ROS-Glo H2O2 Assay G8820 from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
Detection TargetH2O2
Assay Time20 minutes
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(121) Novel aroylated phenylenediamine compounds enhance antimicrobial defense and maintain airway epithelial barrier integrityScientific reportsMay 8, 2019Myszor IT, Parveen Z, Ottosson H, Bergman P, Agerberth B, Strömberg R, Gudmundsson GH.h of treatment with HO53 and HO56 (both at 75 µM) was measured using ROS-GloTM H2O2 assay (Promega, G8820) according to the manufacture’s protocol. Agarose gel electrophoresis Semi-quantitative PCR products

(122) G1/S cell cycle regulators mediate effects of circadian dysregulation on tumor growth and provide targets for timed anticancer treatmentPLoS biologyApril 1, 2019Lee Y, Lahens NF, Zhang S, Bedont J, Field JM, Sehgal A.jet-lag cells seeded in a 96-well plate, luminescence-based assays were performed using H2O2 assay kit (G8820, Promega, Madison, WI), NADP/NADPH assay kit (G9081, Promega, Madison, WI), GSH-Glutathione assay kit

(123) Tumor cells induce LAMP2a expression in tumor-associated macrophage for cancer progressionEBioMedicineFebruary 1, 2019Wang R, Liu Y, Liu L, Chen M, Wang X, Yang J, Gong Y, Ding BS, Wei Y, Wei X.treatments as described above, and measured for total ROS production by using ROS-Glo H2O2 Assay (Promega, G8820). All the outcomes were analyzed by luminescence determination in a luminometer. 2.19 Cytotoxicity

(124) Mitochondrial Dysfunction in Aged Macrophages and Lung during Primary Streptococcus pneumoniae Infection is Improved with PirfenidoneScientific reportsJanuary 30, 2019Plataki M, Cho SJ, Harris RM, Huang HR, Yun HS, Schiffer KT, Stout-Delgado HW.cell culture media) hydrogen peroxide generation was assessed using the ROS-Glo H2O2 Assay (Catalog#: G8820, Promega). Mitochondrial respiration was assessed using the MITO-ID Extracellular O2 Sensor Kit (Catalog

(125) Effects of a novel peptide Ac-SDKP in radiation-induced coronary endothelial damage and resting myocardial blood flowCardio-OncologyDecember 18, 2018Umesh C. Sharma et al.microscope). Measurement of endothelial cell reactive oxidative stress We performed ROS-Glo™ H2O2 assay (Cat No. G8820, Promega) to measure radiation-induced ROS generation in endothelial cells. The assay is based on a

(126) Leflunomide attenuates oxidative stress in fetal human lung endothelial cells via superoxide dismutase 2 and catalaseBiochemical and biophysical research communicationsSeptember 10, 2018Shrestha AK, Menon RT, Shivanna B.generation Hydrogen peroxide (H2O2) levels were quantified by the ROS-Glo™ H2O2 Assay (Promega, Madison, WI; G8820). Briefly, cells grown to 60–70% confluence on 96-well plates were treated with DMSO or leflunomide,

(127) Intracellular and Intercellular Signalling Mechanisms following DNA Damage Are Modulated By PINK1Oxidative Medicine and Cellular LongevityJune 27, 2018Mihaela Temelie, Diana Iulia Savu, Nicoleta MoisoiMeasurement H2O2 was measured using the ROS-Glo Assay Kit (Promega) following the manufacturer's instructions (G8820, Promega). The cells were seeded in 96-well plates. Three hours before the analysis, the medium was

(128) Circulating Exosomes Isolated from Septic Mice Induce Cardiovascular Hyperpermeability Through Promoting Podosome Cluster FormationShockApril 1, 2018Xingjiang Mu et al.(LDH) release assay The ROS levels in exosomes or MCECs were measured using ROS-Glo H2O2 Assay kit (G8820, Promega) according to the manufacturer's instructions. The luminescence was measured with a Tecan Microplate

(129) Temporal DNA-PK activation drives genomic instability and therapy resistance in glioma stem cellsJCI insightFebruary 8, 2018Wang Y, Xu H, Liu T, Huang M, Butter PP, Li C, Zhang L, Kao GD, Gong Y, Maity A, et al.followed by x-ray irradiation. ROS (H2O2) levels were determined using the ROS-GLO H2O2 assay (Promega, G8820) according to the manufacturer’s instructions. Luminescence was measured using a Synergy H4 Hybrid luminescent

(130) Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serumJournal of extracellular vesiclesJanuary 1, 2018Kornilov R, Puhka M, Mannerström B, Hiidenmaa H, Peltoniemi H, Siljander P, Seppänen-Kaijansinkko R, Kaur S.After 24 h and 48 h, cells were washed carefully with PBS, and the ROS-GLO H2O2 Assay (Promega Cat. no. G8820/1, lot. no. 0000264704, Fitchburg, WI, USA) was performed according to the manufacturer’s instructions.

(131) Catalase Enhances Viability of Human Chondrocytes in Culture by Reducing Reactive Oxygen Species and Counteracting Tumor Necrosis Factor-α-Induced ApoptosisCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyJanuary 1, 2018Li S, Yang X, Feng Z, Wang P, Zhu W, Cui S.System). The cells were mixed with 20 µl of the H2O2 Substrate Solution from the ROS-GloTM H2O2 Assay kit (G8820, Promega) and further incubated for 6 h in the same conditions. Cells in each well were mixed with 100

(132) Tristetraprolin inhibits mitochondrial function through suppression of α-Synuclein expression in cancer cellsOncotargetJune 27, 2017Vo MT, Choi SH, Lee JH, Hong CH, Kim JS, Lee UH, Chung HM, Lee BJ, Park JW, Cho WJ.luminescence was recorded immediately. Cellular ROS levels were measured using the ROS-Glo™ H2O2 Assay Kit (G8820, Promega) according to the manufacturer's instructions. Briefly, SHSY5Y or HeLa cells were plated on

(133) PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-NitrosylationMolecular cellMarch 16, 2017Gupta A, Anjomani-Virmouni S, Koundouros N, Dimitriadi M, Choo-Wing R, Valle A, Zheng Y, Chiu YH, Agnihotri S, Zadeh G, et al.GSH/GSSG and NOS Activity Measurements H2O2 levels were measured using the ROS-Glo H2O2 assay (Promega, G8820) according to the manufacturer’s instructions. Briefly, 1x104 shGFP control and shPARK2-expressing cells

(134) Omeprazole induces heme oxygenase-1 in fetal human pulmonary microvascular endothelial cells via hydrogen peroxide-independent Nrf2 signaling pathwayToxicology and applied pharmacologyNovember 15, 2016Patel A, Zhang S, Shrestha AK, Maturu P, Moorthy B, Shivanna B.generation Hydrogen peroxide (H2O2) levels was quantified by the ROS-Glo™ H2O2 Assay (Promega, Madison, WI; G8820) according to the manufacturer's recommendation. Briefly, cells were grown on 96-well plates to 60-70%

(135) Src Family Kinases and p38 Mitogen-Activated Protein Kinases Regulate Pluripotent Cell Differentiation in CulturePLOS ONEOctober 10, 2016Boon Siang Nicholas Tan, Joly Kwek, Chong Kum Edwin Wong, Nicholas J. Saner, Charlotte Yap, Fernando Felquer, Michael B. Morris, David K. Gardner, Peter D. Rathjen, Joy Rathjentimes. Biochemical analysis of ROS. ROS production was quantified using the ROS-Glo™ H202 Assay (Promega, G8820). MES cells were seeded at 1 x 106 cells per well into a 96 well white imaging plates (BD Falcon, 351130)

(136) Oxidative pentose phosphate pathway inhibition is a key determinant of antimalarial induced cancer cell deathOncogeneJune 2, 2016Salas E, Roy S, Marsh T, Rubin B, Debnath J.Levels of reactive oxygen species (ROS) were detected using the ROS-Glo H2O2 luminescence kit (Promega G8820) according to the manufacturer’s protocol. Immunoblotting Cells were lysed in RIPA lysis buffer plus

(137) Activation of ENaC in collecting duct cells by prorenin and its receptor PRR: involvement of Nox4-derived hydrogen peroxideAmerican Journal of Physiology-Renal PhysiologyJune 1, 2016Xiaohan Lu, Fei Wang, Mi Liu, Kevin T. Yang, Adam Nau, Donald E. Kohan, Van Reese, Russell S. Richardson, Tianxin Yangleast 15 min. Measurement of H2O2. H2O2 was measured by using the ROS-Glo H2O2 Assay kit (cat. no. G8820; Promega) according to the manufacturer’s instructions. The assay is based on a luminescent signal generated

(138) Mechanisms of growth inhibition of primary prostate epithelial cells following gamma irradiation or photodynamic therapy include senescence, necrosis, and autophagy, but not apoptosisCancer MedicineJanuary 1, 2016Fiona M. Frame, Huguette Savoie, Francesca Bryden, Francesca Giuntini, Vincent M. Mann, Matthew S. Simms, Ross W. Boyle, Norman J. Maitlandoxygen species and the end product of other ROS enzyme reactions 25, the ROS‐Glo H2O2 Assay (Promega G8820, Southampton, UK) was carried out. Cells were plated in a 96‐well plate (4000–8000 cells per well for

(139) Omeprazole does not Potentiate Acute Oxygen Toxicity in Fetal Human Pulmonary Microvascular Endothelial Cells Exposed to HyperoxiaPharmaceutica analytica actaOctober 1, 2015Patel A, Zhang S, Moorthy B, Shivanna B.generation Hydrogen peroxide (H2O2) levels was quantified by the ROSGlo™ H2O2 Assay (Promega, Madison, WI; G8820) according to the manufacturer’s recommendation. Briefly, cells were grown on 96-well plates to 60-70%

(140) Low-temperature plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cellsBritish Journal of CancerApril 28, 2015A M Hirst, M S Simms, V M Mann, N J Maitland, D O'Connell, F M Framemedia as a result of LTP treatment was detected and quantified using the ROS-Glo H2O2 assay (Cat. no. G8820, Promega, Southhampton, UK). Cells were treated with LTP, before being plated into black-walled 96-well

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ROS-Glo H2O2 Assay

ROS-Glo H2O2 Assay from Promega

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ROS-Glo H2O2 Assay from Promega

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