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CellTiter-Glo 2.0 Assay
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CellTiter-Glo 2.0 Assay
CellTiter-Glo 2.0 Assay
CellTiter-Glo 2.0 Assay from Promega
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Citations:
(>1k)
Figures:
(4)
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CellTiter-Glo 2.0 Assay
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Description
The CellTiter-Glo 2.0 Assay provides a homogeneous method to determine the number of viable cells in culture by quantifying ATP, which indicates the presence of metabolically active cells
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
CellTiter-Glo 2.0 Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G9241
Quantity
10ml
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (1152)
(1)
Tramadol induced hypoxia signaling and paraptosis-like cell death in breast cancer cells via HIF-1α and ATF4 dependent pathways
Redox Report
December 31, 2026
Zih-Syuan Wu, Shih-Ming Huang, Yi-Hsuan Huang
Madison, WI, USA), whereas cell viability was measured using the
CellTiter-Glo® 2.0 Assay
(catalog no.:
G9241
;
Promega
).
Fluorescence
or
luminescence
signals were detected using a Varioskan LUX plate reader (
Thermo Fisher Scientific
(2)
3D matrix stiffness drives energy metabolism to orchestrate stem cell osteogenesis via microtubule acetylation and mitochondrial dynamics
Bioactive Materials
November 1, 2026
Zhijie Yang, Jing Na, Yu Liu, Gan Yue, Fei Song, Chiyu Li, Qiusheng Shi, Yubo Fan, Lisha Zheng
cells per 100 μl. 100 μl cell suspension and 100 μl CellTiter-Glo® 2.0
Cell Viability Assay
reagent (
G9241
,
Promega
, WI, USA) were mixed rapidly in opaque 96-plates and the luminescent output was detected by
(3)
A critical role for mitochondrial dynamics in cigarette smoke condensate-induced RPE senescence
Free Radical Biology and Medicine
October 1, 2026
Qi Zhou, Zunyi Zhang, Yinga Wu, Chunmin Dong, Chen-Chuan Wu, James T. Handa, Jing Ma, Shusheng Wang
MitoSOX loading. Cellular ATP level was measured as described in the
CellTiter-Glo 2.0 Assay
kit (
Promega
G9242
). Briefly, ARPE-19 cells were seeded using opaque-walled 96-well plates with 100 μl of culture medium,
(4)
The long non-coding RNA, CyKILRb, acts as a competing endogenous RNA for tumor suppressive microRNAs to augment oncogenic phenotypes via induction of the PIK3R2/PI3K/AKT axis
Molecular Therapy Nucleic Acids
September 8, 2026
Xiujie Xie, H. Patrick Macknight, Barbara Dziegielewska, Amy L. Lu, Ngoc Thanh Vu, Charles E. Chalfant
Cell Titer-Glo 2.0
Assay
Kit and
Dual-Luciferase Reporter Assay System
were purchased from
Promega
(
G9242
and
E1910
). Biotin labled miR-control and miR-3151-5p were synthesized from Horizon. The
mycoplasma
(5)
Mitochondrial profiling across macrophage states reveals inhibition of IL-4/IL-13 reprogramming by the integrated stress response
Sci Adv
August 21, 2026
Joan Blanco-Fernandez, Miriam Lisci, Mads M. Foged, Chloé Chapuis, Tim Pflästerer, Tatjana Kleele, Alexis A. Jourdain
stimulation, ATP was quantified using the
CellTiter-Glo luminescent cell viability assay
kit (
Promega
,
G9242
) according to the manufacturer’s instructions. Briefly, 100 μl of reconstituted CellTiter-Glo Reagent
(6)
TP53 mutation drives unique transcriptional and functional vulnerabilities independent of del(17p) in multiple myeloma
iScience
August 21, 2026
Dimitrios Tsallos, Nemo Ikonen, Juho J. Miettinen, Muntasir Mamun Majumder, Samuli Eldfors, Imre Västrik, Alun Parsons, Minna Suvela, Katie Dunphy, Paul Dowling,
et al.
Media,
Cytiva
,Cat# 11778538 Conditioned medium,This paper,– CellTiter-Glo® 2.0 reagent,
Promega Corporation
,Cat#
G9243
Compounds used in this paper are available on Table S7,–,– ,, Critical commercial
assays
,Critical
(7)
The requirement of MALT1 activity for the growth of adult T cell leukemia/lymphoma
iScience
August 21, 2026
Ayako Kamiunten, Daisuke Morishita, Takuro Kameda, Midori Sugiyama, Shunsuke Ebara, Yasunori Kogure, Akio Mizutani, Takaharu Hirayama, Tomohiro Kawamoto, Yotaro Ochi,
et al.
commercial
assays
,Critical commercial
assays
,, CellTiter-Glo® 2.0
Cell Viability Assay
,
Promega
,#
G9242
SureSelect
Custom Kit,
Agilent Technologies
,N/A QuantiChrom™ Lactate Dehydrogenase Kit,BioAssay Systems,#D2DH-100
Human
(8)
A host-centric morphological profiling approach to identify repurposed antiviral drugs
iScience
August 21, 2026
Elin Asp, Jonne Rietdijk, Marianna Tampere, Hanna Axelsson, Duncan Njenda, Swapnil Potdar, Adelinn Kalman, Polina Georgieva, Maris Lapins, Flavio Ballante,
et al.
proteins" ,, SPECs
drug repurposing
library,https://www.specs.net/,N/A CellTiter-Glo,
Promega
,Ref.
G9243
Paraformaldehyde,
Thermo Scientific
,Ref.
28908
BSA,Sigma,Ref. A8022 Hoechst 33342,
Invitrogen
,Ref.
(9)
Early CCR2-dependent monocyte recruitment defines a critical neuroinflammatory window after subarachnoid hemorrhage
Front Immunol
August 14, 2026
Dong Su Kang, Seung Hyeok Seok, Seung-Hoon Lee, Yi Rang Na
conditions. Cell viability was assessed using the CellTiter-Glo® 2.0
Cell Viability Assay
(
Promega
,
G9241
) according to the manufacturer’s instructions.
Luminescence
was measured using a
BioTek
Synergy™ Mx
(10)
Synergistic lethality of combination treatment with Trop2-directed antibody-drug conjugate sacituzumab govitecan and TRAIL agonists in triple negative breast cancer
Breast Cancer Res
August 14, 2026
Yonit A. Addissie, Yoshimi E. Greer, Jayakumar Nair, Stanley Lipkowitz
cells/total brightfield
cell number
)*100). Cell viability was measured using CellTiter-Glo 2.0 (cat#
G9242
) as directed by
Promega
. Briefly, 2,500 cells per well were plated in a 96-well plates and treated on
(11)
6-(2-Aminoethyl)-6H-indolo[2,3-b]quinoxalines as Promising Compounds Capable of Binding to FLT3 (D835V) Kinase
Biomolecules
August 12, 2026
Igor A. Schepetkin, Alexander V. Uvarov, Egor A. Evriinov, Andrei I. Khlebnikov
Assay Cytotoxicity was analyzed with a
CellTiter-Glo Luminescent Cell Viability Assay
Kit from
Promega
(cat. #
G9241
; Madison, WI, USA) according to the manufacturer’s protocol.
THP-1 cells
were treated with varying concentrations
(12)
Acquired resistance to the RAS(ON) multi-selective inhibitor daraxonrasib guides rational combination therapy strategies in pancreatic cancer
Nat Med
August 11, 2026
Ida Aronchik, Sumit Kar, Yongxian Zhuang, Ethan Ahler, Lick Pui Lai, Vidya Seshadri, Yu Chi Yang, Ashenafi Bulle, Marie Menard, Biswadeep Nayak,
et al.
after treatment
(T5) using the Envision Plate Reader (PerkinElmer). Then, 25 μl of CellTiter-Glo 2.0 reagent (
G9243
,
Promega
) was added to each well. Plates were shaken at 450 rpm for 2 min at room temperature and then
(13)
Umbilical cord-derived mitochondrial transplantation restores tendon structure and function in a preclinical model of achilles tendinopathy
J Transl Med
August 7, 2026
Da-Yoon Kim, Mi Jin Kim, Min-Jeong Park, Mina Lim, Eun-Seo Back, Chang-Koo Yun, Seong Hun Kim, Hyuk Nam Kwon, Chaeyoung Lee, Kyunghoon Min,
et al.
mixture was incubated for 45 min at 37°C. After
incubation
, 100 µL of CellTiter-Glo® 2.0 reagent (Cat#
G9243
,
Promega
, Madison, WI, USA) was added to each well, followed by shaking for 2 min.
Luminescence
was
(14)
LCK-targeting molecular glues overcome resistance to inhibitor-based therapy in T-cell acute lymphoblastic leukemia
Blood Journal
August 6, 2026
Satoshi Yoshimura, Satoshi Yoshimura, Justin T. Seffernick, Justin T Seffernick, Jamie A. Jarusiewicz, Jamie Jarusiewicz, Angelina Aggarwal, Angelina Li, DongGeun Lee, DongGeun Yang,
et al.
provided in the supplemental Methods. CTG
assay
for
T-ALL
cell lines CellTiter-Glo (CTG; catalog no.
G9243
;
Promega
;) was used as a proxy
measure
of
cell number
based on ATP content.
T-ALL
cell lines were plated
(15)
A dual pronged approach to cancer immune exclusion: tumor-derived LAIR-1 simultaneously drives fibrosis and blocks immune recruitment in glioma
ResearchSquare
August 5, 2026
Maria Luisa Lowenstein, Maria Luisa Varela, Henry Kast, Marisa West, Daiana Perez Visñuk, Daiana Perez Springer, Sadhakshi Springer, Sadhakshi Raghuram, Yichen Wang, Brandon McClellan,
et al.
Proliferation Assay Cell proliferation was measured using CellTiter-Glo(R) 2.0 Viability
Assay
(
Promega
,
G9242
) following the manufacturer’s instructions RNA Scope
RNAscope in-situ hybridization
was performed
(16)
Terminal sterilization of food-grade coatings for cellular agriculture: robustness against water quality and regulatory feasibility
Biotechnol Rep (Amst)
August 4, 2026
Hiroaki Hatano, Misaki Sawada, Naomi Nakamura, Misaki Kitsukawa, Azusa Hayakawa, Hiroaki Kondo, Ikko Kawashima
was quantified by measuring intracellular ATP levels using CellTiter-Glo® 2.0
Cell Viability Assay
(
G9241
,
Promega
, WI, USA).
Luminescence
was measured using a Nivo multimode plate reader (PerkinElmer, MA,
(17)
Combination AURKA and WEE1 Inhibition Exhibits Efficacy in EGFR or Pan-ERBB Inhibitor–Resistant Head and Neck Squamous Cell Carcinoma
Cancer Res Commun
August 1, 2026
Flaviane N. Silva, Jong Woo Lee, Theodore T. Nguyen, Fiona M. Goeckel, Tetyana Bagnyukova, Hossein Borghaei, Erica A. Golemis, Barbara A. Burtness
incubation
, cell viability was assessed using a CellTiter-Glo 2.0
Cell Viability Assay
(
Promega
, item
G9241
). Plates were equilibrated at room temperature for 20 minutes. Sixty microliters of CellTiter-Glo per
(18)
Doxorubicin‐Induced Cytotoxicity in Adipose‐Derived Stem Cells Is Associated With Altered SAPK/JNK Signaling
FASEB J
July 30, 2026
Aleksandra Skubis‐Sikora, Kinga Pogoda‐Mieszczak, Bartosz Sikora, Edyta Bogunia, Aleksandra Bryzek, Piotr Czekaj
well in 96‐well black plates. The effect of Dox was analyzed using the
CellTiter‐Glo 2.0 assay
kit (
G9241
;
Promega
, Madison, WI, USA) according to the manufacturer's protocol. The bioluminescent
CellTiter‐Glo 2.0 assay
(19)
Distinct metabolic dependency on BCAA defines cancer stemness and malignancy in human triple-negative breast cancer
Cell Reports
July 28, 2026
Kenkyo Matsuura, Ririko Shinonaga, Mizuki Yamamoto, Yoshiki Yamamoto, Hsin Chen, Ayaka Maeno, Kayo Okuda, Haruki Inoue, Sota Imotani, John Glushka,
et al.
collected and an equal volume of CellTiter-Glo 2.0 cell proliferation
assay
reagent (
Promega
, cat#
G9243
) was added to the
cell culture
. Samples were transferred to a 96 well white plate, and the luminescent
(20)
RelB drives integrin-mediated stress tolerance and relapse in high-grade serous ovarian cancer
Cell Reports
July 28, 2026
Omar Lujano-Olazaba, Mikella Robinson, Gregory J. Jordan, Sofia Howe, Anna Platen, Cassidy Lucht, Dishant Vandra, Cecilia Gallo, Mena Shammas, Katelyn Shelby,
et al.
detachment",Gibco,Cat#25-200-114 Critical commercial
assays
,, CellTiter-Glo 2.0 Viability
assay
,
Promega
,Cat#
G9242
DuoSet ELISA Kit (
TWEAK),R
&D Systems,Cat#DY1090
NucleoSpin RNA Plus
Kit,
Macherey-Nagel
,Cat#740984.50 High-Capacity
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CellTiter-Glo 2.0 Assay from Promega
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