Fig 1: Endothelial specific Stra6 mutant mice display spatial memory defect due to a reduction of retinol uptake.A–L Retinols and RBP4 measurements from dissected brain regions or serum from endothelial-specific Stra6 knockout mice (Stra6 f/f; Cdh5(PAC)-ERT2 and littermate controls (Stra6 f/f) raised on a VitA-sufficient diet (blue) or VitA-excess diet (green). Biological replicates are shown as individual data points. A–C, G–J n = 4 per genotype, D Stra6 f/f n = 7, Stra6 f/f; Cdh5(PAC)-ERT2 n = 6, E Stra6 f/f n = 6, Stra6 f/f; Cdh5(PAC)-ERT2 n = 4, F Stra6 f/f n = 5, Stra6 f/f; Cdh5(PAC)-ERT2 n = 5, K Stra6 f/f n = 8, Stra6 f/f; Cdh5(PAC)-ERT2 n = 8, L Stra6 f/f n = 5, Stra6 f/f; Cdh5(PAC)-ERT2 n = 4. Statistics and p-values (A–L): unpaired two-tailed t-tests: A P = 0.17, B ***P = 0.0010, C *P = 0.012, D P = 0.55, E P = 0.38, F P = 0.72, G *P = 0.020, H P = 0.84, I P = 0.90, J P = 0.15, K P = 0.61, L P = 0.47. M Discrimination index for the novel location recognition task in endothelial-specific Stra6 knockout mice (Stra6 f/f; Cdh5(PAC)-ERT2, n = 23) and littermate controls (Stra6 f/f, n = 20) raised on a VitA-sufficient diet (blue). N Latency to fall in endothelial-specific Stra6 knockout (n = 16) and littermate control mice (n = 10) raised on VitA-sufficient diet (blue). O Discrimination index for the novel object recognition task in in endothelial-specific Stra6 knockout mice (n = 9) and littermate controls (n = 9) fed on VitA-sufficient diet. P Discrimination index for the novel location recognition task in endothelial-specific Stra6 knockout mice (n = 21) and littermate controls (n = 24) raised on a VitA-excess diet (green). Q Latency to fall in endothelial-specific Stra6 knockout (n = 7) and littermate control mice (n = 11) raised on VitA-excess diet (green). Statistics and p-values (M–Q): unpaired two-tailed t-tests: M ***P = 0.0008, N Day 1 P = 0.28, Day 2 P = 0.27, Day 3 P = 0.54, Day 4 P = 0.42, Day 5 P = 0.58, O P = 0.96, P P = 0.15, Q Day 1 P = 0.84, Day 2 P = 0.99, Day 3 P = 0.86, Day 4 P = 0.64, Day 5 P = 0.94. A–L, N, Q Error bars represent SEM. M, O, P Dashed lines indicate median and dotted lines indicate first and third quartiles. Source data are provided as a Source Data file.