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Nucleic Acid Purification
An Overview of RNA Isolation Kits
FFPE RNA Isolation Kits
Maxwell RSC RNA FFPE Kit
Supplier Images
Published Figures
Maxwell RSC RNA FFPE Kit
Maxwell RSC RNA FFPE Kit
Maxwell RSC RNA FFPE Kit from Promega
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Citations:
(60)
Figures:
(3)
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Maxwell RSC RNA FFPE Kit
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Description
The Maxwell RSC RNA FFPE Kit is used with the Maxwell RSC Instrument to provide efficient, automated purification of RNA from mammalian formalin-fixed, paraffin-embedded (FFPE) tissue samples
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC RNA FFPE Kit
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS1440
Quantity
48 preps
Source
Cell/Tissue, Fixed Tissue
Nucleic Acid Type
Total RNA
Sample Type
Formalin-fixed, paraffin-embedded tissue
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (60)
(1)
Divergent myeloid and lymphoid immune landscapes in HPV/p16 positive and HPV/p16 negative oropharyngeal squamous cell carcinomas and their lymph node metastases
Mol Med
April 30, 2026
Raphaela Graessle, Iris Piwonski, Achim Franzen, Heidi Olze, Anja A. Kühl, Michael Hummel, Ulrike Erben, Annekatrin Coordes
10–20 μm FFPE sections using the bead based
Maxwell® RSC RNA FFPE Kit
(
Promega
, Madison, WI, USA; Cat.
AS1440
) according to the manufacturer’s protocol. Tissue sections were scraped, centrifuged, and processed
(2)
ColoStem, a core oncofetal signature that identifies poor prognosis colorectal tumors
British Journal of Cancer
February 20, 2026
Laura Solé, Eric Canton, María Maqueda, Teresa Lobo-Jarne, Antonio Barbáchano, Ferran Torres, Julia-Jié Cabré-Romans, Ángela Montoto, Lierni Fernández-Ibarrondo, Daniel Martínez-Garcia,
et al.
samples (from three wells of 24-well plates), using the Automated
Maxwell RSC RNA FFPE kit
(
Promega
;
AS1440
) for
human
CRC paraffin-embedded samples and using the Plasma/Serum Circulating and Exosomal
RNA Purification Kit
(3)
miRNA expression profile as a potential tool for discrimination between bacterial and interstitial cystitis
Frontiers in Immunology
February 16, 2026
Dominika Peskar, Nika Kojc, Andreja Erman, Emanuela Boštjančič
USA) and a combination of the
Maxwell® RSC RNA FFPE kit
(
Promega
, Madison, WI 53711-5399, USA; code
AS1440
) and the
Maxwell® RSC miRNA tissue kit
(
Promega
, Madison, WI 53711-5399, USA; code
AS1460
). This combination
(4)
Liver-specific loss of Atg9a perturbs lipid metabolism and hepatocyte integrity
Autophagy Reports
December 31, 2025
Elodie Mailler, Asmita Singh, Michal Jarnik, Yan Li, Lynne Holtzclaw, Victoria Hoffmann, Sohtaro Mine, Paulina Stallcup, Laleh Ordoubadinia, Carlos M. Guardia
gene expression analysis
RNA was purified from FFPE sections using the
Maxwell RSC RNA FFPE Kit
(
Promega
,
AS1440
) with the
Maxwell RSC Instrument
, following the manufacturer’s protocol. Briefly, 3–4 sections (~30
(5)
RNA-seq Splicing Profile of the CDH1 Gene and Its Impact on the Clinical Pathogenicity Classification of CDH1 Variants: A Description of Alternative and Pathogenic Splicing Patterns
Cancers (Basel)
October 14, 2025
Molka Sebai, Roseline Tang, Yahia Adnani, Alice Fievet, Odile Cabaret, Marie-Aude Robert de Rancher, Nathalie Auger, Yasmina Elaribi, Houweyda Jilani, Jean-Marc Limacher,
et al.
(
Promega Corporation
, Madison, WI, USA) on
Promega
Maxwell RSC Instrument
for LCLs and
Maxwell RSC RNA FFPE kit
(
AS1440
) for FFPE blocks. RNA yield and RNA quality were checked on TapeStation using RNA ScreenTape (Agilent
(6)
Detecting actionable mutations from matched plasma-based versus tissue next-generation sequencing in advanced non-small cell lung cancer: a retrospective single centre analysis on site
J Exp Clin Cancer Res
August 6, 2025
Christophe Bontoux, Caroline Lacoux, Jonathan Benzaquen, Jacques Boutros, Guylène Rignol, Elodie Long-Mira, Sandra Lassalle, Maryline Allegra, Doriane Bohly, Mathieu Garcia,
et al.
(
Promega
, #
AS4500
) with the
Maxwell RSC FFPE Plus DNA Kit
(#
AS1720
) and
Maxwell RSC RNA FFPE Kit
(#
AS1440
). Extracted nucleic acids were stored at -80°C to preserve integrity.
NGS
was performed using the
Oncomine™ Precision Assay GX
(7)
Comprehensive genetic profiling using tissue and blood in locally advanced tumors
Oncol Rep
August 5, 2025
Kenji Fujiyoshi, Rie Sugihara, Naoki Miyamoto, Yoriko Watanabe, Tomoya Sudo, Sanae Numata, Jun Akiba, Hideyuki Abe, Yuka Ichinose, Kenji Inoue,
et al.
Maxwell RSC FFPE Plus DNA kit
(cat. no.
AS1720
;
Promega
, Corp.) and
Maxwell RSC RNA FFPE kit
(cat. no.
AS1440
;
Promega
, Corp.). Whole blood for plasma analysis was processed within 15 min of collection. To obtain
(8)
P2X7 a new therapeutic target to block vesicle-dependent metastasis in colon carcinoma: Role of the A2A/CD39/CD73 axis
Cell death & disease
August 4, 2025
Pegoraro A, De Marchi E, Ruo L, Zanoni M, Chioccioli S, Caderni G, Alfieri L, Grignolo M, Ulivi P, Passardi A,
et al
.
Total RNA was extracted from
paraffin-embedded tissue
sections using the
Maxwell RSC RNA FFPE Kit
(
AS1440
,
Promega
) on a Maxwell CSC 48 automated nucleic acid extraction system (Promega) according to manufacturer
(9)
Circulating miRNAs as potential non-invasive biomarkers for ANCA-associated glomerulonephritis
Front Immunol
July 17, 2025
Matic Bošnjak, Željka Večerić-Haler, Živa Pipan Tkalec, Jakob Tomšič, Emanuela Boštjančič, Nika Kojc
USA), in combination with the
Maxwell® RSC RNA FFPE kit
(
Promega
, Madison, WI 53711-5399, USA; code
AS1440
) and
Maxwell® RSC miRNA tissue kit
(
Promega
, Madison, WI 53711-5399, USA; code
AS1460
). For total RNA
(10)
Tumor clusters with divergent inflammation and human retroelement expression determine the clinical outcome of patients with serous ovarian cancer
Molecular Oncology
June 10, 2025
Laura Glossner, Markus Eckstein, Christoph Mark, Matthias W. Beckmann, Arndt Hartmann, Pamela L. Strissel, Reiner Strick
treated with methylene blue, and the RNA was isolated using the
Maxwell RSC RNA FFPE kit
(
Promega
, #
AS1440
, Walldorf, Germany). Following DNase I treatment, the RNA was further purified and quantified. A NanoString
(11)
Single-cell multimodal analysis reveals tumor microenvironment predictive of treatment response in non-small cell lung cancer
Sci Adv
May 23, 2025
Yuanning Zheng, Christoph Sadée, Michael Ozawa, Brooke E. Howitt, Olivier Gevaert
performed FFPE
RNA-seq
. Total RNA was isolated using the
Promega
Maxwell RSC RNA FFPE Kit
(catalog no.
AS1440
), and the quality and quantity were measured using the Tapestation 4200 (Agilent Technologies) and Qubit
(12)
Mucosal multivalent NDV-based vaccine provides cross-reactive immune responses against SARS-CoV-2 variants in animal models
Front Immunol
March 17, 2025
Irene González-Domínguez, Adam Abdeljawad, Tsoi Ying Lai, Marta Boza, Stephen McCroskery, Nicholas Lemus, Stefan Slamanig, Gagandeep Singh, Prajakta Warang, Temima Yellin,
et al.
performed using
Maxwell RSC RNA FFPE Kit
protocol following the manufacturer’s instructions (Catalog number
AS1440
,
Promega
) in the Biorepository and Pathology Core (ISMMS). A total of 50 µm scrolls (3 µm thickness
(13)
The Impact of Tartrazine on DNA Methylation, Histone Deacetylation, and Genomic Stability in Human Cell Lines
Nutrients
March 6, 2025
Afshin Zand, John M. Macharia, Istvan Szabó, Gellért Gerencsér, Ádám Molnár, Bence L. Raposa, Timea Varjas
possible by the
Maxwell® RSC Instrument
(
Promega
, Fitchburg, WI, USA) and a
Maxwell® RSC RNA FFPE Kit
(
AS1440
,
Promega
, Fitchburg, WI, USA). Thermo Scientific NanoDropTM 2000 (Thermo Fisher Scientific, Grand Island,
(14)
Transcriptome analysis of wild olive (Olea Europaea L. subsp. europaea var. sylvestris) clone AC18 provides insight into the role of lignin as a constitutive defense mechanism underlying resistance to Verticillium wilt
BMC Plant Biol
March 6, 2025
Beatriz Mascuñano, Jerónimo Coto-Elena, Víctor M. Guerrero-Sánchez, Candelas Paniagua, Rosario Blanco-Portales, José L. Caballero, José L. Trapero-Casas, Rafael M. Jiménez-Díaz, Fernando Pliego-Alfaro, José A. Mercado,
et al.
RSC Instrument (manufactured by Promega Corporation, Wisconsin, USA) and
Maxwell® RSC RNA FFPE kit
(
AS1440
,
Promega
, Wisconsin, USA) were used. The process was conducted according to the manufacturer’s instructions.
(15)
Examining the Formalin Fixation Method for Maintaining High RNA Quality in Surgical Lung Specimens
Thorac Cancer
January 23, 2025
Takashi Teishikata, Manabu Itoh, Yusuke Okamoto, Naofumi Miyahara, Chiho Nakashima, Koichiro Takahashi, Masafumi Hiratsuka, Keita Kai, Keiji Kamohara
RSC (Promega, Mannheim, Germany) platform and the commercially available
Maxwell RSC RNA FFPE kit
(
AS1440
,
Promega
). RNA was isolated according to the manufacturer's instructions. 2.5 Determination of RNA
(16)
Machine Learning Enabled Prediction of Biologically Relevant Gene Expression Using CT‐Based Radiomic Features in Non‐Small Cell Lung Cancer
Cancer Medicine
December 24, 2024
Shrey S. Sukhadia, Christopher Sadee, Olivier Gevaert, Shivashankar H. Nagaraj
Ribonucleic acid (RNA) was isolated from FFPE tissues using
Promega
Maxwell RSC RNA FFPE Kit
(cat#
AS1440
). The quality of the total RNA was evaluated by generating the DV200 score using Tapestation 4200 (Agilent
(17)
Proof of Concept Study: Comparison of Semi-Automated RNA Isolation Methods from Archived Formalin-Fixed, Paraffin-Embedded Tissues with Clinical Routine RNA Isolation Methods
Methods and Protocols
December 19, 2024
Patrick Hannibal Dalsbo Petersen, Jaslin Pallikkunnath James, Lene Buhl Riis, Claus Kim Høgdall, Estrid Vilma Høgdall
submerged in 300 µL of mineral oil using the
Maxwell® RSC RNA FFPE Kit
(
Promega
, Madison, WI, USA, Cat:
AS1440
) on a
Maxwell® RSC Instrument
(
Promega
) according to the manufacturer’s protocol [16]. The RNA was eluted
(18)
Cellular liquid biopsy provides unique chances for disease monitoring, preclinical model generation and therapy adjustment in rare salivary gland cancer patients
Molecular Oncology
October 5, 2024
Nataša Stojanović Gužvić, Florian Lüke, Steffi Treitschke, Andrea Coluccio, Martin Hoffmann, Giancarlo Feliciello, Adithi Ravikumar Varadarajan, Xin Lu, Kathrin Weidele, Catherine Botteron,
et al.
bp) and used for library preparation. RNA samples were extracted using the
MaxWell RSC RNA FFPE Kit
(
AS1440
;
Promega
) according to the manufacturer's protocols. Fifty ng of both DNA and RNA were then used for
(19)
Comprehensive transcriptomic analysis of prostate cancer lung metastases
PLOS ONE
August 15, 2024
Alireza Saraji, Katharina Wulf, Janine Stegmann-Frehse, Duan Kang, Anne Offermann, Danny Jonigk, Mark Philipp Kuehnel, Jutta Kirfel, Sven Perner, Verena Wilbeth Sailer
RNAase-free tube. RNA was isolated using the automatic bead-based
Maxwell RSC RNA FFPE Kit
(Cat. No.:
AS1440
,
Promega
) according to the manufacturer’s instructions. The RNA was eluted in water and then measured
(20)
Neoadjuvant nivolumab with or without relatlimab in resectable non-small-cell lung cancer: a randomized phase 2 trial
Nature Medicine
June 1, 2024
Martin Schuler, Kristof Cuppens, Till Plönes, Marcel Wiesweg, Bert Du Pont, Balazs Hegedus, Johannes Köster, Fabian Mairinger, Kaid Darwiche, Annette Paschen,
et al.
out semiautomatically on the Maxwell
purification
system (
Maxwell RSC RNA FFPE Kit
;
Promega
, cat. no.
AS1440
). All steps were performed following the respective protocol provided by the manufacturer. Total RNA
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Maxwell RSC RNA FFPE Kit from Promega
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