Description
Myelin-specific proteins have been purified from bovine brain and used for coating of microtiterplates: Myelin-oligodendrocyticglycoprotein (MOG); Myelin Basic Protein (MBP); Myelin-associated Glycoprotein (MAG); Proteolipoprotein (PLP); Alpha-B-Crystallin (CRY). The antibodies in the samples bind specifically to the immobilized Antigenss. IgG- or IgM specific biotinylated antibodies together with a streptavidin peroxidase conjugate are employed for the detection of the bound antibodies of the sample. A chemical reaction catalyzed by the peroxidase results in a colour change of the substrate, which is stopped after defined period of time. The colour intensity correlates positive with the amount of specific antibodies bound. To assess the quantity in each run a ready-to-use diluted human serum is used for calibration. The amount of specific antibody in the standard has been determined by titration. Thus, this assay quantifies the specific antibodies in the sample in arbitrary Mediagnost Units