MojoSort™ Mouse CD3 T Cell Isolation Kit 480031 from BioLegend

Product Specs
ItemMojoSort™ Mouse CD3 T Cell Isolation Kit
CompanyBioLegend
Price $654.00Supplier Page View Company Product Page
Catalog Number480031
Quantity200 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD3
Cell Type (standardized)CD3+ T Cells

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(41) Targeting CXCL16 and STAT1 augments immune checkpoint blockade therapy in triple-negative breast cancerNature communicationsApril 13, 2023Palakurthi B, Fross SR, Guldner IH, Aleksandrovic E, Liu X, Martino AK, Wang Q, Neff RA, Golomb SM, Lewis C, et al.

(42) FGL2-targeting T cells exhibit antitumor effects on glioblastoma and recruit tumor-specific brain-resident memory T cellsNature communicationsFebruary 10, 2023Zhao Q, Hu J, Kong L, Jiang S, Tian X, Wang J, Hashizume R, Jia Z, Fowlkes NW, Yan J, et al.lymph nodes of Balb/c, C57BL/6, FGL2−/−, and LAG3−/− mice and purified with a Mouse CD3 T cell Isolation Kit (480031; BioLegend). Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque PLUS

(43) PD-1 and TIM-3 differentially regulate subsets of mouse IL-17A-producing γδ T cellsThe Journal of experimental medicineFebruary 6, 2023Edwards SC, Hedley A, Hoevenaar WHM, Wiesheu R, Glauner T, Kilbey A, Shaw R, Boufea K, Batada N, Hatano S, et al.background were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (#480031; Biolegend) according to the manufacturer’s instructions. γδ T cell isolation was performed by TCRγδ

(44) Distinct Genetically Determined Origins of Myd88/BCL2-Driven Aggressive Lymphoma Rationalize Targeted Therapeutic Intervention StrategiesBlood cancer discoveryJanuary 6, 2023Flümann R, Hansen J, Pelzer BW, Nieper P, Lohmann T, Kisis I, Riet T, Kohlhas V, Nguyen PH, Peifer M, et al.isolated from spleens of C57BL/6 wild-type mice using the Mouse CD3 T cell isolation kit (BioLegend; cat. #480031), cultured and activated as previously described (27). In brief, T cells were activated in vitro by

(45) TRAM deletion attenuates monocyte exhaustion and alleviates sepsis severityFrontiers in immunologyJanuary 1, 2023Wang J, Wu Y, Lin R, Zhang Y, Li L.coculture Mouse spleen CD3+ T cells were isolates by using MojoSort Mouse CD3 T Cell Isolation Kit (Biolegend, #480031) resulting in >95% purity. Isolated spleen CD3+ T cells were labeled with 5 μM carboxyfluorescein succinimidyl

(46) Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MSSTAR protocolsSeptember 16, 2022Li X, Wu P, Zeng X, Lang Q, Lin Y, Huang H, Qian P.assays,Critical commercial assays,Critical commercial assays ,, Mouse CD3 cell isolation Kit,BioLegend,Cat.480031 Pan T-Cell Isolation Kit,Miltenyi Biotec,Cat.130-0950139 E.Z.N.A.® Stool DNA Kit (200 preps),Omega,Cat#D4015-02 Phusion®

(47) Protein phosphatase 2A catalytic subunit β suppresses PMA/ionomycin-induced T-cell activation by negatively regulating PI3K/Akt signalingThe FEBS journalAugust 1, 2022Gao R, Li X, Gao H, Zhao K, Liu X, Liu J, Wang Q, Zhu Y, Chen H, Xiang S, et al.

(48) Inhibition of D-2HG leads to upregulation of a proinflammatory gene signature in a novel HLA-A2/HLA-DR1 transgenic mouse model of IDH1R132H-expressing gliomaJournal for immunotherapy of cancerMay 1, 2022Chuntova P, Yamamichi A, Chen T, Narayanaswamy R, Ronseaux S, Hudson C, Tron AE, Hyer ML, Montoya M, Mende AL, et al.10-week-old dTG naïve mice were collected using the Mouse CD3 T-cell Negative Selection Kit (BioLegend, #480031), labeled with 2 µM CFSE, washed, and added to all the wells of a round-bottom 96-well plate at 5×105

(49) A microfluidics-based method for isolation and visualization of cells based on receptor-ligand interactionsPloS oneJanuary 1, 2022Dao L, Zhao Q, Hu J, Xia X, Yang Q, Li S.(Jackson, cat. No. 000664) Reagents MojoSort Mouse CD3 T cell Isolation Kit (BioLegend, cat. no. 480023)CellTracker Green BODIPY dye (Thermo Fisher Scientific, cat. no. C2102)CellTracker Red CMTPX dye (Thermo Fisher Scientific

(50) CD146 bound to LCK promotes T cell receptor signaling and antitumor immune responses in miceThe Journal of clinical investigationNovember 1, 2021Duan H, Jing L, Jiang X, Ma Y, Wang D, Xiang J, Chen X, Wu Z, Yan H, Jia J, et al.

(51) Neuronal CD200 Signaling Is Protective in the Acute Phase of Ischemic StrokeStrokeOctober 1, 2021Al Mamun A, Ngwa C, Qi S, Honarpisheh P, Datar S, Sharmeen R, Xu Y, McCullough LD, Liu F.C57BL/6 mouse using the MojoSort™ Mouse CD3 T Cell Isolation Kit according to manufacture instruction (# 480024 BioLegend). The purity of isolated CD3+ cells was confirmed by flow cytometry. The isolated cells were

(52) Hypoxia-Driven Oncometabolite L-2HG Maintains Stemness-Differentiation Balance and Facilitates Immune Evasion in Pancreatic CancerCancer researchAugust 1, 2021Gupta VK, Sharma NS, Durden B, Garrido VT, Kesh K, Edwards D, Wang D, Myer C, Mateo-Victoriano B, Kollala SS, et al.T cells were isolated from spleen using MojoSort™ Mouse CD3 T Cell Isolation Kit (Biolegend Cat no; 480024) as per the manufacturers protocol. Cells were labeled with CFSE (ThermoFisher cat no: C34554) as per

(53) Synchronous effects of targeted mitochondrial complex I inhibitors on tumor and immune cells abrogate melanoma progressioniScienceJune 25, 2021AbuEid M, McAllister DM, McOlash L, Harwig MC, Cheng G, Drouillard D, Boyle KA, Hardy M, Zielonka J, Johnson BD, et al.commercial assays,Critical commercial assays,Critical commercial assays ,, MOJO sort Kit,Biolegend,Cat# 480024 Dynabeads™ Mouse T-Activator CD3/CD28,Thermofisher Scientific,Cat# 1456D Cell Trace Violet,Thermofisher Scientific

(54) Synergistic effects of AAGL and anti-PD-1 on hepatocellular carcinoma through lymphocyte recruitment to the liverCancer biology & medicineMarch 12, 2021Ye X, Wang X, Yu W, Yang Q, Li Y, Jin Y, Su Y, Song J, Xu B, Sun H.spleens and subjected to FACS analysis as previously described. The splenic lymphocytes and T cells (480024, Biolegend) were isolated 3 h after treatment with AAGL in vitro and subjected to FACS analysis. The

(55) Enterovirus 71 Antagonizes Antiviral Effects of Type III Interferon and Evades the Clearance of Intestinal Intraepithelial LymphocytesFrontiers in microbiologyJanuary 1, 2021Dong Y, Liu J, Lu N, Zhang C.MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, Catalog# 480049) and MojoSort™ Mouse CD3 Cell Isolation Kit (Biolegend, Catalog# 480024) respectively, according to the protocol provided by the manufacturer. Plaque Assays Vero cells were

(56) A Defect in Thymic Tolerance Causes T Cell-Mediated Autoimmunity in a Murine Model of COPA SyndromeJournal of immunology (Baltimore, Md. : 1950)May 1, 2020Deng Z, Law CS, Ho FO, Wang KM, Jones KD, Shin JS, Shum AK.prepared as described above. CD3+ T cells were isolated using MojoSort negative selection kit (Biolegend, #480031). Derived cells were counted and purity of the cells were checked by flow cytometry. 10 million CD3+

(57) SUV39H1 Represses the Expression of Cytotoxic T-Lymphocyte Effector Genes to Promote Colon Tumor Immune EvasionCancer immunology researchMarch 1, 2019Lu C, Yang D, Klement JD, Oh IK, Savage NM, Waller JL, Colby AH, Grinstaff MW, Oberlies NH, Pearce CJ, et al.resuspended in PBS, and CD3+ T cells were purified using the MojoSort mouse CD3 T cell isolation kit (Cat #480031, Biolegend) according to the manufacturer’s instructions. The CD3+ T cell purity was above 90%. For

(58) TRADD regulates perinatal development and adulthood survival in mice lacking RIPK1 and RIPK3Nature communicationsFebruary 11, 2019Dowling JP, Alsabbagh M, Del Casale C, Liu ZG, Zhang J.indicated genotypes by negative selection using MojoSort Mouse CD3 T cell Isolation kit (Biolegend, #480031) and stimulated with 5 μg/mL anti-CD3 and 1 μg/mL anti-CD28 antibodies in cRPMI for 16 h or with 1x

(59) Glutathione de novo synthesis but not recycling process coordinates with glutamine catabolism to control redox homeostasis and directs murine T cell differentiationeLifeSeptember 10, 2018Lian G, Gnanaprakasam JR, Wang T, Wu R, Chen X, Liu L, Shen Y, Yang M, Yang J, Chen Y, et al.kit PK-7200 Vector laboratories Commercial assay or kit MojoSort Mouse naive CD4 T Cell Isolation Kit 480031 Biolegend Chemical compound, drug Diethly Fumerate Sigma Aldrich D95654 Chemical compound, drug N-Acetyl-L-cysteine Sigma-Aldrich A7250 Chemical compound,

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MojoSort™ Mouse CD3 T Cell Isolation Kit from BioLegend

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MojoSort™ Mouse CD3 T Cell Isolation Kit from BioLegend

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