Fig 1: (A) WNT3a, (B) ß-catenin, (C) c-Myc, and (D) cyclin D1 levels. The absolute control values of WNT3, ß-catenin, c-Myc, and cyclin D1 protein content were 11.31 ± 0.21 ng/g, 22.14 ± 0.34 ng/g, 14.1 ± 0.52 ng/g, and 8.2 ± 0.31 ng/g, respectively. Results show the mean (n = 9) ± SEM of three independent experimental conditions from cells of different cultures, each developed in triplicate. There were no significant differences between controls treated with and without a vehicle, cells transfected without siRNA or with scramble siRNA, or cells transfected with or without siRNA and treated with a vehicle in any of the targets studied. The data shown in the figures represent control treated with vehicle and transfected without siRNA as 100%. One-way (treatment-response comparisons) and two-way (transfection/treatment comparisons) ANOVA analyses followed by Tukey post-hoc test were developed to determine statistically significant differences between treatments. *** p = 0.001, significantly different from controls; &&& p = 0.001 contrasted with BPA exposure.
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