 ng/mL recombinant SARS-CoV-2 N protein (rSARS-CoV-2 N, #230-30164-500, RayBiotech).

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Recombinant SARS-CoV-2, S1 Subunit Protein (RBD) from RayBiotech

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Recombinant SARS-CoV-2, S1 Subunit Protein (RBD) from RayBiotech

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