NanoBRET Nano-Glo Detection System N1661 from Promega

Product Specs
ItemNanoBRET Nano-Glo Detection System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1661
Quantity200 assays

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A degron-mimicking molecular glue drives CRBN homo-dimerization and degradationNature CommunicationsNovember 19, 2025Gerasimos Langousis, Pablo Gainza, Moritz Hunkeler, Despoina Kapsitidou, Etienne J. Donckele, Stefano Annunziato, Lars Wiedmer, Katherine F. M. Jones, Bradley DeMarco, Chao Quan, et al.added, and donor/acceptor signals were measured according to manufacturer instructions (Promega Cat#N1661) at the PHERAstar FSX plate reader (BMG LABTECH). The NanoBRET corrected ratio, including background

(2) Ebola virus VP35 NNLNS motif modulates viral RNA synthesis and MIB2-mediated signalingProceedings of the National Academy of SciencesSeptember 30, 2025Grace Uwase, Priya Luthra, Olivia A. Vogel, Jyoti Batra, Bruno A. La Rosa, Kathleen C. F. Sheehan, Oam Khatavkar, Jacqueline E. Payton, Robert A. Davey, Nevan J. Krogan, et al.included in the kit. Subsequent experiments were run using the NanoBRET Nano-Glo Detection System (Promega N1661). For ubiquitination experiments with transient expression, 1 × 106 293T cells were transfected in six-well

(3) A hexamerization-enhanced, Fc-silenced agonistic CD27 antibody amplifies T-cell effector functions as single agent and in combination with PD-1 blockadeSci RepJuly 24, 2025Işıl Altıntaş, Kristina B. Nürmberger, Andrea Imle, Anna-Lena Krause, Marike M. van Beuge, Aras Toker, Andreea Ioan-Facsinay, Jordan Blum, Alexander Muik, Frank J. Beurskens, et al.GraphPad Prism software. BRET assay BRET assays were performed using a NanoBRET System (Promega, N1661) and K562_hCD27 cells generated from K562 cells (ATCC-CCL-243;RRID:CVCL_0004). The assays were performed

(4) Nf2-FAK signaling axis is critical for cranial bone ossification and regenerationNat CommunMarch 12, 2025Junguang Liao, Yuping Huang, Fuju Sun, Chenggong Zheng, Yifeng Yao, Cui Zhang, Chenhe Zhou, Xingen Zhang, Mengrui Wu, Guiqian Chensignal was detected 48 h post-transfection immediately after adding 25 μl Nano-Glo® Live-cell Reagent (N1661, Promega) using Varioskan Flash Full Wavelength Scanning Multifunction Reader (Thermofisher) at ambient

(5) Application of bioluminescence resonance energy transfer assays in primary mouse neuronal culturesSTAR ProtocolsSeptember 20, 2024Hanh T.M. Hoang, Kiran George, Mohiuddin AhmadG9801 NanoBRET Nano-Glo substrate,Promega,Cat# N1571 NanoBRET Nano-Glo detection systems,Promega,Cat# N1661 QuickTiter Lentivirus Titer Kit,Cell Biolabs,Cat# VPK-107 ,, Experimental models: Cell lines,Experimental models

(6) Lysine deserts and cullin-RING ligase receptors: Navigating untrodden paths in proteostasisiScienceNovember 17, 2023Natalia A. Szulc, Małgorzata Piechota, Lilla Biriczová, Pankaj Thapa, Wojciech Pokrzywa(BRET) ubiquitination assay was performed using the NanoBRET Nano-Glo Detection Systems (Standard) (N1661, Promega) according to the manufacturer’s guidelines. Briefly, the HaloTag 618 ligand was added to the

(7) Protocol for measuring BRAF autoinhibition in live cells using a proximity-based NanoBRET assaySTAR ProtocAugust 16, 2023Russell Spencer-Smith, Deborah K. Morrisoncommercial assays,Critical commercial assays,Critical commercial assays ,, NanoBRET kit,Promega,cat# N1661 ,, Deposited Data,Deposited Data,Deposited Data ,, Raw data files,This paper,Mendelely Datahttps://doi.org/10.17632/p2bhw868dn.1 ,, Experimental models

(8) Pathway selectivity in Frizzleds is achieved by conserved micro-switches defining pathway-determining, active conformationsNature communicationsJuly 29, 2023Grätz L, Kowalski-Jahn M, Scharf MM, Kozielewicz P, Jahn M, Bous J, Lambert NA, Gloriam DE, Schulte G.microtiter plates (Thermo Fisher Scientific). After 24 h, 10 nM C59 and 50 nM HaloTag NanoBRET 618 ligand (#N1661, Promega) were added to the cells. Next day, cells were washed once with HBSS and incubated with 100

(9) Phosphomimetics at Ser199/Ser202/Thr205 in Tau Impairs Axonal Transport in Rat Hippocampal NeuronsMolecular NeurobiologyJune 1, 2023Kyle R. Christensen, Benjamin Combs, Collin Richards, Tessa Grabinski, Mohammed M. Alhadidy, Nicholas M. Kanaana control according to the manufacturer’s instructions (Promega NanoBRET Nano-Glo Detection System N1661) [37]. Replated cells were incubated overnight. The next day, the cell culture media was removed and

(10) RASopathy mutations provide functional insight into the BRAF cysteine-rich domain and reveal the importance of autoinhibition in BRAF regulationMolecular cellNovember 17, 2022Spencer-Smith R, Terrell EM, Insinna C, Agamasu C, Wagner ME, Ritt DA, Stauffer J, Stephen AG, Morrison DK.Assays ,, CellTiter-Glo 2.0,Promega,cat# G9241 QuickChange II Kit Agilent,Agilent,cat# 200523 NanoBRET Kit,Promega,cat# N1661 ,, Deposited Data,Deposited Data,Deposited Data ,, Raw data files,This paper,Mendeley Data: https://doi.org/10.17632/d89bbp4k2k.1 ,, Experimental Models

(11) Galectin-9 restricts hepatitis B virus replication via p62/SQSTM1-mediated selective autophagy of viral core proteinsNature communicationsJanuary 27, 2022Miyakawa K, Nishi M, Ogawa M, Matsunaga S, Sugiyama M, Nishitsuji H, Kimura H, Ohnishi M, Watashi K, Shimotohno K, et al.respectively. NanoBRET activity was measured using the NanoBRET Nano-Glo Detection System (Promega, #N1661) on a GloMax Discovery system (Promega, software version 3.2.3). Transfection-based HBV production

(12) An ionic lock and a hydrophobic zipper mediate the coupling between an insect pheromone receptor BmOR3 and downstream effectorsThe Journal of biological chemistryOctober 1, 2021Lin JY, Yang Z, Yang C, Du JX, Yang F, Cheng J, Pan W, Zhang SJ, Yan X, Wang J, et al.cAMP assay reagent (E1290), Rluc substrate Coelenterazine h (S2011), and Nluc substrate furimazine (N1661) were purchased from Promega. TC-FlAsH II In-Cell Tetracysteine Tag Detection Kit (T34561) was purchased

(13) A BRET-based assay reveals collagen–Hsp47 interaction dynamics in the endoplasmic reticulum and small-molecule inhibition of this interactionJournal of Biological ChemistryNovember 1, 2019Shinya Ito, Masazumi Saito, Masahito Yoshida, Koh Takeuchi, Takayuki Doi, Kazuhiro NagataSigma–Aldrich). BRET assay The BRET assay was performed using reagents from Promega (catalog no. N1661) as described previously (10). In brief, HEK293 or HeLa cells were cultured in Dulbecco's modified Eagle's

(14) Discovery of Pyrrolo[3,2-d]pyrimidin-4-one Derivatives as a New Class of Potent and Cell-Active Inhibitors of P300/CBP-Associated Factor BromodomainJournal of Medicinal ChemistryMay 9, 2019Luyi Huang et al.#3917) in the absence (blank control) or the presence of 100 nM NanoBRET 618 fluorescent ligand (Promega N1661). Compounds or DMSO (vehicle control) were diluted using media and then added into a 96-well plate at

(15) Developing a Biased Unmatched Bivalent Ligand (BUmBL) Design Strategy to Target the GPCR Homodimer Allosteric Signaling (cAMP over β-Arrestin 2 Recruitment) Within the Melanocortin ReceptorsJournal of Medicinal ChemistryJanuary 10, 2019Cody J. Lensing, Katie T. Freeman, Sathya M. Schnell, Robert C. Speth, Adam T. Zarth, Carrie Haskell-Luevanothe assay buffer and cell medium was removed by aspiration. Then 20 µL of Bright-Glo (Promega, Cat # N1661) diluted 20-fold in assay buffer was added to each well and incubated to 15–20 minutes. After incubation, luminescence

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NanoBRET Nano-Glo Detection System

NanoBRET Nano-Glo Detection System from Promega

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NanoBRET Nano-Glo Detection System from Promega

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