Fig 1: Effect of administration of crude extract, zygo-albuside A, or rutin on testicular expression of (A) p53, (B) miRNA-29a, mRNA and testicular levels of (C) Bax and (D) Bcl-2 in mice with MTX-induced testicular injury. Statistical analysis was performed using ANOVA followed by Tukey’s post hoc test. Values are expressed as mean ± SD (n = 8). A statistically significant difference was assumed at p < 0.01 and denoted by * vs. NC; # vs. MTX.
Fig 2: Effect of M. alysson L. methanolic extract, its polyphenolic fraction, kaempferol, quercetin and rutin on testicular injury-induced mice. (A) Testicular p53 mRNA expression, (B) Testicular Bax level, (C) Testicular Bcl-2 level and (D) Testicular miRNA-29a expression. Data are presented as mean ± SD, (n = 8). * Significant versus normal control. # Significant versus MTX. ^ Significant versus methanolic extract. $ Significant versus polyphenolic fraction. p value = 0.01.
Fig 3: Effects of C. endivia L. crude extract, phenolic fraction, and its isolated compounds (coumaric, vanillic, and ferulic acids) on the testicular apoptosis biomarkers in the experimental mice. (A) p53 expression, (B) Bax levels, (C) Bcl-2 levels, and (D) miR-29a expression. Data are expressed as the mean ± SD. Data were analyzed using one-way ANOVA followed by Bonferroni’s post hoc test. * Significant against normal control group, # significant against MTX control group. Values were considered different significantly at p < 0.05. Bax: Bcl-2-associated X protein, Bcl-2: B-cell lymphoma.
Fig 4: Effect of Met and EGCG on levels of p53 (ng/g tissue; a), Bax (ng/g tissue; b), caspase 9 (ng/g tissue; c), caspase 3 (ng/g tissue; d), and Bcl-2 (ng/g tissue; e) in the brains of all experimental groups. Data are shown as mean ± SEM (n = 6). A single asterisk and a single ampersand (*, &) indicate significance at P < 0.05, and triple asterisks and triple ampersands (***, &&&) indicate significance at P < 0.001. A single asterisk and triple asterisks (*, ***) indicate comparisons with respect to the control group. A single ampersand and triple ampersands (&, &&&) indicate comparisons with respect to the methionine group. Cont, control; EGCG, epigallocatechin-3-gallate; Met, methionine; Met + EGCG, methionine + epigallocatechin-3-gallate
Supplier Page from MyBioSource.com for Mouse Apoptosis regulator BAX ELISA Kit