Maxwell RSC miRNA Tissue Kit from Promega

Product Specs
ItemMaxwell RSC miRNA Tissue Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1460
Quantity48 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA, Small RNA, miRNA
Sample TypeTissue samples

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Impact of Heterogeneous DNA Methylation on the Accuracy of Quantitative Methylation-Specific PCR for Detecting DNA Hypermethylation in Prostate CancerInternational Journal of Molecular SciencesFebruary 27, 2026Wieke C. H. Visser, Hans de Jong, Laureen B. Janssen, Jolly Shrivastava, Peter F. A. Mulders, Jack A. Schalken, Willem J. G. Melchers(Promega Corporation, Madison, WI, USA), with the Maxwell RSC miRNA Plasma and Serum Kit (cat. no. AS1460, Promega Corporation, Madison, WI, USA) with a protocol optimized for urine. First, 10 mL of urine was

(2) miRNA expression profile as a potential tool for discrimination between bacterial and interstitial cystitisFrontiers in ImmunologyFebruary 16, 2026Dominika Peskar, Nika Kojc, Andreja Erman, Emanuela BoštjančičUSA; code AS1440) and the Maxwell® RSC miRNA tissue kit (Promega, Madison, WI 53711-5399, USA; code AS1460). This combination enables the extraction of miRNAs from FFPE tissue samples, as suggested by the manufacturer

(3) Targeting metabolic vulnerabilities: REV-ERB agonist SR9009 potentiates sorafenib efficacy in liver cancerCell Death DiscoveryJanuary 19, 2026Silvia Sabbioni, Paola Guerriero, Ram C. Shankaraiah, Laura Masatti, Angelo Michilli, Cristian Bassi, Lucilla D’Abundo, Farzaneh Moshiri, Bruno De Siena, Edi Simoni, et al.Maxwell RSC instrument (Promega Italia, Milan) and a Maxwell RSC miRNA from a tissue purification kit (#AS1460, Promega) according to the manufacturer’s instructions. Free heme, lipid peroxidation (MDA) and intracellular

(4) Modulation of the miR-485-3p/PGC-1α Pathway by ASO-Loaded Nanoparticles Attenuates ALS PathogenesisInternational Journal of Molecular SciencesJanuary 7, 2026In Soo Ryu, Dae-In Ha, Yeon-Joo Jung, Hyo Jin Lee, Insun Kim, Yu Na Lim, Hyun Su Min, Seung Hyun Kim, Ilsang Yoon, Hyun-Jeong Cho, et al.isolated from HMC3 cells and mouse spinal cords tissues using the Maxwell® RSC miRNA Tissue kit (Cat #AS1460, Promega, Madison, WI, USA) according to the manufacturer’s instructions. The concentration and purity

(5) TBC1D22B Regulates ER‐to‐Golgi Trafficking via RAB1B Inactivation and Promotes Oncogenic Programs in Breast CancerAdvanced ScienceNovember 1, 2025Flavia Martino, Mariadomenica Lupi, Alessandra Murabito, Fabio Bedin, Giulia Villari, Linda Andreoli, Stefano Freddi, Bronislava Matoskova, Rosa Pennisi, Stella Fontana, et al.] Real‐Time PCR Total cellular RNA was extracted from silenced cells using the Maxwell RSC miRNA Tissue kit (AS1460, Promega) with the Maxwell RSC Instrument (AS4500, Promega). cDNA preparation was performed using the

(6) Chromatin remodeling restrains oncogenic functions in prostate cancerNat CommunOctober 16, 2025Valentina Rosti, Giovanni Lembo, Cristiano Petrini, Francesca Gorini, Roberto Quadri, Chiara Cordiglieri, Margherita Mutarelli, Elisa Salviato, Elisabetta Casari, Emanuele Di Patrizio Soldateschi, et al.stabilized in 200 µl of 1Thioglycerol/Homogenization Solution of the Maxwell® RSC miRNA Tissue Kit (Promega, AS1460) and stored frozen at −80 °C for total RNA automated purification using Maxwell® RSC 48 Instrument (Promega,

(7) Transcriptome analysis of HPV16-positive cells expressing intrabodies targeting E6 and E7 oncoproteins to unravel intrabody antitumor activityJ Transl MedOctober 14, 2025Susanna Falcucci, Sabrina Tait, Maria Vincenza Chiantore, Anna Rita Ciccaglione, Carla Amici, Paola Di Bonito, Luisa Accardi(Promega Corporation, Madison, WI, USA), with the purification kit Maxwell RSC miRNA from Tissue (#AS1460, Promega) according to the manufacturer’s instructions. For RT-qPCR analysis, RNA was purified using

(8) Soma-localized Rab39 inhibits synaptic autophagy by controlling trafficking of Atg9 vesiclesThe EMBO JournalAugust 21, 2025Ayse Kilic, Gokhan Ozturan, Dirk Vandekerkhove, Sabine Kuenen, Jef Swerts, Esther Muñoz Pedrazo, Carles Calatayud Aristoy, Abril Escamilla Ayala, Nikky Corthout, Pablo Hernández Varas, et al.SuperScript III First-Strand Synthesis System,Thermo Fisher Thermo Fisher,Cat#: 18080051 Maxwell RSC microRNA tissue kit,Promega,Cat#: AS1460 Software,Software,Software ZEN Blue,Carl Zeiss Microscopy GmbH,version 2.3 lite ZEN Black,Carl Zeiss

(9) Regulation of RPE65 expression in human retinal pigment epithelium cellsSci RepJuly 25, 2025Olga A. Postnikova, Samuel William, Sheetal Uppal, Steven L. Bernstein, Eugenia Poliakov, Igor B. Rogozin, T. Michael RedmondNAM-MEM media (3x), and PBS (3x) before adding 720 µL of homogenization buffer with 2% thioglycerol (AS1460, Promega) to each plate. Cells were scraped with a cell scraper, collected, and stored at −80 °C. Total

(10) Circulating miRNAs as potential non-invasive biomarkers for ANCA-associated glomerulonephritisFront ImmunolJuly 17, 2025Matic Bošnjak, Željka Večerić-Haler, Živa Pipan Tkalec, Jakob Tomšič, Emanuela Boštjančič, Nika Kojc53711-5399, USA; code AS1440) and Maxwell® RSC miRNA tissue kit (Promega, Madison, WI 53711-5399, USA; code AS1460). For total RNA isolation from serum samples, 200 µl of stored serum was processed with miRNeasy Serum/Plasma

(11) Characterization of extracellular vesicles and miRNA released by cerebral organoids☆Current Research in ToxicologyMarch 8, 2025Brian B. Silver, Rick Fannin, Kevin Gerrish, Erik J. Tokarusing the Maxwell automated nucleic acid extraction system with the Maxwell® RSC miRNA Tissue Kit (AS1460, Promega) according to the manufacturer’s protocol. Fragment analysis was performed on a 5200 Fragment

(12) NEAT1-mediated regulation of proteostasis and mRNA localization impacts autophagy dysregulation in Rett syndromeNucleic Acids ResFebruary 8, 2025Edilene Siqueira, Cecilia D Velasco, Ariadna Tarrasón, Marta Soler, Tara Srinivas, Fernando Setién, Cristina Oliveira-Mateos, Marta Casado-Pelaez, Laura Martinez-Verbo, Judith Armstrong, et al.ALDH1L1. Total RNA sequencing Total RNA was extracted with Promega’s Maxwell RSC microRNA (miRNA) Tissue kit (AS1460, Promega, Madison, WI, USA). RNA-seq libraries were generated and sequenced by BGI (Warsaw), in 150-bp

(13) Cell Membrane-Integrated Neuroligin-1 Regulates the Anti-Inflammatory Effects of CRC Cell-Derived ExosomesInt J Mol SciJanuary 9, 2025Mohammad Mahmoudian, Francesco Trotta, Stefania Raimondo, Federico Bussolino, Marco Areseto the manufacturer’s instructions, the total RNA was extracted using Maxwell® RSC miRNA Tissue Kit (AS1460, Promega, Madison, WI, USA). RNA purity was checked using the NanoPhotometer1 spectrophotometer (IMPLEN,

(14) The integrated stress response drives MET oncogene overexpression in cancersThe EMBO JournalJanuary 7, 2025Marina Cerqua, Marco Foiani, Carla Boccaccio, Paolo M Comoglio, Dogus M Altintaspolymerase chain reaction Total RNA was isolated from cells using the Maxwell RSC miRNA Tissue Kit (Catalogue number AS1460, Promega) with the Maxwell RSC Instrument and reverse transcribed using the SuperScript III First-Strand Synthesis System

(15) Air pollution exposure is associated with gene expression in childrenEnvironmental EpigeneticsDecember 24, 2024Siddhartha Das, Amanda Rundblad, Irene Fontes Marques, Ana Goncalves Soares, Vincent W Jaddoe, Martine Vrijheid, Mark Nieuwenhuijsen, Joost Verlouw, Jason Matthews, Kirsten B Holven, et al.−20°C before RNA isolation. The Maxwell® RSC miRNA Tissue kits and Maxwell® RSC instrument (artnr. AS1460 and AS4500, respectively; Promega, Madison, WI, USA) were used for extraction of total RNA, including

(16) DNA demethylation triggers cell free DNA release in colorectal cancer cellsGenome MedicineOctober 9, 2024Valeria Pessei, Marco Macagno, Elisa Mariella, Noemi Congiusta, Vittorio Battaglieri, Paolo Battuello, Marco Viviani, Giulia Gionfriddo, Simona Lamba, Annalisa Lorenzato, et al.[56]. Transcriptomics analysis RNA from cells was extracted using the Maxwell® RSC miRNA Tissue Kit (Promega, Cat# AS1460) and RNA-seq analysis was performed using TruSeq® Stranded mRNA Library Prep (Illumina, cat# 20020594),

(17) Nanodynamo quantifies subcellular RNA dynamics revealing extensive coupling between steps of the RNA life cycleNature CommunicationsSeptember 4, 2024Lucia Coscujuela Tarrero, Valeria Famà, Giacomo D’Andrea, Simone Maestri, Anna de Polo, Stefano Biffo, Mattia Furlan, Mattia PelizzolaThe RNA in the three subcellular fractions was extracted using Maxwell RSC miRNA tissue Kit (Promega AS1460). Total RNA was quantified, and mRNA purification was performed with up to 200ug of Total RNA using

(18) α7 nicotinic receptor activation mitigates herpes simplex virus type 1 infection in microglia cellsAntiviral ResearchAugust 1, 2024Shih-Heng Chen, Joanne C. Damborsky, Belinda C. Wilson, Rick D. Fannin, James M. Ward, Kevin E. Gerrish, Bo He, Negin P. Martin, Jerrel L. Yakelextracted from cultures using the Maxwell RSC instrument and Maxwell miRNA Tissue Kit (Promega, Cat. # AS1460) according to the manufacturer's protocol. Reverse transcription was performed with oligo dT primers.

(19) Transcriptome‐wide gene expression outlier analysis pinpoints therapeutic vulnerabilities in colorectal cancerMolecular OncologyJune 1, 2024Elisa Mariella, Gaia Grasso, Martina Miotto, Kristi Buzo, Nicole Megan Reilly, Pietro Andrei, Pietro Paolo Vitiello, Giovanni Crisafulli, Sabrina Arena, Giuseppe Rospo, et al.obtained for the remaining 86 CRC cell lines. Total RNA was extracted using Maxwell® RSC miRNA Tissue Kit (AS1460; Promega). The quantification of RNA was performed by Thermo Scientific Nanodrop 1000 (Agilent, Santa

(20) Biological and targeting differences between the rare KRAS A146T and canonical KRAS mutants in gastric cancer modelsGastric CancerMay 1, 2024Elisabetta Puliga, Chiara De Bellis, Sandra Vietti Michelina, Tania Capeloa, Cristina Migliore, Claudia Orrù, Gian Luca Baiocchi, Giovanni De Manzoni, Filippo Pietrantonio, Rossella Reddavid, et al.SOS1 gene expression, total RNA was extracted using Maxwell® RSC miRNA from Tissue and Plasma or Serum AS1460 (Promega) and reverse transcribed into complementary DNA using the High-Capacity complementary DNA Reverse

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