ROS-Glo H2O2 Assay from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG8821
Quantity50ml
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Ascorbate mitigates oxidative stress and hemin cytotoxicity in heme oxygenase-1 deficiencyJournal of Biological ChemistryNovember 1, 2025Lea-Sophie Berendes, Petra Schulze Schulze Westhoff, Ann-Marie Tobinski, Jorge A. Narvaez Pardo, Victoria Wingert, Manfred Fobker, Saskia Biskup, Anja Seelhöfer, Veerle Van Marck, Barbara Heitplatz, et al.levels, a bioluminescent, boronate-probe-based assay was used according to the manufacturer’s protocol (#G8821, Promega, Madison, WI, USA). In brief, 20,000 (LCL) or 15,000 (HEK293T) cells per genotype we seeded

(2) Sigma-1 receptor counteracts non-cell-autonomous poly-PR-induced astrocytic oxidative stress in C9orf72 ALSRedox biologyNovember 1, 2025Wu HC, Huang TW, Weng EF, Lin CY, Su TP, Wu HE, Wang SM.were subjected to Western blot analysis. 2.7 H2O2 assay Following the manufacturer's instructions (G8821, Promega, WI, USA), cells and conditioned media were incubated with the H2O2 Substrate Solution for

(3) Integration of co-culture conditions and 3D gelatin methacryloyl hydrogels to improve human-induced pluripotent stem cells-derived cardiomyocytes maturationFront Bioeng BiotechnolJuly 14, 2025Ilaria Gisone, Monica Boffito, Elisa Persiani, Roberta Pappalardo, Elisa Ceccherini, Andrea Alliaud, Manuela Cabiati, Rossella Laurano, Letizia Guiducci, Chiara Caselli, et al.the absorbance of the samples at 490 or 492 nm). 2.7.3 ROS assay The ROS-Glo™ H2O2 assay (Promega, G8821) is a luminescent test that measures the Reactive Oxygen Species (ROS) produced and released by cells

(4) NRF2 supports non-small cell lung cancer growth independently of CBP/p300-enhanced glutathione synthesisEMBO ReportsMay 14, 2025Ryan J Conrad, James A Mondo, Mike Lingjue Wang, Peter S Liu, Zijuan Lai, Feroza K Choudhury, Qingling Li, Weng Ruh Wong, James Lee, Frances Shanahan, et al.BCA Protein Assay Kit,Pierce,A65453 CellTiter-Glo Luminescent Cell Viability Assay,Promega,G7571 ROS-Glo H2O2 Assay,Promega,G8821 RNeasy Plus Mini Kit,Qiagen,74136 TaqMan™ Gene Expression Master Mix,Thermo,4369016 Qubit RNA HS Assay Kit

(5) Myoglobin-derived iron causes wound enlargement and impaired regeneration in pressure injuries of muscleeLifeJune 2, 2023Nasir NJM, Heemskerk H, Jenkins J, Hamadee NH, Bunte R, Tucker-Kellogg L.,Mouse Luminex Discovery Assay,R&D Systems,LXSAMSM-21, Commercial assay or kit,ROS-Glo H2O2 Assay,Promega,G8821, Commercial assay or kit,Efferocytosis Assay Kit,Cayman Chemical,601770, Commercial assay or kit,MycoAlert PLUS mycoplasma detection kit

(6) PARP inhibition and pharmacological ascorbate demonstrate synergy in castration-resistant prostate cancerbioRxivMarch 26, 2023Nicolas Gordon, Peter T. Gallagher, Neermala Poudel Neupane, Amy C. Mandigo, Jennifer K. McCann, Emanuela Dylgjeri, Irina Vasilevskaya, Christopher McNair, Channing J. Paller, Wm. Kevin Kelly, et al.previously described. After treatment, ROS levels were assessed using the ROS-Glo H2O2 Assay (Promega G8821) per manufacturer’s instructions. Briefly, cells were pre-incubated with the ROS substrate for 2 hours

(7) Enhancement of reactive oxygen species production in triple negative breast cancer cells treated with electric pulses and resveratrolExploration of Targeted Anti-tumor TherapyFebruary 28, 2023Pragatheiswar Giri, Ignacio G. Camarillo, Raji SundararajanAfter 18 h incubation, 20 μL of hydrogen peroxide (H2O2; G882B, Promega, USA) substrate from ROS-GloTM (G8821, Promega, USA) was added and further incubated for 6 h. The luciferin precursor was formed by the direct

(8) RB/E2F1 as a Master Regulator of Cancer Cell Metabolism in Advanced DiseaseCancer DiscoverySeptember 1, 2021Amy C. Mandigo et al.positive control. Relative ROS levels were measured by fluorescence using the ROS-Glo Assay (Promega, G8821). Fluorescence was measure on a BioTek Synergy HT microplate reader and analysis using BioTek Gen5 2.09

(9) Tuning the Immunostimulation Properties of Cationic Lipid Nanocarriers for Nucleic Acid DeliveryFrontiers in ImmunologyAugust 23, 2021Arindam K. Dey, Adrien Nougarède, Flora Clément, Carole Fournier, Evelyne Jouvin-Marche, Marie Escudé, Dorothée Jary, Fabrice P. Navarro, Patrice N. Marchestandard curve. ROS production by BMDMs and BMDCs was determined by ROS-Glo™ H2O2 assay kit (Promega, G8821). The cells were cultured at 5 x 104 cell/mL concentration in a 96-well plate, exposed to nanocarriers

(10) Selective activation of PFKL suppresses the phagocytic oxidative burstCellAugust 19, 2021Amara N, Cooper MP, Voronkova MA, Webb BA, Lynch EM, Kollman JM, Ma T, Yu K, Lai Z, Sangaraju D, et al.96-well multi-array Tissue Culture Kit",Meso Scale Discovery,K151AGB-2 ROS-Glo H2O2 assay,Promega,G8821 NAD/NADH-Glo assay,Promega,G9082 NADP/NADPH-Glo assay,Promega,G9072 EasySep human monocyte isolation kit

(11) Pathway-based classification of glioblastoma uncovers a mitochondrial subtype with therapeutic vulnerabilitiesNature cancerFebruary 1, 2021Garofano L, Migliozzi S, Oh YT, D'Angelo F, Najac RD, Ko A, Frangaj B, Caruso FP, Yu K, Yuan J, et al.experiments for glutamine consumption. Quantification of ROS was performed using ROS-Glo Assay (PROMEGA, no. G8821) according to the manufacturer’s instructions. Briefly, cells were plated at a density of 7,000 per

(12) Impact of Gold Nanoparticles on the Functions of Macrophages and Dendritic CellsCellsJanuary 7, 2021Dey AK, Gonon A, Pécheur EI, Pezet M, Villiers C, Marche PN.ROS production by BMDMs and BMDCs was determined by the ROS-Glo™ H2 O2 Assay kit (Promega, cat. no.: G8821). The cells were cultured at a 5 × 104 cells/mL concentration in 96-well plates, exposed to AuNPs 24

(13) 3D Culture Models with CRISPR Screens Reveal Hyperactive NRF2 as a Prerequisite for Spheroid Formation via Regulation of Proliferation and FerroptosisMolecular cellDecember 3, 2020Takahashi N, Cho P, Selfors LM, Kuiken HJ, Kaul R, Fujiwara T, Harris IS, Zhang T, Gygi SP, Brugge JS.E0554S QuikChange II XL Site-Directed Mutagenesis Kit,Agilent,Cat# 200522 ROS-Glo™ H2O2 Assay,Promega,Cat# G8821 OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit,Cell Biolabs,Cat# STA-344 GSH/GSSG-Glo™ Assay,Promega,Cat#

(14) AR ubiquitination induced by the curcumin analog suppresses growth of temozolomide-resistant glioblastoma through disrupting GPX4-Mediated redox homeostasisRedox BiologyDecember 26, 2019Tzu-Chi Chen, Jian-Ying Chuang, Chiung-Yuan Ko, Tzu-Jen Kao, Pei-Yu Yang, Chun-Hui Yu, Ming-Sheng Liu, Siou-Lian Hu, Yu-Ting Tsai, Hardy Chan, Wen-Chang Chang, Tsung-I. Hsuby the GuavaSoft software (MerckMillipore). 2.8 H2O2 and GSH/GSSG analysis The H2O2-Glo® (Catalog #G8821) and GSH/GSSG-Glo® (Catalog #V6612) assay kits were purchased from Promega Inc. and used according to

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