Protease Inhibitor Cocktail ab65621 from Abcam

Product Specs
ItemProtease Inhibitor Cocktail
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65621
Quantity1 Kit
TypeInhibitor

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Investigation of ELF5, KIF18A, NPTX1 and COL23A1 genes in the pathophysiology of indirect inguinal hernia in childrenPediatr Surg IntApril 2, 2026Ercan Genc, Tugay Tartar, Ebru Onalan, Unal Bakal, Mehmet Sarac, Tugce Kaymaz, Ahmet Kazezadding 450 µl PBS (phosphate buffered saline) and 10 µl Protease Inhibitor Cocktail (Abcam, Cat No: ab65621). After homogenization, the samples were centrifuged at 4,000 rpm for 10 min at 4 °C (Beckman Coulter

(2) Differential chromatin looping regulated by two GA-binding transcription factors creates an X-specific chromatin environment for dosage compensationbioRxivMarch 17, 2026Joseph Aguilera, Kaitlyn Cortez, Luna C. Segarra Segarra Alonzo, Melissa Aldana, Angelica Aragon, Claire Gray, Morgan Woodman-Sousa, Kathryn J. Grive, Mukulika Ray, Erica LarschanBuffer (Cell Signaling Technology, 9803) supplemented with 1 mM protease inhibitor cocktail (Abcam, ab65621). Protein concentrations were determined using the DC Protein Assay (Bio-Rad, 5000116). Equal amounts

(3) Characterizing ghrelin’s role in dysregulating GLP-1-mediated function and promoting the development of alcohol-associated liver diseaseBiomedicine & PharmacotherapyFebruary 1, 2026Sundararajan Mahalingam, Ramesh Bellamkonda, Kusum K. Kharbanda, Ojeshvi Ethiraj, Nagasundaram Nagarajan, Chittibabu Guda, Kanika Sharma, Micah B. Schott, Carol A. Casey, Lorenzo Leggio, et al.0.5% sodium deoxycholate, and 0.1% SDS, pH 8) supplemented with 1% protease inhibitor cocktail (Abcam, ab65621) to prepare lysates. Further, cell lysates were subjected to co-immunoprecipitation by using Dynabeads™ Protein G Immunoprecipitation Kit

(4) CYLD Limits Neutrophil-Driven Psoriatic InflammationInflammationJanuary 20, 2026Zhenzong Fa, Zeping Huang, Yi Shang, Yang Yang, Qun Xie, Runping YangSDS, 1% Triton X-100, 0.2% sodium deoxycholate, 50 mM NaF, 1×proteinase inhibitor cocktail (Abcam, ab65621), 2 mM PMSF, and 5 mM NEM. The lysates were cleared by centrifugation for 30 min at 12,000×g quantified

(5) Tau-targeting active immunotherapy slows progression and reduces pathology in mouse models of tauopathyBrain pathology (Zurich, Switzerland)December 4, 2025Brown CM, Brooks JK, Kelly L, Vroom MM, Longo M, Dodart JC, Carare R, Boyd JD.buffer (50 mM Tris–HCl (pH 7.4), 150 mM NaCl, 1% Triton X‐100, and protease inhibitor cocktail (Abcam # ab65621)). Brain homogenates were centrifuged at 750 × g for 5 min to pellet cell debris and nuclear proteins,

(6) Combined effects of gabapentin with verapamil and metoprolol on cardiovascular function of anesthetized male ratsEuropean Journal of PharmacologySeptember 5, 2025Sarah Pribil Pribil Pardun, Spencer Duff, Ian Papenfus, Will Bruening, Yulong Li, Gurudutt Pendyala, Lie Gao(50 mM TrisHCl, 195 mM NaCl, 2 mM EDTA, 1 % NP-40, 0.1 % SDS) with 1 % protease inhibitor cocktail (ab65621 Abcam, Cambridge, UK) and then centrifuged at 20,000×g × 20 min at 4 °C. The protein concentration was

(7) Early olfactory dysfunction in experimental autoimmune encephalomyelitis reflects transient brain barrier breach and initiation of neuroinflammation in the olfactory bulbFrontiers in Cellular NeuroscienceSeptember 3, 2025Andjela Stekic, Milorad Dragic, Ivana Stevanovic, Marina Zaric Kontic, Marija Adzic Bukvic, Sanja Dacic, Milica Ninkovic, Nadezda Nedeljkovicbuffer (0.32 M sucrose in 5 mM HEPES pH 7.4) supplemented with 0.5% w/v protease inhibitor cocktail (ab65621, Abcam, United Kingdom), in a volume ratio of 1 mg tissue/9 μL isolation buffer. Crude nuclear (P1)

(8) Mechanistic insights into Aloin-mediated regulation of STAT3/SLC7A11 signaling in head and neck squamous cell carcinomaCytotechnologyJuly 3, 2025Li-Jian Wang, Jun WangRIPA buffer (Cat. No.: ab156034, Abcam, USA) supplemented with Protease Inhibitor Cocktail (Cat. No.: ab65621) and Phosphatase Inhibitor Cocktail (Cat. No.: ab201112). Protein concentrations were determined using

(9) ZNFX1 suppresses apoptosis-associated mRNA stability in cardiomyocyte to protect against myocardial infarctionRedox BiolJune 10, 2025Yang Shi, Zeqi Sun, Yongchao Chen, Yanli Xie, Chen Chen, Han Lou, Jan Mohammad Omar, Lei Wang, Ling Liu, Heng Liu, et al.Heart tissue/cell lysates were produced in RIPA buffer supplemented with Protease inhibitor (Abcam, ab65621). Subsequently, samples were boiled in 6 × loading buffer (Beyotime, P0015F) for 5 min at 99 °C. SDS-PAGE

(10) Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse modelFront Cell Dev BiolMarch 6, 2025Wenyu Zhuo, Mingyang Zhang, Jiajia Tan, Yang Gao, Yan Wang, Nana Wang, Jin Ma, Jiaying Zhang, Zhiheng Liu, Haitao Lv, et al.lysed with RIPA lysis buffer (Solarbio, R0010) supplemented with a protease inhibitor cocktail (Abcam, ab65621). The lactylation of proteins in the lysate was analyzed by Western blotting using an anti-L-lactyl

(11) NF-κB associated markers of prognosis in early and metastatic triple negative breast cancerBreast Cancer ResearchDecember 2, 2024Payton De La Cruz, Julia McAdams, Melanie Morales Aquino, Aileen I. Fernandez, Andrew Elliott, Maryam Lustberg, Christoph Schorl, Jennifer R. Ribeiro, Nicole E. Jamesextracted using Cell Lysis Buffer (Cell Signaling 9803) with 1 mM of protease inhibitor cocktail (AbCam, ab65621). All protein samples were then centrifuged at 13,000 RPM for 20 min at 4 degrees C. Total protein concentration

(12) FGF12 Positively Regulates Keratinocyte Proliferation by Stabilizing MDM2 and Inhibiting p53 Activity in PsoriasisAdvanced ScienceOctober 1, 2024Nan Wang, Xiejun Xu, Fangqian Guan, Yifan Lin, Yizhou Ye, Jie Zhou, Jianjun Feng, Sihang Li, Junbo Ye, Zhouhao Tang, et al.lysis buffer (Thermo Fisher Scientific) containing Protease and Phosphatase Inhibitor Cocktail (Abcam, ab65621) was utilized to extract total proteins from fresh skin tissues and cell samples. The protein concentrations

(13) Anticancer Activity of Imidazolyl Gold(I/III) Compounds in Non-Small Cell Lung Cancer Cell LinesPharmaceuticals (Basel)August 28, 2024Rossana Galassi, Nicola Sargentoni, Sofia Renzi, Lorenzo Luciani, Caterina Bartolacci, Prasad Pattabhi, Cristina Andreani, Stefania Pucciarelliwere bought from Supelco (Merck). The TrxR assay Kit (ab83463) and the Proteinase Inhibitor Cocktail (ab65621) were purchased from Abcam (Waltham, MA, USA). The plasmid pET17b encoding the cDNA for the hDHFR was

(14) Impaired olfactory performance and anxiety-like behavior in a rat model of multiple sclerosis are associated with enhanced adenosine signaling in the olfactory bulb via A1R, A2BR, and A3RFrontiers in Cellular NeuroscienceJuly 30, 2024Andjela Stekic, Milorad Dragic, Jelena Stanojevic, Marina Zaric Kontic, Ivana Stevanovic, Milica Zeljkovic Jovanovic, Katarina Mihajlovic, Nadezda Nedeljkovicbuffer (0.32 M sucrose in 5 mM HEPES pH 7.4) supplemented with 0.5% w/v protease inhibitor cocktail (ab65621, Abcam, UK). The crude nuclear fraction (P1) was pelleted by centrifugation at 1,000 × g for 10 min,

(15) Pro-Oxidant Auranofin and Glutathione-Depleting Combination Unveils Synergistic Lethality in Glioblastoma Cells with Aberrant Epidermal Growth Factor Receptor ExpressionCancers (Basel)June 25, 2024Elvis Martinez-Jaramillo, Fatemeh Jamali, Farah H. Abdalbari, Bassam Abdulkarim, Bertrand J. Jean-Claude, Carlos M. Telleria, Siham Sabrihomogenized on ice with 100 μL of cold 1X assay buffer containing 1X protease inhibitor cocktail (Cat. No. ab65621, Abcam) and 50 μg of protein was used for each analysis. One unit of TrxR was considered the amount

(16) All-Trans Retinoic Acid-Induced Cell Surface Heat Shock Protein 90 Mediates Tau Protein Internalization and Degradation in Human MicrogliaMolecular NeurobiologyJune 20, 2024Ngoc Lan Nguyen, Thi Xoan Hoang, Jae Young KimJapan). Western Blot Cells were lysed in a 1% Triton-X (Cat#H5141) (Biocompare) and protease inhibitor (Cat# ab65621) (Abcam) mixture. Protein concentrations were determined using the Bradford Assay Kit (Bio-Rad). Proteins

(17) Locally applied heat stress during exercise training may promote adaptations to mitochondrial enzyme activities in skeletal musclePflügers Archiv - European Journal of PhysiologyJune 1, 2024Ed Maunder, Andrew King, Jeffrey A. Rothschild, Matthew J. Brick, Warren B. Leigh, Christopher P. Hedges, Troy L. Merry, Andrew E. Kildingcold PBS and then suspended in RIPA buffer (20 µL.mg−1 muscle) made with protease inhibitor cocktail (ab65621, Abcam®, 20 μL.mL−1) and PMSF (ab141032, Abcam®, 5 μL.mL−1), and PBS (20 μL.mg−1). Samples were then

(18) Enhanced amphiregulin exposure promotes modulation of the high grade serous ovarian cancer tumor immune microenvironmentFrontiers in PharmacologyMay 20, 2024Jasmine Ebott, Julia McAdams, Chloe Kim, Corrine Jansen, Morgan Woodman, Payton De La Cruz, Christoph Schrol, Jennifer Ribeiro, Nicole Jamespellets using Cell Lysis Buffer (Cell Signaling 9803) with 1 mM of a protease inhibitor cocktail (AbCam, ab65621). Concentrations for all extracted proteins were determined by the DC Protein Assay (Bio-Rad Laboratories,

(19) Role of ghrelin hormone in the development of alcohol-associated liver diseaseBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapieMay 1, 2024Mahalingam S, Bellamkonda R, Kharbanda KK, Arumugam MK, Kumar V, Casey CA, Leggio L, Rasineni K.1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS, pH 8) with 1% protease inhibitor cocktail (Abcam, ab65621). The protein concentration was estimated in each sample using BCA Protein Assay Kit (Pierce). An amount

(20) Cellular Pre-Adaptation to the High O2 Concentration Used in Standard Cell Culture Confers Resistance to Subsequent H2O2-Induced Cell DeathAntioxidants (Basel)February 22, 2024Jack B. Jordan, Miranda J. Smallwood, Gary R. Smerdon, Paul G. WinyardµM bestatin, 0.3 µM aprotinin, and 0.5 mM 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride; #ab65621; Abcam, Cambridge, UK). The cells were left to swell on ice for 15 min with intermittent mixing every

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Protease Inhibitor Cocktail from Abcam

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