Maxwell RSC simplyRNA Cells Kit from Promega

Product Specs
ItemMaxwell RSC simplyRNA Cells Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1390
Quantity48 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA
Sample TypeFresh or frozen cells

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Intragenic Transcription from Defective HIV Proviruses Triggers Interferon Responses in Myeloid CellsbioRxivApril 7, 2026Jonathan Kilroy, Aparna Deokar, Samantha Patalano, Juan Fuxman Bass, Manish Sagar, Suryaram Gummuluru, Andrew J. Hendersonsome experiments, RNA was isolated using the Maxwell RSC simplyRNA Cells kit (Promega, catalog number: AS1390) and DNA was isolated using the Maxwell RSC Genomic DNA kit (Promega, catalog number: AS1880) according

(2) Posttranscriptional reprogramming controls MASLD progression through chronic ER stress adaptationSci AdvApril 3, 2026Eulalia Belloc, Vittorio Calderone, Salvador Naranjo-Suarez, Lidia Mateo, Judit Martin, Florencia Malizia, Annarita Sibilio, Veronica Chanes, Marta Ramirez-Pedraza, M. Eugenia Delgado, et al.following the manufacturer’s instructions. RNA was extracted with Maxwell RSC simply RNA cells kit (AS1390, Promega). Tumor xenografts and allografts Tumor cells were injected subcutaneously (right and left

(3) Alternative splicing in pediatric central nervous system tumors highlights oncofetal candidate CLK1 exon 4Neuro-Oncology PediatricsMarch 30, 2026Ammar S Naqvi, Patricia J Sullivan, Ryan J Corbett, Priyanka Sehgal, Karina L Conkrite, Komal S Rathi, Brian M Ennis, Katharina E Hayer, Bo Zhang, Miguel A Brown, et al.(qRT-PCR) Total RNA was isolated and treated with DNAse using the Maxwell RSC simplyRNA Cells kit (Promega, AS1390) with the Maxwell RSC48 Instrument (Promega) per the manufacturer’s instructions. Next, 2 μg of RNA

(4) Lipidomics of allantoic fluid collected by allantocentesis indicates a possible mechanism underlying partial litter loss in multifetal pregnancies of ewesScientific ReportsMarch 18, 2026Tamir Alon, Maya Ross, Jen Spirer, Priscila Dos Santos Silva, Alexander Rosov, Lila Lifshitz, Gitit Kra, Moran Gershoni, Sergey Malitsky, Maxim Itkin, et al.placenta samples. RNA extraction of placental tissue was performed by Maxwell® RSC simplyRNA Kit (#AS1390, Promega, WI, USA) in a Maxwell® RSC Instrument (#AS4500, Promega). A total of 200 µL of each sample

(5) Suppression of interferon signaling via small molecule modulation of TFAMeLifeFebruary 6, 2026Dionisia P Sideris, Hsuan Lee, Lyndsay Olson, Kalyan Nallaparaju, Keiichiro Okuyama, Jeffrey Ciavarri, Robert Lafyatis, Mads B Larsen, Bo Lin, Irene Alfaras, et al.transcription-quantitative PCR analysis (RT-qPCR) RNA samples prepared with the Maxwell(R) RSC simplyRNA Cells (Promega, AS1390). Reverse transcription was performed with indicated primers using Applied Biosystems High-Capacity cDNA Reverse Transcription Kit

(6) Cytoplasmic poly-adenosine binding proteins modulate susceptibility of mRNAs to Pumilio-mediated decayNucleic Acids ResFebruary 5, 2026Katherine M McKenney, Carmen Hernandez-Perez, Elise B Dunshee, John M Pum, Anna J DesMarais, Robert P Connacher, Aaron C Goldstrohmat 500 × g for 5 min. RNA was purified from the cell pellet using the SimplyRNA Cells kit (Promega, AS1390) and Maxwell RSC instrument following the manufacturer’s protocol. For the on-bead DNase digestion,

(7) A titin missense variant drives atrial electrical remodeling and is associated with atrial fibrillationeLifeJanuary 22, 2026Mahmud Arif Pavel, Hanna Chen, Michael Hill, Arvind Sridhar, Miles Barney, Jaime DeSantiago, Abhinaya Baskaran, Asia Owais, Shashank Sandu, Faisal A Darbar, et al.western blotting. RNA sequencing The RNA was isolated using the Maxwell RSC simplyRNA Cells Kit (Promega AS1390) according to the instructions provided by the manufacturer. Library preparation was carried out using

(8) Unsupervised machine learning integrates genomic variants and EMR to unravel mechanisms of brain hemorrhage and epilepsy as early indicators of Alzheimer’s in down syndromeAlzheimer's Research & TherapyDecember 19, 2025Yichuan Liu, Hui-Qi Qu, Xiao Chang, Frank D. Mentch, Haijun Qiu, Kenny Nguyen, Garnet Eister, Kayleigh Ostberg, Joseph Glessner, Hakon Hakonarsondensity gradient centrifugation. Total RNA was extracted from PBMCs using the Maxwell RSC simply RNA Cells Kit (Promega, cat#AS1390) following manufacturer’s protocol. Final RNA-seq libraries were quantified with the Quant-iT dsDNA

(9) Aggressive prostate cancer is associated with pericyte dysfunctionMolecular OncologyOctober 21, 2025Anabel Martinez‐Romero, Ane Martinez‐Larrinaga, Joaquim Grego‐Bessa, Saioa Garcia‐Longarte, Hielke van Splunder, Ianire Astobiza, Amaia Ercilla, Laura Bozal‐Basterra, Isabel Mendizabal, Pilar Villacampa, et al.and quantitative polymerase chain reaction RNA was extracted using Maxwell® RSC simplyRNA cell kit (AS1390; Promega). Reverse transcription was performed from 1000 ng of RNA using the High‐Capacity cDNA Reverse

(10) Multimodal glioma immunotherapy combining TLR9-targeted STAT3 antisense oligodeoxynucleotides with PD1 immune checkpoint blockadeNeuro-OncologyOctober 14, 2025Chia-Yang Hung, Elaine Y Kang, Karol Jacek, Chunsong Yu, Xiaowei Zhang, Yicheng Zhu, Maryam Aftabizadeh, Robyn A Wong, Benham Badie, Piotr Świderski, et al.Quantitative Real-Time PCR Total RNA was extracted from cultured or in vivo grown tumor cells using Maxwell system (Promega—#AS1390) and then transcribed into cDNAs using the iScript kit (Bio-Rad—#1725064). The qPCR was carried out

(11) Implementing a trilineage differentiation in the ReproTracker assay for improved teratogenicity assessmentFrontiers in ToxicologySeptember 25, 2025José M. Horcas-Nieto, Sabine Hartvelt, Luke Flatt, Jing Fang, Esther Lam, Gaonan Zhang, Romy Van Vliet, Marleen Feliksik, Tom Zwetsloot, Connor Philippo, et al.were harvested, and total RNA was isolated using the Maxwell® RSC simply RNA Cells Kit (cat. no.: #AS1390; Promega) on a Maxwell automated purification instrument (Maxwell RSC 48; Promega). One-step Quantitative real-time PCR

(12) Protocol for monitoring mRNA translation and degradation in human cell-free lysatesSTAR ProtocolsSeptember 19, 2025Nino Schwaller, Evangelos D. Karousisassays ,, Renilla-Glo luciferase assay system,Promega,Cat. No. E2720 Maxwell RSC simplyRNA cells kit,Promega,Cat. No. AS1390 Monarch RNA cleanup kit,New England Biolabs,Cat. No. T2040L ChIP DNA Clean & Concentrator kit,Zymo Research

(13) A bioinformatics screen identifies TCF19 as an aggressiveness‐sustaining gene in prostate cancerMolecular OncologySeptember 15, 2025Amaia Ercilla, Jana R. Crespo, Saioa Garcia‐Longarte, Marta Fidalgo, Sara del Palacio, Natalia Martin‐Martin, Onintza Carlevaris, Ianire Astobiza, Sonia Fernández‐Ruiz, Marc Guiu, et al.during the follow‐up. 2.6 Real‐time quantitative RT‐PCR (qRT‐PCR) Maxwell RSC simplyRNA Cells Kit (AS1390; Promega, Madison, WI, CA) was used to isolate total RNA. Complementary DNA was produced using Maxima™

(14) PR55α subunit of protein phosphatase 2A supports KRASG12D-driven tumorigenesis that requires YAP activationOncogeneSeptember 8, 2025Christopher B. Jenkins, Alison L. Camero, Brendan T. Graff, Lepakshe S. V. Madduri, Kelly A. O’Connell, Ashley L. Hein, Lynette M. Smith, Charles A. Enke, Jixin Dong, Michael A. Hollingsworth, et al.19]. RT-PCR analysis Total RNA was isolated using the Maxwell® RSC simplyRNA Cells Kit (Promega, #AS1390) with TM411 Maxwell® RSC DNA/RNA purification instrument. mRNA expressions of human ANKRD1, CYR61, CTGF,

(15) NRCAM variant defined by microexon skipping is a targetable cell surface proteoform in high-grade gliomasCell ReportsAugust 26, 2025Priyanka Sehgal, Ammar S. Naqvi, Makenna Higgins, Jiageng Liu, Kyra Harvey, Julien Jarroux, Taewoo Kim, Berk Mankaliye, Pamela Mishra, Grace Watterson, et al.were isolated using Maxwell RSC simplyRNA Cells kit with the Maxwell RSC48 Instrument (Promega Cat#AS1390) and reverse-transcribed using SuperScript IV (Invitrogen Cat#18090010). Primer sequences used for cDNA

(16) Computational Splicing Analysis of Transcriptomic Data Reveals Sulforaphane Modulation of Alternative mRNA Splicing of DNA Repair Genes in Differentiated SH-SY5Y NeuronsInternational Journal of Molecular SciencesAugust 23, 2025Maria Lui, Luigi Chiricosta, Renato Iori, Emanuela Mazzon, Aurelio Minuti, Osvaldo ArtimagnellaSubsequently, total RNA was isolated from the cell pellet using the Maxwell® RSC simplyRNA Cells Kit (#AS1390, Promega, Madison, WI, USA) on the Maxwell® RSC automated extraction system, following the manufacturer’s

(17) Nintedanib Induces Mesenchymal-to-Epithelial Transition and Reduces Subretinal Fibrosis Through Metabolic ReprogrammingInternational journal of molecular sciencesJuly 24, 2025Hughes D, Prestle J, Zippel N, McFetridge S, Szczepan M, Neubauer H, Xu H, Chen M.software version 1.53. 4.5. RNA Extraction Total RNA using the Maxwell RSC simplyRNA Cells Kit (Cat. AS1390, Promega, Seattle, WA, USA) was isolated following manufacturer’s instructions. The concentration and

(18) Tumor Organoids Grown in Mixed-Composition Hydrogels Recapitulate the Plasticity of Pancreatic CancersGelsJuly 21, 2025Ioritz Sorzabal-Bellido, Xabier Morales, Iván Cortés-Domínguez, Maider Esparza, Lucía Grande, Pedro Castillo, Silvia Larumbe, María Monteserín, Shruthi Narayanan, Mariano Ponz-Sarvise, et al.day 7 from mature PDAC93 organoids using the Maxwell RSC SimplyRNA Kit (Promega, Madison, WIS, USA, AS1390) following the manufacturer’s protocol. Complementary DNA was synthesized using the M-MLV Reverse Transcription

(19) Dual targeting of tumoral cells and immune microenvironment by blocking the IL-33/IL1RL1 pathwayNat CommunJuly 14, 2025Denggang Fu, Hua Jiang, Alan Long, Ella Harris, Hongfen Guo, Maegan L. Capitano, John Wrangle, Joshua R. Faust, Anilkumar Gopalakrishnapillai, Santhosh Kumar Pasupuleti, et al.kit. RNA was extracted with a Promega Maxwell RSC 16 using the Maxwell RSC simplyRNA Cells kit (Cat# AS1390) according to the manufacturer’s instructions. RNA concentration was measured using a NanoDrop 8000.

(20) Efficient cell-free translation from diverse human cell typesJournal of Biological ChemistryJuly 1, 2025Jana Ziegelmüller, Nikolaos Kouvelas, Nino Schwaller, Priyanka Thambythurai, Alexander M. Hofer, Oliver Mühlemann, Evangelos D. KarousisMaxwell RSC Instrument (Promega, Cat# AS4500) and the Maxwell RSC simplyRNA Cells Kit (Promega, Cat# AS1390), following the manufacturer’s protocol. RNA concentration after extraction was determined by NanoDrop 2000 spectrophotometer

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