ROS-Glo H2O2 Assay G8820 from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
Detection TargetH2O2
Assay Time20 minutes
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(101) Dual Regulation of Tank Binding Kinase 1 by BRG1 in Hepatocytes Contributes to Reactive Oxygen Species ProductionFrontiers in cell and developmental biologyJanuary 1, 2021Lv F, Shao T, Xue Y, Miao X, Guo Y, Wang Y, Xu Y.Species Assay Quantitative measurements of intracellular ROS were performed with a ROS-Glo system (G8820, Promega). Briefly, a luminescence substrate solution was added to and incubated with cultured cells

(102) Carbomer-based adjuvant elicits CD8 T-cell immunity by inducing a distinct metabolic state in cross-presenting dendritic cellsPLoS pathogensJanuary 1, 2021Lee W, Kingstad-Bakke B, Paulson B, Larsen A, Overmyer K, Marinaik CB, Dulli K, Toy R, Vogel G, Mueller KP, et al.Deep Red in PBS for 15 minutes at 37C. For intracellular H2O2 detection, ROS-Glo H2O2 assay (Promega; G8820) was performed according to manufacturer’s instructions; luciferin activity from added H2O2 substrate

(103) Exposure to Plasma From Non-alcoholic Fatty Liver Disease Patients Affects Hepatocyte Viability, Generates Mitochondrial Dysfunction, and Modulates Pathways Involved in Fat Accumulation and InflammationFrontiers in medicineJanuary 1, 2021Grossini E, Garhwal DP, Calamita G, Romito R, Rigamonti C, Minisini R, Smirne C, Surico D, Bellan M, Pirisi M.ROS-Glo H2O2 Assay, following the manufacturer's instructions (Promega Corporation; Padova, Italy; catalog number G8820) (24, 25). The detailed description of methods is reported in Supplementary Material. The H2O2 production

(104) Nicotinamide Adenine Dinucleotide-Dependent Flavin Oxidoreductase of Mycoplasma hyopneumoniae Functions as a Potential Novel Virulence Factor and Not Only as a Metabolic EnzymeFrontiers in microbiologyJanuary 1, 2021Xie X, Hao F, Chen R, Wang J, Wei Y, Liu J, Wang H, Zhang Z, Bai Y, Shao G, et al.medium, or 80 μL PBS. Then, 20 μL of H2O2 Substrate solution from the ROS-GloTM H2O2 Assay kit (Cat No. G8820, Promega, Madison, WI, United States) was added to the cells and mixed. Thus, the final well volume

(105) Ent-Peniciherqueinone Suppresses Acetaldehyde-Induced Cytotoxicity and Oxidative Stress by Inducing ALDH and Suppressing MAPK SignalingPharmaceuticsDecember 18, 2020Oh T, Kwon M, Yu JS, Jang M, Kim GH, Kim KH, Ko SK, Ahn JS.kit (Promega, Madison, WI, USA, # V6911) and ROS-Glo™ H2 O2 assay kit (Promega, Madison, WI, USA, #G8820) were purchased from Promega (Madison, WI, USA, V6911). 2.4. Cell Viability Assay and Anti-Acetaldehyde

(106) Novel Liver X Receptor Ligand GAC0001E5 Disrupts Glutamine Metabolism and Induces Oxidative Stress in Pancreatic Cancer CellsInternational Journal of Molecular SciencesDecember 17, 2020Shivangi Srivastava, Scott Widmann, Charles Ho, Donovan Nguyen, Alexis Nguyen, Asitha Premaratne, Jan-Åke Gustafsson, Chin-Yo Linreagent. 4.6. ROS Measurement ROS levels were detected using an ROS-Glo H2 O2 Assay (Promega, Madison, WI, USA G8820). Briefly, PANC-1, BxPC-3, and MIA PaCa-2 cells (10,000 cells/well) were plated in a 96-well plate in a

(107) BrainPhys neuronal medium optimized for imaging and optogenetics in vitroNature communicationsNovember 3, 2020Zabolocki M, McCormack K, van den Hurk M, Milky B, Shoubridge AP, Adams R, Tran J, Mahadevan-Jansen A, Reineck P, Thomas J, et al.them from light. H2O2 Luminescence Measurements: H2O2 measurements were made using ROS-Glo™ (Cat. No. G8820, Promega) kits as per the manufacturer’s protocol. Upon concluding light stimulation, H2O2 substrate

(108) Effects of the Delta Opioid Receptor Agonist DADLE in a Novel Hypoxia-Reoxygenation Model on Human and Rat-Engineered Heart Tissue: A Pilot StudyBiomoleculesSeptember 11, 2020Funcke S, Werner TR, Hein M, Ulmer BM, Hansen A, Eschenhagen T, Hirt MN.The release of H2O2 into the cell culture medium was quantified by the ROS-GLO H2O2 Assay (Promega, G8820). All kits were carried out according to the manufacturer’s recommendations and analyzed with either

(109) A Novel Flow Cytometric Assay to Identify Inhibitors of RBPJ-DNA InteractionsSLAS DiscoverySeptember 1, 2020Robert J. Lake, Mark K. Haynes, Kostiantyn Dreval, Rabeya Bilkis, Larry A. Sklar, Hua-Ying FanCayman Chemical Company. H2O2 measurement H2O2 was quantified using ROS-Glo™ H2O2 assay (Promega, # G8820) according to manufacturer’s instructions. Briefly, 4 × 104 cells were transferred to white F96 MicroWell™

(110) Modeling Secondary Iron Overload Cardiomyopathy with Human Induced Pluripotent Stem Cell-Derived CardiomyocytesCell reportsJuly 14, 2020Rhee JW, Yi H, Thomas D, Lam CK, Belbachir N, Tian L, Qin X, Malisa J, Lau E, Paik DT, et al.Commercial Assays CellTiter-Glo 2.0 Promega G9242,Fisher/Promega,PRG9242 ROS-Glo™ H2O2 Assay Promega G8820,Fisher/Promega,PR-G8820 JC-10,AAT Bioquest,22800 Deposited Data,Deposited Data,Deposited Data Raw

(111) SAMHD1 Limits the Efficacy of Forodesine in Leukemia by Protecting Cells against the Cytotoxicity of dGTPCell reportsMay 12, 2020Davenne T, Klintman J, Sharma S, Rigby RE, Blest HTW, Cursi C, Bridgeman A, Dadonaite B, De Keersmaecker K, Hillmen P, et al.3/7,Promega,Cat# G6320 TaqMan Universal PCR Master Mix,Thermofisher,Cat# 4304437 ROS-Glo H2O2 assay,Promega,Cat# G8820 ,, Deposited Data,Deposited Data,Deposited Data ,, CyTOF data,This paper,FlowRepository: FR-FCM-Z2JH ,, Experimental Models

(112) A Commercial Blend of Prebiotic Fiber, Oregano, Thyme, Cinnamon Essential Oils and Yucca Schidigera (One Current™) Supplemented Feed Increased Channel Catfish Fingerling Growth and Enhanced Disease ResistanceJournal of Aquaculture, Marine Biology & EcologyJanuary 1, 2020Beth Peterman, Jessica Koppien-Fox, Lora Petrie-Hansonassay plates with four technical replicates per fish to measure ROS with the ROS-Glo H2O2 assay (Promega, G8820), LDH with the Lactate Dehydrogenase Activity Kit (Sigma, MAK0661KT) and nitrite quantification using

(113) CYP17A1 Maintains the Survival of Glioblastomas by Regulating SAR1-Mediated Endoplasmic Reticulum Health and Redox HomeostasisCancersSeptember 16, 2019Lin HY, Ko CY, Kao TJ, Yang WB, Tsai YT, Chuang JY, Hu SL, Yang PY, Lo WL, Hsu TI.Analysis, and Glutathione Peroxidase (GPx) and Glutathione Reductase (GR) Activity Analysis H2O2-Glo (#G8820) and GSH/GSSG-Glo (#V6611) assay kits were purchased from Promega Inc. and used in accordance with the

(114) Novel aroylated phenylenediamine compounds enhance antimicrobial defense and maintain airway epithelial barrier integrityScientific reportsMay 8, 2019Myszor IT, Parveen Z, Ottosson H, Bergman P, Agerberth B, Strömberg R, Gudmundsson GH.h of treatment with HO53 and HO56 (both at 75 µM) was measured using ROS-GloTM H2O2 assay (Promega, G8820) according to the manufacture’s protocol. Agarose gel electrophoresis Semi-quantitative PCR products

(115) G1/S cell cycle regulators mediate effects of circadian dysregulation on tumor growth and provide targets for timed anticancer treatmentPLoS biologyApril 1, 2019Lee Y, Lahens NF, Zhang S, Bedont J, Field JM, Sehgal A.jet-lag cells seeded in a 96-well plate, luminescence-based assays were performed using H2O2 assay kit (G8820, Promega, Madison, WI), NADP/NADPH assay kit (G9081, Promega, Madison, WI), GSH-Glutathione assay kit

(116) Tumor cells induce LAMP2a expression in tumor-associated macrophage for cancer progressionEBioMedicineFebruary 1, 2019Wang R, Liu Y, Liu L, Chen M, Wang X, Yang J, Gong Y, Ding BS, Wei Y, Wei X.treatments as described above, and measured for total ROS production by using ROS-Glo H2O2 Assay (Promega, G8820). All the outcomes were analyzed by luminescence determination in a luminometer. 2.19 Cytotoxicity

(117) Mitochondrial Dysfunction in Aged Macrophages and Lung during Primary Streptococcus pneumoniae Infection is Improved with PirfenidoneScientific reportsJanuary 30, 2019Plataki M, Cho SJ, Harris RM, Huang HR, Yun HS, Schiffer KT, Stout-Delgado HW.cell culture media) hydrogen peroxide generation was assessed using the ROS-Glo H2O2 Assay (Catalog#: G8820, Promega). Mitochondrial respiration was assessed using the MITO-ID Extracellular O2 Sensor Kit (Catalog

(118) Effects of a novel peptide Ac-SDKP in radiation-induced coronary endothelial damage and resting myocardial blood flowCardio-OncologyDecember 18, 2018Umesh C. Sharma et al.microscope). Measurement of endothelial cell reactive oxidative stress We performed ROS-Glo™ H2O2 assay (Cat No. G8820, Promega) to measure radiation-induced ROS generation in endothelial cells. The assay is based on a

(119) Leflunomide attenuates oxidative stress in fetal human lung endothelial cells via superoxide dismutase 2 and catalaseBiochemical and biophysical research communicationsSeptember 10, 2018Shrestha AK, Menon RT, Shivanna B.generation Hydrogen peroxide (H2O2) levels were quantified by the ROS-Glo™ H2O2 Assay (Promega, Madison, WI; G8820). Briefly, cells grown to 60–70% confluence on 96-well plates were treated with DMSO or leflunomide,

(120) Intracellular and Intercellular Signalling Mechanisms following DNA Damage Are Modulated By PINK1Oxidative Medicine and Cellular LongevityJune 27, 2018Mihaela Temelie, Diana Iulia Savu, Nicoleta MoisoiMeasurement H2O2 was measured using the ROS-Glo Assay Kit (Promega) following the manufacturer's instructions (G8820, Promega). The cells were seeded in 96-well plates. Three hours before the analysis, the medium was

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ROS-Glo H2O2 Assay

ROS-Glo H2O2 Assay from Promega

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ROS-Glo H2O2 Assay from Promega

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