Maxwell RSC DNA FFPE Kit from Promega

Product Specs
ItemMaxwell RSC DNA FFPE Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1450
Quantity48 preps
Molecular Weight~85kDa
SourceCell/Tissue, Fixed Tissue
Nucleic Acid TypeGenomic DNA
Sample TypeFormalin-fixed, paraffin-embedded tissue

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Enhancing the accuracy of molecular classification of pediatric CNS tumors: a dual-classifier approach using DNA methylation profilingFrontiers in OncologyFebruary 5, 2026Esra Moosa, Rania Alanany, Shimaa Sherif, Erdener Ozer, Sukoluhle Dube, Aayesha Jabeen, Apryl Sanchez, Asma Jamil, Aisha Khalifa, Chiara Cugno, et al.after storage at -80 °C for extraction on the following day, using the Maxwell® RSC DNA FFPE Kit (#AS1450, Promega). DNA quantification was performed using the Quantus Fluorometer (E6150, Promega) with the

(2) Lactate transmission from hypoxic tumor cells promotes macrophage senescence and M2 polarization via the DNMT1-NHE7 axis to accelerate endometrial cancer progressionCell Death & DiseaseJanuary 30, 2026Shizhou Yang, Yuejiang Ma, Tingting Wu, Xiufeng Huangcomplexes were eluted, crosslinks reversed, and DNA purified using a DNA Cleanup Kit (Promega, USA, AS1450). To further evaluate NHE7 promoter methylation, methylated DNA was enriched using the EpiQuik™ Methylated

(3) High Prevalence of Potential Molecular Therapeutic Targets in Poorly Differentiated Thyroid CarcinomaEndocr PatholOctober 22, 2025Vanessa Zambelli, Giulia Orlando, Marta Fornaro, Giulia Vocino Trucco, Ida Rapa, Francesca Napoli, Susanna Cappia, Lorenzo Daniele, Simonetta Piana, Mauro Papotti, et al.selected material was extracted using Maxwell RSC DNA FFPE kit (Promega Corporation, Madison, WI, USA, CN: AS1450) and Maxwell RSC RNA FFPE kit (Promega Corporation, Madison, WI, USA, CN: 14402) according to the manufacturer’s

(4) Exploration of tongue dorsum sampling to support clinical diagnosis of leprosy patients in the Comoros: A cross-sectional studyPLOS Neglected Tropical DiseasesSeptember 17, 2025Lena Krausser, Maren Van Nieuwenhove, Nissad Attoumani, Silahi H. Grillone, Magalie Van Dyck-Lippens, Leen Rigouts, Abdallah Baco, Wirdane Abdou, Aboubacar Mzembaba, Epco Hasker, et al.mg/ml) for one hour or up to overnight at 60 °C and DNA was purified with the Maxwell RSC DNA FFPE kit (AS1450, Promega, USA) [3]. qPCR assays For qPCR assays, 2 µl DNA extract was mixed with 18 µl master mix

(5) BRCA1 promoter methylation predicts PARPi response in ovarian cancer: insights from the KOMET studyClin EpigeneticsAugust 7, 2025Léna Nougarede, Florence Hazane-Puch, Florence de Fraipont, Emmanuelle Jacquet, Marie Bidartformalin-fixed paraffin-embedded (FFPE) samples on an RSC Maxwell automate using the RSC DNA FFPE kit (#AS1450, Promega). DNA concentration was determined using a Qubit (dsDNA High Sensitivity Assay, #Q33230). BRCA

(6) NHE7 drives endometrial cancer progression by delaying senescence through cAMP/CREB/GRIN2B axis-mediated Ca²⁺ influxCommun BiolJune 5, 2025Shizhou Yang, Tao Shen, Minghua Yu, Tingting Wu, Linhua Qian, Wu Liu, Ting Wang, Xiufeng Huangfor 4 h and Core Mix at 45 °C for 1 h. DNA was purified using a DNA purification kit (Promega, USA, AS1450). qPCR was then used to amplify using the designed GRIN2B promoter primers in the ChIP products (forward

(7) Somatic PIK3R1 mutations in the iSH2 domain are accessible to PI3Kα inhibitionEMBO Molecular MedicineMay 19, 2025Gabriel Morin, Alexandre P Garneau, Nabiha Bouzakher, Louise Ségot, Antoine Fraissenon, Amélie Blondel, Florence Petit, Caroline Chopinet, Franck Mayeux, Pierre Fayoux, et al.and other reagents","Chemicals, Enzymes and other reagents" Maxwell RSC DNA FFPE Kit,Promega France,AS1450 SureSelectXT HS Custom Pannel,Agilent Technologies, Fetal Bovine Serum,Sigma,F7524 Penicillin-streptomycin,Gibco,15140122 DMEM,Gibco,11965092 Lipofectamine 3000

(8) SNUH methylation classifier for CNS tumorsClin EpigeneticsMarch 12, 2025Kwanghoon Lee, Jaemin Jeon, Jin Woo Park, Suwan Yu, Jae-Kyung Won, Kwangsoo Kim, Chul-Kee Park, Sung-Hye Parkwere delineated for macrodissection. DNA extraction was performed using the Maxwell® RSC DNA FFPE Kit (AS1450; Promega, USA), according to the manufacturer’s instructions. NGS was performed with a custom panel

(9) Universal testing in endometrial cancer in SwedenHereditary Cancer in Clinical PracticeAugust 22, 2024Emil Andersson, Anne Keränen, Kristina Lagerstedt-Robinson, Sam Ghazi, Annika Lindblom, Emma Tham, Miriam Mintsextraction from FFPE tissue was performed on 10 µm thick sample slides using the Maxwell RSC DNA FFPE Kit AS1450 (Promega) according to manufacturer's protocol on a Maxwell RSC device from Promega. DNA was then analyzed

(10) Neoadjuvant nivolumab with or without relatlimab in resectable non-small-cell lung cancer: a randomized phase 2 trialNature MedicineJune 1, 2024Martin Schuler, Kristof Cuppens, Till Plönes, Marcel Wiesweg, Bert Du Pont, Balazs Hegedus, Johannes Köster, Fabian Mairinger, Kaid Darwiche, Annette Paschen, et al.performed semiautomatically on the Maxwell purification system (Maxwell RSC DNA FFPE Kit; Promega, cat. no. AS1450) as specified by the manufacturer. DNA was eluted in 50 µl of RNase-free water and quantified fluorescently

(11) Expanding Broad Molecular Reflex Testing in Non-Small Cell Lung Cancer to Squamous HistologyCancers (Basel)February 23, 2024Martin Zacharias, Selma Konjic, Nikolaus Kratochwill, Gudrun Absenger, Angelika Terbuch, Philipp J. Jost, Robert Wurm, Jörg Lindenmann, Karl Kashofer, Franz Gollowitsch, et al.Maxwell 16 instrument (Promega, Mannheim, Germany) and the Maxwell RSC DNA FFPE Kit (Promega, Mannheim, Germany; CatNr: AS1450). After quantification of DNA via picogreen fluorescence, library preparation was performed with 10

(12) First effectiveness data of lenvatinib and pembrolizumab as first-line therapy in advanced anaplastic thyroid cancer: a retrospective cohort studyBMC Endocrine DisordersFebruary 22, 2024Dominik Soll, Philip Bischoff, Anne Frisch, Marie Jensen, Zehra Karadeniz, Martina T. Mogl, David Horst, Tobias Penzkofer, Joachim Spranger, Ulrich Keilholz, et al.areas were macrodissected. DNA was extracted using the Maxwell RSC DNA FFPE Kit (Promega, Cat. No. AS1450) and analyzed using the Oncomine Focus DNA Assay (Thermo Fisher Scientific, Cat. No. A35955). The DNA

(13) Novel cellular systems unveil mucosal melanoma initiating cells and a role for PI3K/Akt/mTOR pathway in mucosal melanoma fitnessJournal of Translational MedicineJanuary 8, 2024Matilde Monti, Luisa Benerini Gatta, Mattia Bugatti, Irene Pezzali, Sara Picinoli, Marcello Manfredi, Antonio Lavazza, Virginia Vita Vanella, Veronica De Giorgis, Lucia Zanatta, et al.Automated DNA Extraction was performed on the FFPE tissues using Maxwell® RSC DNA FFPE Kit (cat. n. AS1450, Promega, Madison, WI, USA) according to manufacturer’s protocol. Array comparative genomic hybridisation

(14) Predictive Genetic Biomarkers for the Development of Peritoneal Metastases in Colorectal CancerInt J Mol SciAugust 15, 2023Danique J. I. Heuvelings, Anne G. W. E. Wintjens, Laura Moonen, Sanne M. E. Engelen, Ignace H. J. T. de Hingh, Liselot B. Valkenburg-van Iersel, Marcel den Dulk, Jan Beckervordersandforth, Sharon G. M. Thijssen, Daphne J. G. Leunissen, et al.were extracted and isolated using a Maxwell RSC® System for Genomic DNA or RNA Extraction with a FFPE AS1450 kit and FFPE AS1440 kit, respectively (Promega, Madison, WI, USA). A blank control sample was analyzed

(15) Genomic mutation landscape of skin cancers from DNA repair-deficient xeroderma pigmentosum patientsNature CommunicationsMay 4, 2023Andrey A. Yurchenko, Fatemeh Rajabi, Tirzah Braz-Petta, Hiva Fassihi, Alan Lehmann, Chikako Nishigori, Jinxin Wang, Ismael Padioleau, Konstantin Gunbin, Leonardo Panunzi, et al.of FFPE containing a high fraction of tumor cells using Maxwell® RSC DNA FFPE Kit (Catalog number: AS1450, Promega) according to the manufacturer’s instructions. Non-tumoral DNA was extracted from FFPE blocks

(16) Genomic profiles of IDH-mutant gliomas: MYCN-amplified IDH-mutant astrocytoma had the worst prognosisScientific ReportsApril 25, 2023Kwanghoon Lee, Seong-Ik Kim, Eric Eunshik Kim, Yu-Mi Shim, Jae-Kyung Won, Chul-Kee Park, Seung Hong Choi, Hongseok Yun, Hyunju Lee, Sung-Hye Parkinstructions from serial sections of the macrodissected tumor tissue using Maxwell® RSC DNA FFPE Kit (AS1450; Promega, USA) with a tumor-targeted gene panel (FiRST brain tumor panel and FiRST pancancer panel),

(17) MRI-Based Deep Learning Tools for MGMT Promoter Methylation Detection: A Thorough EvaluationCancers (Basel)April 12, 2023Lucas Robinet, Aurore Siegfried, Margaux Roques, Ahmad Berjaoui, Elizabeth Cohen-Jonathan Moyalextraction from formalin-fixed paraffin-embedded (FFPE) tissues, MaxwellR RSC DNA FFPE Kit (Promega, AS1450, Madison, WI, USA) is used. DNA is quantified by fluorimetric assay with DNA Qubit, Broad Range Kit

(18) Platform combining statistical modeling and patient-derived organoids to facilitate personalized treatment of colorectal carcinomaJournal of Experimental & Clinical Cancer ResearchApril 3, 2023George M. Ramzy, Maxim Norkin, Thibaud Koessler, Lionel Voirol, Mathieu Tihy, Dina Hany, Thomas McKee, Frédéric Ris, Nicolas Buchs, Mylène Docquier, et al.extracted from two FFPE tissue sections of about 10 μm with Promega Maxwell® RSC DNA FFPE Kit (Promega AS1450) on the automated machine (Promega RSC). The concentration was measured using fluorometric Qubit reagents

(19) Identification of germline cancer predisposition variants in pediatric sarcoma patients from somatic tumor testingScientific ReportsFebruary 20, 2023Piedad Alba-Pavón, Lide Alaña, Miriam Gutierrez-Jimeno, Susana García-Obregón, Teresa Imízcoz, Elena Panizo, Paula González-Urdiales, Aizpea Echebarria-Barona, Ricardo Lopez Almaraz, Laura Zaldumbide, et al.quantification DNA and RNA extractions from tumor samples were carried out using Maxwell RSC DNA FFPE kit (Promega, AS1450), Maxwell RSC Tissue DNA Kit (Promega, AS1610), Maxwell RSC RNA FFPE kit (Promega, AS1440) and Maxwell

(20) Vemurafenib and Dabrafenib Downregulates RIPK4 LevelCancers (Basel)February 1, 2023Ewelina Madej, Anna A. Brożyna, Agnieszka Adamczyk, Norbert Wronski, Agnieszka Harazin-Lechowska, Anna Muzyk, Krzysztof Makuch, Michal Markiewicz, Janusz Rys, Agnieszka Wolnicka-Glubiszusing Maxwell® RSC Instrument (Promega, Walldorf, Germany). The Maxwell® RSC DNA FFPE kit (Cat. No. AS1450, Promega) was used to isolate genomic DNA using paramagnetic particles. The purity and concentration

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