Fig 1: Enteritis Induced α-MSH Secretion Upregulation in the Murine Pituitary Gland. (A) The body weight, (B) DAI, (C) colons, (D) spleens, (E) H&E staining of mice in Vehicle and DS (2%DSS induced colitis) group were recorded. (F) Immunofluorescence analysis was conducted to detect c-Fos expression in the PVH of the hypothalamus in mice, and the positive cells expressing c-Fos were quantified (right). The white dashed line represents the PVH region of the mouse hypothalamus. (G) Transcriptional levels of Pomc, Fshb, Tshb, Prl, Lnb in mouse pituitary gland were examined through RT-qPCR. (H) The protein levels of α-MSH, β-endorphin and ACTH in mouse serum (left) and bone marrow supernatant were detected by ELISA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Vehicle: blank control, DS: 2% DSS induced colitis.
Fig 2: Enteritis Induced α-MSH Secretion Upregulation in the Murine Pituitary Gland. (A) The body weight, (B) DAI, (C) colons, (D) spleens, (E) H&E staining of mice in Vehicle and DS (2%DSS induced colitis) group were recorded. (F) Immunofluorescence analysis was conducted to detect c-Fos expression in the PVH of the hypothalamus in mice, and the positive cells expressing c-Fos were quantified (right). The white dashed line represents the PVH region of the mouse hypothalamus. (G) Transcriptional levels of Pomc, Fshb, Tshb, Prl, Lnb in mouse pituitary gland were examined through RT-qPCR. (H) The protein levels of α-MSH, β-endorphin and ACTH in mouse serum (left) and bone marrow supernatant were detected by ELISA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Vehicle: blank control, DS: 2% DSS induced colitis.
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