Description
The Eagle Biosciences IGFBP-3 ELISA is a so-called Sandwich-Assay. It utilizes two specific antibodies of high affinity. First the IGFBP-3 in the sample binds to the immobilized antibody on the microtiter plate. In the following step, the complex of biotinylated anti-IGFBP-3-Antibody and Streptavidin-Peroxidase binds in turn to the immobilised IGFBP-3. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the IGFBP-3 content of the sample. The reaction is stopped by the addition of stop solution and color intensity is quantified by measuring the absorption