E2-Ubiquitin Conjugation Kit ab139472 from Abcam

Product Specs
ItemE2-Ubiquitin Conjugation Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab139472
Sample TypePurified protein
Quantity50 Test

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Abcam

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Cambridge CB2 0AX

United Kingdom

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+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Multiple directed mutagenesis reduces enzymatic activity and antibody recognition of the African Swine Fever Virus E2 ubiquitin-conjugating protein (ASFV-pI215L)Emerging Microbes & InfectionsJanuary 23, 2026Nuno Jordão, Ana Catarina Urbano, Fernando Boinas, Carlos Martins, Fernando FerreiraASFV-pI215L was adapted from previous work [14], using an E2-Ubiquitin Conjugation Kit (Abcam, ref. ab139472). Briefly, 2 µL of ubiquitination buffer, 2 µL of biotinylated ubiquitin solution, 1 µL of E1 solution

(2) SDCBP/Syntenin-1 stabilizes BACH1 by disassembling the SCF(FBXO22)-BACH1 complex in triple-negative breast cancerThe EMBO journalJune 1, 2025Tran PL, Kim O, Hwangbo C, Kim HJ, Kim YM, Lee JH.Pulldown,Invitrogen,Cat#10103D Ubiquitylation Assay kit,Abcam,Cat#ab139467 E2-Ubiquitin Conjugation Kit,Abcam,Cat#ab139472 Heme assay kit,Abcam,Cat#ab272534 Seahorse XFe35 V7 PS kit,Agilent Technologies,Cat#100777-004 "Dako

(3) CDK1-cyclin-B1-induced kindlin degradation drives focal adhesion disassembly at mitotic entryNature cell biologyMay 1, 2022Chen NP, Aretz J, Fässler R.recombinant CDK1–cyclin B1 as described above was incubated in 50 µl ubiquitination buffer (Abcam, ab139472) containing 2.5 µM ubiquitin (WT ab139472 or K48R ab207972, Abcam), 100 nM E1, 2.5 µM E2 (Abcam, ab139472),

(4) BRCA1-BARD1 regulates transcription through modulating topoisomerase IIβOpen biologyOctober 1, 2021Bunch H, Jeong J, Kang K, Jo DS, Cong ATQ, Kim D, Kim D, Cho DH, Lee YM, Chen BPC, et al.Santa Cruz biotechnology). For E2 ubiquitin-conjugating enzyme screening, E2 enzymes were purchased (ab139472, Abcam) and the experiments were performed according to the manufacturer's recommendations. Each of

(5) African swine fever virus encodes for an E2-ubiquitin conjugating enzyme that is mono- and di-ubiquitinated and required for viral replication cycleScientific ReportsDecember 1, 2018Ferdinando B. Freitas, Gonçalo Frouco, Carlos Martins, Fernando Ferreirasimilar to an E2 ubiquitin conjugating enzyme, a commercial kit was used (E2-Ubiquitin Conjugation Kit, ab139472, Abcam) and the manufacturer’s instructions were followed. Reactions were performed in a 50 µl mixture

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E2-Ubiquitin Conjugation Kit from Abcam

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E2-Ubiquitin Conjugation Kit from Abcam

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