Description
The human LBP kit has been developed for the quantitative measurement of natural and recombinant human LBP in serum, plasma and culture medium. The human LBP Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). Monoclonal antibody specific for human LBP is used for coating (pre-coated and blocked modules). In the first step, the plate will be incubated with the antigen (standard or sample). During this incubation, human LBP is captured by solid bound antibody. Unbound material present in the sample is removed by washing. Now the plate will be incubated with a POD-labelled antibody specific for human LBP (second incubation). Detection step includes TMB as chromogen. The enzyme reaction is stopped by the addition of Stop solution and the absorption at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorptions versus the corresponding concentrations of the known standards. The human LBP concentration of samples with unknown concentrations, which are run concurrently with the standards, can be determined from the standard curve