GoTaq Probe 1-Step RT-qPCR System A6120 from Promega

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ItemGoTaq Probe 1-Step RT-qPCR System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA6120
Quantity2ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Synergistic Virus Neutralizing Activities of European Black Elderberry Fruit Extract and Iota-Carrageenan Against SARS-CoV-2, Influenza A Virus and Respiratory Syncytial VirusNutrientsApril 10, 2026Christian Setz, Melanie Setz, Pia Rauch, Oskar Schleicher, Stephan Plattner, Andreas Grassauer, Ulrich Schubertvirus-containing samples was quantified by real-time PCR GoTaq® Probe qPCR one-step Kit from Promega (Cat: A6120, Madison, WI, USA). This kit allows reverse transcription, cDNA synthesis, and PCR amplification in

(2) Prenatal exposure to Zika virus shapes offspring neutrophil function in a sex-specific mannerNat CommunOctober 3, 2025Jiahui Ding, Anna Hu, Annie Thy Nguyen, Grace M. Swanson, Aditi Singh, Nicholas Adzibolosu, Diana Manchorova, Elizabeth Findeis, Anthony Maxwell, Yuan He, et al.was analyzed on a CFX96 C1000 qPCR system (Bio-Rad) using a one-step PCR mix (Promega, catalog no. A6120). Zika virus was detected using the following primer pair: forward 5′-CCGCTGCCCAACACAAG-3′ and reverse

(3) KRAS mutation promotes immune escape of lung adenocarcinoma via ZNF24/SLC7A5/PD-L1 axisBMC CancerSeptember 2, 2025Leilei Li, Qiang Feng, Ya Jiang, Lilin Yang, Hong Fang, Wenmang Xu, Yuanyuan Wang, Xinyan Pan, Julun Yangusing a NanoDrop 2000 spectrophotometer. Next, cDNA was synthesized using a fixed one-step RT-PCR kit (A6120, Promega, Shanghai, China) according to the manufacturer’s instructions. RT‐qPCR experiments were completed

(4) Engineered RNA-based activation system for coronavirus sensing in live cellsBioDesign ResearchSeptember 1, 2025Leiping Zeng, Christian Otero, Lei S. Qi(Zymo Research Cat# R1035). RT-qPCR was performed using the GoTaq Probe 1-Step RT-qPCR System (Promega Cat# A6120). 2.3 In vitro transcription (IVT) The IVT DNA templates were generated by PCR from the VERAS expression

(5) External quality assessment for molecular detection of sand fly-borne phleboviruses circulating in the Mediterranean BasinParasit VectorsMay 12, 2025Nazli Ayhan, Cecile Baronti, Laurence Thrion, Gioia Bongiorno, Carla Maia, Remi N. Charrel, CLIMOS WP2 Phlebovirus Working GroupMaxwell® 16 Viral Total Nucleic Acid Purification Kit (AS1150) (automated),Promega: GoTaq® Probe 1-Step RT-qPCR System (A6120),Promega: Access RT-PCR System (A1260) 3,200,60,5,Thermo Fisher Scientific: MagMAX™ Viral/Pathogen

(6) Synergistic Antiviral Activity of European Black Elderberry Fruit Extract and Quinine Against SARS-CoV-2 and Influenza A VirusaNutrientsMarch 29, 2025Christian Setz, Pia Rauch, Melanie Setz, Stephan Breitenberger, Stephan Plattner, Ulrich Schubertvirus-containing samples was quantified using the real-time PCR GoTaq® Probe qPCR one step Kit from Promega (Cat: A6120, Madison, WI, USA). This kit allows the reverse transcription, cDNA synthesis, and PCR amplification

(7) Functional Verification of Differentially Expressed Genes Following DENV2 Infection in Aedes aegyptiVirusesJanuary 6, 2025Xiaoli Chen, Xinyu Zhou, Xiaoxue Xie, Bo Li, Teng Zhao, Haotian Yu, Dan Xing, Jiahong Wu, Chunxiao Lidetected following mosquito RNA extraction using the GoTaq® Probe 1-Step System Assay Kit (Promega, A6120). The reaction format was as follows: 10 μL of 2× GoTaq® qPCR Master Mix, 0.4 μL of Go Script™ RT Mix

(8) Comparative Bioinformatic Analysis Reveals Conserved Regions in SARS-CoV-2 Genome for RAPID Pandemic ResponseInternational Journal of Molecular SciencesMay 25, 2024Marcela Viviana Karpuj, D. R. Shaytov, Yonat Shemer-Avni, Michael Gideon, Zakharia M. Frenkel, Sarit Sivanusing a synthetic RNA fragment employing the GoTaq® Probe 1-Step RT-qPCR System from Promega (Catalog # A6120 and A6121). It is important to note that our in-silico analysis (illustrated in Figure 1, steps 1–3)

(9) High-throughput surface epitope immunoaffinity isolation of extracellular vesicles and downstream analysisBiology Methods and ProtocolsMay 17, 2024Ramin Khanabdali, Michelle Mandrekar, Rick Grygiel, Phuoc-An Vo, Carlos Palma, Sara Nikseresht, Siena Barton, Mozhgan Shojaee, Sadman Bhuiyan, Kartini Asari, et al.transcription. Quantitation of mRNA was performed using GoTaq® Probe 1-Step RT-qPCR (Promega; Cat #A6120), following the manufacturer’s instructions, with the 7500 Fast Real-Time Instrument or QuantStudio™

(10) Establishing the role of BRCA1 in the diagnosis, prognosis and immune infiltrates of breast invasive cancer by bioinformatics analysis and experimental validationAging (Albany NY)January 31, 2024Leilei Li, Shuangyan Li, Xuyang Zhang, Liying Mei, Xueqin Fu, Min Dai, Na Weiinstructions of the RNA extraction kit (LS1040, Promega, Shanghai, China). Next, a fixed one-step RT-PCR kit (A6120, Promega, Shanghai, China) was utilized to synthesize cDNA. RT-qPCR assay was employed utilizing the

(11) Pulmonary SARS-CoV-2 infection leads to para-infectious immune activation in the brainFrontiers in immunologyJanuary 1, 2024Dunai C, Hetherington C, Boardman SA, Clark JJ, Sharma P, Subramaniam K, Tharmaratnam K, Needham EJ, Williams R, Huang Y, et al.(Invitrogen cat# 15596018, manufacturer’s protocol) using Promega’s GoTaq Probe 1-Step RT-qPCR system (cat#A6120, manufacturer’s protocol) on an Agilent AriaMx. Primers and FAM probes for SARS-CoV-2, cytokines, and

(12) Health disparities in COVID-19: immune and vascular changes are linked to disease severity and persist in a high-risk population in Riverside County, CaliforniaBMC public healthAugust 19, 2023Bergersen KV, Pham K, Li J, Ulrich MT, Merrill P, He Y, Alaama S, Qiu X, Harahap-Carrillo IS, Ichii K, et al.Catalog # 10,006,713). SARS-CoV-2 RNA was quantified using GoTaq® Probe 1-Step RT-qPCR System (Promega, A6120) according to the manufacturer’s protocol. 1000 copies/µL of 2019-nCoV plasmid (IDT, Catalog # 10,006,625)

(13) The Coronavirus Calendar (CoronaCal): a simplified SARS-CoV-2 test system for sampling and retrospective analysisFrontiers in EpidemiologyMay 23, 2023Manija A. Kazmi, David S. Thaler, Karina C. Åberg, Jordan M. Mattheisen, Thomas Huber, Thomas P. SakmarVacConnectors (Qiagen #19407). RT-qPCR was carried out using the GoTaq Probe 1 Step qPCR system (Promega #A6120) on an Agilent AriaMx qPCR instrument. All primers, probes and control plasmids were from IDT Integrated

(14) Diagnostic accuracy of the Panbio COVID-19 antigen rapid test device for SARS-CoV-2 detection in Kenya, 2021: A field evaluationPLOS ONEJanuary 25, 2023Jack Karuga Irungu, Peninah Munyua, Caroline Ochieng, Bonventure Juma, Patrick Amoth, Francis Kuria, John Kiiru, Lyndah Makayotto, Ahmed Abade, Marc Bulterys, et al.rRT-PCR master mix (Thermo Fisher Scientific) or using the GoTaq® Probe 1-Step RT-qPCR System (Promega A6120). Both systems are compatible with the rRT-PCR CDC IDT kit. Each of the 15μL reaction volumes contained

(15) Effects of constant temperature and daily fluctuating temperature on the transovarial transmission and life cycle of Aedes albopictus infected with Zika virusFrontiers in MicrobiologyJanuary 4, 2023Xian-yi Jian, Yu-ting Jiang, Miao Wang, Nan Jia, Tong Cai, Dan Xing, Chun-xiao Li, Tong-yan Zhao, Xiao-xia Guo, Jia-hong Wudetection The virus was quantified by qRT-PCR using the GoTaq® Probe 1-Step RT-qPCR System (Promega A6120). The PCR primers included a pair of universal primers and probe (Pyke et al., 2014), F: 5'-AAGTTTGCATGCTCCAAGAAAAT-3',

(16) Broad-spectrum CRISPR-mediated inhibition of SARS-CoV-2 variants and endemic coronaviruses in vitroNature CommunicationsMay 19, 2022Leiping Zeng, Yanxia Liu, Xammy Huu Nguyenla, Timothy R. Abbott, Mengting Han, Yanyu Zhu, Augustine Chemparathy, Xueqiu Lin, Xinyi Chen, Haifeng Wang, et al.(Zymo Research Cat# R1035). RT-qPCR was performed using the GoTaq Probe 1-Step RT-qPCR System (Promega Cat# A6120). RT-qPCR primers and probes are listed in Supplementary Data 2. To quantify viral RNA abundance in

(17) Development and Testing of a Low-Cost Inactivation Buffer That Allows for Direct SARS-CoV-2 Detection in SalivaVaccines (Basel)May 6, 2022Brandon Bustos-Garcia, Sylvia Garza-Manero, Nallely Cano-Dominguez, Dulce Maria Lopez-Sanchez, Gonzalo Salgado-Montes de Oca, Alfonso Salgado-Aguayo, Felix Recillas-Targa, Santiago Avila-Rios, Victor Julian Valdes5 µL was used as template in a 20 µL reaction using the GoTaq Probe 1-Step RT-qPCR System Cat. No. A6120 (Promega, Madison, WI, USA). The N1-FAM, N2-FAM, and hRP-FAM oligo/probe assays were from the 2019-nCoV

(18) Crimean-Congo hemorrhagic fever virus in ticks collected from imported camels in EgyptSaudi Journal of Biological SciencesApril 1, 2022Hager A. Bendary, Fatma Rasslan, Milton Wainwright, Saleh Alfarraj, Ali M. Zaki, Abeer K. Abdulallperformed in wells triplicate of 48-well plates using the GoTaq Probe 1-Step RT-qPCR System (Cat No. A6120, Promega, Germany). A 5 μL of sample and 25-μL reactions run were used on the Applied Biosystems StepOne™ Real-Time PCR System

(19) Surveillance of SARS-CoV-2 variants of concern by identification of single nucleotide polymorphisms in the spike protein by a multiplex real-time PCRJournal of Virological MethodsFebruary 1, 2022Laksiri Gomes et al.(2021)). The multiplex real-time PCR was performed using GoTaq® Probe 1-Step RT-qPCR System (Promega, Cat: A6120) in CFX96 Touch Real-Time PCR Detection System (BIORAD, USA). The reactions consisted of 25 μl volumes

(20) SARS-CoV-2 RNA Detected in Abdominal Insufflation Samples During Laparoscopic SurgeryEuropean UrologyJanuary 1, 2022Ketan K. Badani et al.#2019-nCoVEUA-01) and Mastermix from a Promega GoTaq Probe 1-Step RT-qPCR kit (Promega, Madison, WI, USA; catalog #A6120) for detection of SARS-CoV-2. RNA extraction and qPCR were also concomitantly conducted in the same

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GoTaq Probe 1-Step RT-qPCR System

GoTaq Probe 1-Step RT-qPCR System from Promega

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GoTaq Probe 1-Step RT-qPCR System from Promega

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