ROS-Glo H2O2 Assay G8820 from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
Detection TargetH2O2
Assay Time20 minutes
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(121) Circulating Exosomes Isolated from Septic Mice Induce Cardiovascular Hyperpermeability Through Promoting Podosome Cluster FormationShockApril 1, 2018Xingjiang Mu et al.assay Author Manuscript The ROS levels in exosomes or MCECs were measured using ROS-Glo H2O2 Assay kit (G8820, Promega) according to the manufacturer's instructions. The luminescence was measured with a Tecan Microplate

(122) Temporal DNA-PK activation drives genomic instability and therapy resistance in glioma stem cellsJCI insightFebruary 8, 2018Wang Y, Xu H, Liu T, Huang M, Butter PP, Li C, Zhang L, Kao GD, Gong Y, Maity A, et al.followed by x-ray irradiation. ROS (H2O2) levels were determined using the ROS-GLO H2O2 assay (Promega, G8820) according to the manufacturer’s instructions. Luminescence was measured using a Synergy H4 Hybrid luminescent

(123) Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serumJournal of extracellular vesiclesJanuary 1, 2018Kornilov R, Puhka M, Mannerström B, Hiidenmaa H, Peltoniemi H, Siljander P, Seppänen-Kaijansinkko R, Kaur S.After 24 h and 48 h, cells were washed carefully with PBS, and the ROS-GLO H2O2 Assay (Promega Cat. no. G8820/1, lot. no. 0000264704, Fitchburg, WI, USA) was performed according to the manufacturer’s instructions.

(124) Catalase Enhances Viability of Human Chondrocytes in Culture by Reducing Reactive Oxygen Species and Counteracting Tumor Necrosis Factor-α-Induced ApoptosisCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyJanuary 1, 2018Li S, Yang X, Feng Z, Wang P, Zhu W, Cui S.System). The cells were mixed with 20 µl of the H2O2 Substrate Solution from the ROS-GloTM H2O2 Assay kit (G8820, Promega) and further incubated for 6 h in the same conditions. Cells in each well were mixed with 100

(125) Tristetraprolin inhibits mitochondrial function through suppression of α-Synuclein expression in cancer cellsOncotargetJune 27, 2017Vo MT, Choi SH, Lee JH, Hong CH, Kim JS, Lee UH, Chung HM, Lee BJ, Park JW, Cho WJ.luminescence was recorded immediately. Cellular ROS levels were measured using the ROS-Glo™ H2O2 Assay Kit (G8820, Promega) according to the manufacturer's instructions. Briefly, SHSY5Y or HeLa cells were plated on

(126) PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-NitrosylationMolecular cellMarch 16, 2017Gupta A, Anjomani-Virmouni S, Koundouros N, Dimitriadi M, Choo-Wing R, Valle A, Zheng Y, Chiu YH, Agnihotri S, Zadeh G, et al.GSH/GSSG and NOS Activity Measurements H2O2 levels were measured using the ROS-Glo H2O2 assay (Promega, G8820) according to the manufacturer’s instructions. Briefly, 1x104 shGFP control and shPARK2-expressing cells

(127) Omeprazole induces heme oxygenase-1 in fetal human pulmonary microvascular endothelial cells via hydrogen peroxide-independent Nrf2 signaling pathwayToxicology and applied pharmacologyNovember 15, 2016Patel A, Zhang S, Shrestha AK, Maturu P, Moorthy B, Shivanna B.generation Hydrogen peroxide (H2O2) levels was quantified by the ROS-Glo™ H2O2 Assay (Promega, Madison, WI; G8820) according to the manufacturer's recommendation. Briefly, cells were grown on 96-well plates to 60-70%

(128) Src Family Kinases and p38 Mitogen-Activated Protein Kinases Regulate Pluripotent Cell Differentiation in CulturePLOS ONEOctober 10, 2016Boon Siang Nicholas Tan, Joly Kwek, Chong Kum Edwin Wong, Nicholas J. Saner, Charlotte Yap, Fernando Felquer, Michael B. Morris, David K. Gardner, Peter D. Rathjen, Joy Rathjentimes. Biochemical analysis of ROS. ROS production was quantified using the ROS-Glo™ H202 Assay (Promega, G8820). MES cells were seeded at 1 x 106 cells per well into a 96 well white imaging plates (BD Falcon, 351130)

(129) Oxidative pentose phosphate pathway inhibition is a key determinant of antimalarial induced cancer cell deathOncogeneJune 2, 2016Salas E, Roy S, Marsh T, Rubin B, Debnath J.Levels of reactive oxygen species (ROS) were detected using the ROS-Glo H2O2 luminescence kit (Promega G8820) according to the manufacturer’s protocol. Immunoblotting Cells were lysed in RIPA lysis buffer plus

(130) Activation of ENaC in collecting duct cells by prorenin and its receptor PRR: involvement of Nox4-derived hydrogen peroxideAmerican Journal of Physiology-Renal PhysiologyJune 1, 2016Xiaohan Lu, Fei Wang, Mi Liu, Kevin T. Yang, Adam Nau, Donald E. Kohan, Van Reese, Russell S. Richardson, Tianxin Yangleast 15 min. Measurement of H2O2. H2O2 was measured by using the ROS-Glo H2O2 Assay kit (cat. no. G8820; Promega) according to the manufacturer’s instructions. The assay is based on a luminescent signal generated

(131) Mechanisms of growth inhibition of primary prostate epithelial cells following gamma irradiation or photodynamic therapy include senescence, necrosis, and autophagy, but not apoptosisCancer MedicineJanuary 1, 2016Fiona M. Frame, Huguette Savoie, Francesca Bryden, Francesca Giuntini, Vincent M. Mann, Matthew S. Simms, Ross W. Boyle, Norman J. Maitlandoxygen species and the end product of other ROS enzyme reactions 25, the ROS‐Glo H2O2 Assay (Promega G8820, Southampton, UK) was carried out. Cells were plated in a 96‐well plate (4000–8000 cells per well for

(132) Omeprazole does not Potentiate Acute Oxygen Toxicity in Fetal Human Pulmonary Microvascular Endothelial Cells Exposed to HyperoxiaPharmaceutica analytica actaOctober 1, 2015Patel A, Zhang S, Moorthy B, Shivanna B.generation Hydrogen peroxide (H2O2) levels was quantified by the ROSGlo™ H2O2 Assay (Promega, Madison, WI; G8820) according to the manufacturer’s recommendation. Briefly, cells were grown on 96-well plates to 60-70%

(133) Low-temperature plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cellsBritish Journal of CancerApril 28, 2015A M Hirst, M S Simms, V M Mann, N J Maitland, D O'Connell, F M Framemedia as a result of LTP treatment was detected and quantified using the ROS-Glo H2O2 assay (Cat. no. G8820, Promega, Southhampton, UK). Cells were treated with LTP, before being plated into black-walled 96-well

(134) TRIB1 regulates liver regeneration by antagonizing the NRF2-mediated antioxidant responseCell death & diseaseJanuary 1, 0001"Sun X, Wang S, Miao X, Zeng S, Guo Y, Zhou A, Chen Y, Chen Y, Lv F, Fan Z, et al."double-blind fashion. Luminescence ROS assay ROS levels were measured by the ROS-Glo system (Promega, G8820) as previously described [28, 29]. Briefly, a luminescence substrate solution was added to and incubated

(135) Succinyl-CoA ligase ADP-forming subunit beta promotes stress granule assembly to regulate redox and drive cancer metastasisProceedings of the National Academy of Sciences of the United States of AmericaJanuary 1, 0001"Boese AC, Kang J, Hwang JS, Kim J, Eun K, Malin CM, Magliocca KR, Pan C, Jin L, Kang S."Mitochondria isolation kit for cultured cells (89874) was obtained from Pierce. TransIT-LT1 (MIR2304) was from MirusBio. ROS-Glo H2O2 Assay (G8820) and Caspase-Glo 3/7 Assay Systems (G8091) were obtained from Promega. EZClick Global Protein Synthesis

(136) Altered zinc homeostasis in a primary cell culture model of the retinal pigment epitheliumFrontiers in nutritionJanuary 1, 0001"Álvarez-Barrios A, Álvarez L, Artime E, García M, Lengyel I, Pereiro R, González-Iglesias H."days after renewal of culture media, using the ROS-Glo™ H2O2 Assay purchased from Promega (Cat. No. G8820). H2O2 diffused from the cells to the culture media was quantified by the luminescent signals produced

(137) PCNA inhibition enhances the cytotoxicity of β-lapachone in NQO1-Positive cancer cells by augmentation of oxidative stress-induced DNA damageCancer lettersJanuary 1, 0001"Su X, Wang J, Jiang L, Chen Y, Lu T, Mendonca MS, Huang X."during or after treatments using standard chemical assays following manufactures’ protocols. (Promega G8820, Promega G9071, Promega G7572). 2.9. PCNA knockdown by siRNA PCNA-specific siRNA ( CCUUGGCGCUAGUAUUUGAtt,

(138) Influence of 2-Nitroimidazoles in the Response of FaDu Cells to Ionizing Radiation and Hypoxia/Reoxygenation Stress"Antioxidants (Basel, Switzerland)"January 1, 0001"Rashed FB, Kate WD, Fanta M, Wiebe LI, Kumar P, Weinfeld M."used as a readout of reactive oxygen species (ROS) and were analyzed using a ROS-Glo™ H2O2 assay kit (G8820, Promega, Madison, WI, USA). Briefly, FaDu cells seeded in 96-well plates were treated as indicated

(139) "Manganese porphyrin, MnTE-2-PyP, treatment protects the prostate from radiation-induced fibrosis (RIF) by activating the NRF2 signaling pathway and enhancing SOD2 and sirtuin activity"Free radical biology & medicineJanuary 1, 0001"Shrishrimal S, Chatterjee A, Kosmacek EA, Davis PJ, McDonald JT, Oberley-Deegan RE."ROS-GLO H2O2 assay Hydrogen peroxide (H2O2) generated by MnTE-2-PyP was measured using the ROS-GLO H2O2 assay (G8820, Promega) as previously described [138]. Briefly, HPrF cells (1 × 104 cells/well in 70 μl of HPrF media)

(140) Myotonic Dystrophy type 1 cells display impaired metabolism and mitochondrial dysfunction that are reversed by metforminAgingJanuary 1, 0001"García-Puga M, Saenz-Antoñanzas A, Fernández-Torrón R, Munain AL, Matheu A."1x103 fibroblasts were plated in 96-well plates and grown for 3 days. Afterwards, ROS-Glo H2O2 Assay (G8820, Promega) was performed according to the manufacturer’s instructions. Briefly, a H2O2 substrate reacts

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ROS-Glo H2O2 Assay

ROS-Glo H2O2 Assay from Promega

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ROS-Glo H2O2 Assay from Promega

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