Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to FA has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated FA and incubated. A competitive inhibition reaction is launched between FA (Standards or samples) and HRP-conjugated FA with the pre-coated antibody specific for FA. The more amount of FA in samples, the less antibody bound by HRP-conjugated FA. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of FA in the sample. The color development is stopped and the intensity of the color is measured