Fig 1: Marked cholangiocytes isolated from the liver of induced Alb-CreERT2/Confetti mice phenocopy GFP-positive cholangiocytes(A) Experimental scheme: tamoxifen is administered in vivo subcutaneously to newborn mice. The liver is collected at 8 weeks of age, and EpCAM-positive BECs are isolated and sorted according to the expression of the Confetti markers. Liver organoids are then derived from Confetti-positive (ConfettiPos) or Confetti-negative (ConfettiNeg) BECs.(B) Single values and mean with SEM of ConfettiPos EpCAM-positive cells isolated from the livers of 8-week-old Alb-CreERT2/Confetti mice (n = 12) administered with tamoxifen as newborns. Pos, Confetti-positive EpCAM-positive cells; RFP, red fluorescent protein; YFP/GFP, yellow/green fluorescent protein; CFP, cyan fluorescent protein.(C and D) Single values and mean with SEM of the organoid formation efficiency (C) or the percentage of ConfettiPos organoids (D) of ConfettiNeg (n = 6) or ConfettiPos (n = 6) EpCAM-positive sorted cells at the indicated passages and cultured in EM or DM. Wilcoxon matched-pairs signed rank test (C). Friedman test with Dunn’s multiple comparisons test (D).(E) Heatmap of the expression of DEGs resulting from the comparison of positive (GFP or Confetti) samples with negative (GFP or Confetti) samples. Unsupervised clustering highlights the similarity of GFP (see Figure 3) and Confetti samples on both positive and negative conditions.