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Assay Kit
MojoSort(TM) Mouse CD8 T Cell Isolation Kit
MojoSort(TM) Mouse CD8 T Cell Isolation Kit from BioLegend
(4)
Write a review
Citations:
(29)
Supplier Page
Supplier Page from
BioLegend for
MojoSort(TM) Mouse CD8 T Cell Isolation Kit
Description
MojoSort(TM) Mouse CD8 T Cell Isolation Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
MojoSort(TM) Mouse CD8 T Cell Isolation Kit
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
480007
Quantity
10 tests
Applications
Cell Sep - Neg
Format
Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
Reactivity
Mouse
Detection Target
CD8
Cell Type (standardized)
CD8+ T Cell
Conjugate/Tag/Label
MOJO
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (29)
(1)
Interferon-γ couples CD8+ T cell avidity and differentiation during infection
Nature Communications
October 23, 2023
Lion F. K. Uhl, Han Cai, Sophia L. Oram, Jagdish N. Mahale, Andrew J. MacLean, Julie M. Mazet, Theo Piccirilli, Alexander J. He, Doreen Lau, Tim Elliott,
et al.
12-week-old WT or IFN-γRKO OT-I
mice
, using the MojoSortTM CD8+
T-cell
isolation kit and magnets (
Biolegend
, #
480008
and #
480019
). Isolated
T-cells
were resuspended in complete RPMI (RPMI 1640 [
Gibco
, #21870-076] supplemented
(2)
Vaccine-induced antigen archiving enhances local memory CD8+ T cell responses following an unrelated viral infection
Research Square
September 26, 2023
Beth Tamburini, Thu Doan, Tadg Forward, Erin Lucas, Ira Fleming, Aspen Uecker-Martin, Jay Hesselberth, Thomas Morrison
and P14 CD8 + T cells were isolated using the Mojosort CD8
T cell
isolation kit (
Biolegend
Cat. No.
480008
). After CD8 negative selection, the cells were labeled with VPD or
CFSE
to assess proliferation. For
(3)
The immune-metabolic crosstalk between CD3+C1q+TAM and CD8+T cells associated with relapse-free survival in HCC
Frontiers in Immunology
February 9, 2023
Yanying Yang, Lu Sun, Zhouyi Chen, Weiren Liu, Qiyue Xu, Fangming Liu, Mingyue Ma, Yuwen Chen, Yan Lu, Hao Fang,
et al.
single-cell suspension. CD8+ T cells were purified through negatively magnetic sorting (
Biolegend
, Cat.
480008
), activated with anti-
CD3
(
eBioscience
, Cat.
16-0031-85
) and anti-
CD28
(
eBioscience
, Cat.
16-0281-85
)
(4)
CD59-Regulated Ras Compartmentalization Orchestrates Antitumor T-cell Immunity
Cancer Immunology Research
December 2, 2022
Luying Li, Peipei Ding, Xinyue Lv, Suhong Xie, Ling Li, Jianfeng Chen, Danlei Zhou, Xiaochao Wang, Qi Wang, Wei Zhang,
et al.
mouse
CD4+/CD8+
T-cell
isolation kit (CD4+
T-cell
isolation kit,
480033
; CD8+
T-cell
isolation kit,
480008
;
BioLegend
, San Diego, CA), and the purity was confirmed by FACS. The purities of CD4+ and CD8+ T cells
(5)
A tick C1q protein alters infectivity of the Lyme disease agent by modulating interferon γ
Cell reports
November 22, 2022
Tang X, Arora G, Matias J, Hart T, Cui Y, Fikrig E.
isolated from splenocytes with MojoSort™
mouse
CD4 and
CD8
T cell
isolation kits (
BioLegend
, #
480005
and #
480007
). The purity of the isolated cells was evaluated by
flow cytometry
with the antibodies of
FITC anti-mouse CD3
(6)
CD36-mediated metabolic crosstalk between tumor cells and macrophages affects liver metastasis
Nature communications
October 2, 2022
Yang P, Qin H, Li Y, Xiao A, Zheng E, Zeng H, Su C, Luo X, Lu Q, Liao M,
et al
.
were isolated from
spleen
cells using magnetic bead sorting according to manufacturer’s instructions (#
480008
,
Biolegend
).
Spleen
macrophages
were allowed to adhere for 2 h at 37 °C and the suspension cells were
(7)
Tumorous expression of NAC1 restrains antitumor immunity through the LDHA-mediated immune evasion
Journal for ImmunoTherapy of Cancer
September 23, 2022
Yijie Ren et al.
by the magnetic bead separation using the MojoSort Mouse CD8 T Cell Isolation Kit from
BioLegend
(#
480008
, San Diego, California, USA). Purified CD8+ T cells were activated with anti-CD3 (5 µg/mL, plate-coated)
(8)
Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T-cell suppression
Molecular oncology
January 1, 2022
Itoh G, Takagane K, Fukushi Y, Kuriyama S, Umakoshi M, Goto A, Yanagihara K, Yashiro M, Tanaka M.
cell sorting
CD8+ T cells were purified from C57BL/6JJcl
mouse
spleens using MojoSort isolation kits (
480008
BioLegend
, San Diego, CA, USA). CD8+ T cells (2 × 106) were incubated with CM derived from
fibroblasts
(9)
UTX promotes CD8
+
T cell-mediated antiviral defenses but reduces T cell durability
Cell reports
April 13, 2021
Mitchell JE, Lund MM, Starmer J, Ge K, Magnuson T, Shpargel KB, Whitmire JK.
KAPA mRNA Capture Beads",
Roche
,Cat#
07962193001
MojoSort Mouse CD8 T Cell Isolation Kit,
Biolegend
,Cat#
480008
MojoSort Streptavidin Nanobeads,
Biolegend
,Cat#
480016
UltraComp eBeads
Compensation beads
,
ThermoFisher Scientific
(10)
Targeted Deletion of CXCR2 in Myeloid Cells Alters the Tumor Immune Environment to Improve Antitumor Immunity
Cancer Immunology Research
February 1, 2021
Jinming Yang, Chi Yan, Anna E. Vilgelm, Sheau-Chiann Chen, Gregory D. Ayers, Christopher A. Johnson, Ann Richmond
tumor
samples.
Purification
of tissue immune cells The Mojosort mouse CD8+ T-cell isolation kit (#
480008
,
Biolegend
) was used to negatively isolate CD8+ T-cells per the manufacture’s instruction from digested
(11)
Methotrexate enhances antigen presentation and maturation of tumour antigen-loaded dendritic cells through NLRP3 inflammasome activation: a strategy for dendritic cell-based cancer vaccine
Therapeutic Advances in Medical Oncology
January 21, 2021
Gao-Na Shi, Min Hu, Chengjuan Chen, Junmin Fu, Shuai Shao, Yu Zhou, Lei Wu, Tiantai Zhang
ionomycin (1 µg/mL), Brefeldin A solution (1000×;
420601
), and MojoSort™ Mouse CD8 T Cell Isolation Kit (
480008
) were also purchased from
BioLegend
. Rabbit mAbs against caspase-1 (D7F10; #
3866
), cleaved caspase-1
(12)
Extracellular Vesicles from
Akkermansia muciniphila
Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8
+
T Cells and Macrophages
International journal of nanomedicine
January 1, 2021
Luo ZW, Xia K, Liu YW, Liu JH, Rao SS, Hu XK, Chen CY, Xu R, Wang ZX, Xie H.
humidified atmosphere with 5% CO2. For
mouse
naïve
CD8
+
T Cell
isolation, the commer cial isolation kit (
480008
;
Biolegend
) was used according to the manufacturer’s protocol. Briefly, the spleens of C57BL/6
mice
(13)
Manganese is critical for antitumor immune responses via cGAS-STING and improves the efficacy of clinical immunotherapy
Cell research
November 1, 2020
Lv M, Chen M, Zhang R, Zhang W, Wang C, Zhang Y, Wei X, Guan Y, Liu J, Feng K,
et al
.
spleens of 6–8 weeks old OT-I
mice
were purified by MojoSort™ Mouse CD8 T Cell Isolation Kit (
Biolegend
,
480008
). CD8+ T cells were mixed with BMDC and incubated with pre-plated B16F10-
OVA
-GFP cells (CD8+ T:BMDC:
Tumor
(14)
PPARα Inhibition Overcomes Tumor-Derived Exosomal Lipid-Induced Dendritic Cell Dysfunction
Cell reports
October 20, 2020
Yin X, Zeng W, Wu B, Wang L, Wang Z, Tian H, Wang L, Jiang Y, Clay R, Wei X,
et al
.
(15)
PD-L1 Reverse Signaling in Dermal Dendritic Cells Promotes Dendritic Cell Migration Required for Skin Immunity
Cell reports
October 13, 2020
Lucas ED, Schafer JB, Matsuda J, Kraus M, Burchill MA, Tamburini BAJ.
Assays
,, IP-One Gq kit,Cis-Bio,Cat#62IPAPEB MojoSort Mouse CD8 T Cell Isolation Kit,
Biolegend
,Cat#
480008
F-actin Visualization Biochem Kit (
fluorescence
format),
Cytoskeleton Inc
.,Cat#
BK005
Experimental Models
:
(16)
Autophagy deficiency promotes triple-negative breast cancer resistance to T cell-mediated cytotoxicity by blocking tenascin-C degradation
Nature communications
July 30, 2020
Li ZL, Zhang HL, Huang Y, Huang JH, Sun P, Zhou NN, Chen YH, Mai J, Wang Y, Yu Y,
et al
.
from Sun Yat-sen University
Cancer
Center) using the MojoSortTM Mouse CD8 T Cell Isolation Kit (Cat#
480007
,
Biolegend
) according to the manufacturer’s protocol. Isolated OT-I CD8+ T cells were maintained in
(17)
Self-Assembled Multivalent Aptamer Nanoparticles with Potential CAR-like Characteristics Could Activate T Cells and Inhibit Melanoma Growth
Molecular therapy oncolytics
June 26, 2020
Bai C, Gao S, Hu S, Liu X, Li H, Dong J, Huang A, Zhu L, Zhou P, Li S,
et al
.
nylon hair column (Polyscience, 21759-1) or a
mouse
CD4/CD8
T cell
isolation kit (
Biolegend
,
480005
/
480007
) and added to 1640 medium for subsequent experiments.
T cells
were then stained with CFDA-SE dyes (
Invitrogen
,
(18)
Host cytosolic RNA sensing pathway promotes T Lymphocyte-mediated mycobacterial killing in macrophages
PLoS pathogens
May 1, 2020
Cheng Y, Kiene NJ, Tatarian A, Eix EF, Schorey JS.
Cell Isolation Kit (Cat.
480005
,
Biolegend
) and MojoSort™ and mouse CD8+ T Cell Isolation Kit (Cat.
480007
,
Biolegend
). Purified CD4+ T cells and CD8+ T cells were added to the M.avium-infected BMMs culture
(19)
Genetically stable poliovirus vectors activate dendritic cells and prime antitumor CD8 T cell immunity
Nature communications
January 27, 2020
Mosaheb MM, Dobrikova EY, Brown MC, Yang Y, Cable J, Okada H, Nair SK, Bigner DD, Ashley DM, Gromeier M.
transgenic
mouse
spleen
(Jackson Laboratories #003831) using the
Biolegend
CD8
T cell
isolation kit #
480008
) and 105
GMCSF
-BMDCs (with appropriate treatment) were cocultured for 3 days in a 96-well U-bottom plate.
(20)
USP7 targeting modulates anti-tumor immune response by reprogramming Tumor-associated Macrophages in Lung Cancer
Theranostics
January 1, 2020
Dai X, Lu L, Deng S, Meng J, Wan C, Huang J, Sun Y, Hu Y, Wu B, Wu G,
et al
.
from the spleens of C57BL/6
mice
, following the MojoSort™ Mouse CD8+T Cell Isolation Kit protocol (
480008
,
Biolegend
). M2 MΦs induced by IL-4 and IL-13 were treated with P5091 for 24 h, and the conditioned
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Reviews
Write a Review
(1)
1/10/2020
Good Isolation Kit to Recover Untouched Mouse CD8+ T Cells
Dr. Min Hee Oh
We are very happy in the end using this kit since it's very cheap per isolation. Yield and purity is very acceptable especially with transgenic mouse T cells.
(1)
1/6/2020
Good BioLegend Mouse CD8 T Cells Isolation Kit
Wei Xu
You can use this kit to get acceptable yield and purity to isolate CD8 cells from mouse with reasonable price.
(1)
9/21/2017
Isolation Of Mouse CD8 T Cell (Negative Selection)
Jeeho Lee
The purpose is to isolate pure CD8 T cells (untouched) for adoptive transfer into mice for an in vivo study.
(1)
4/6/2017
MojoSort Mouse CD8 T Cell Isolation Kit Review
Lana Jagannathan
Our lab has generated a mouse model that expresses three transgenic human host factors that allows successful acute HIV infection in these mice. We are currently testing a novel vaccine candidate, and...
Read Review
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MojoSort(TM) Mouse CD8 T Cell Isolation Kit from BioLegend
Supplier Page
View Company Product Page
Pricing Info
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