Fig 1: A) to H) Gene expression during induction of osteogenic in vitro differentiation and inhibition of signaling pathways (Wnt/β-catenin, TGF-βRI, MEK, p38 MAPK, and FGFR/VEGFR) of PDLSCs (RUNX2, SP7, OPG, RANKL, PAR1, POSTN, OC, and COL I) for 2, 7, and 14 days. Calibrating sample: undifferentiated PDLSCs (P4). Housekeeping gene: GAPDH.
Fig 2: A) Gene expression during induction of in vitro osteogenic differentiation and inhibition of signaling pathways (Wnt/β-catenin, TGF-βRI, MEK, p38 MAPK, and FGFR/VEGFR) of PDLSCs (ALP) for 2, 7, and 14 days. Calibrating sample: undifferentiated PDLSCs (P4). Housekeeping gene: GAPDH. B) to G) Protein levels (ELISA) during induction of in vitro osteogenic differentiation and inhibition of signaling pathways (Wnt/β-catenin, TGF-βRI, MEK, p38 MAPK, and FGFR/VEGFR) of PDLSCs (OPG, RANKL, PAR1, POSTN, OC, and COL I) for 2, 7 and 14 days.
Fig 3: A) to I) Gene expression during induction of osteogenic differentiation in vitro of PDLSCs and cell sheet (RUNX2, SP7, OPG, RANKL, PAR1, POSTN, OC, COL I, and ALP) for 2, 7, and 14 days. Calibrating sample: undifferentiated PDLSCs (P4). Housekeeping gene: GAPDH. Gray bar indicates osteogenic and black bar indicates osteogenic + PAR1 medium group.
Fig 4: A) to F) Protein levels (ELISA) during induction of osteogenic differentiation in vitro of PDLSCs and cell sheet (OPG, RANKL, PAR1, POSTN, and OC) for 2, 7, and 14 days.
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