Fig 1: MβCD/IL-mix-induced AD-like phenotypes and expression of related genes in 3D epidermal models. (a) Histological sections of 3D models treated with different concentrations of MβCD/IL-4/IL-13/IL-25. 25: MβCD (7.5 mM), IL-4 (25 ng/mL), IL-13 (25 ng/mL), IL-25 (10 ng/mL); 50: MβCD (7.5 mM), IL-4 (50 ng/mL), IL-13 (50 ng/mL), IL-25 (20 ng/mL); 100: MβCD (7.5 mM), IL-4 (100 ng/mL), IL-13 (100 ng/mL), IL-25 (40 ng/mL). Scale bar 50 µm (n=3). (b) qPCR analysis (n=4) (*p < 0.05, **p < 0.01, ****p < 0.0001, n.s., not significant). (c) Cytokine array TSLP and IL-1α analysis (n=3) (**p < 0.01, ****p < 0.0001).
Fig 2: AAE partially improved the AD-related cytokine expression in AD-like skin equivalent model. (a) Cytokine array TSLP, IL-1α, IL-6 and IL-8 analysis (n=3) (**p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not significant). (b and c) Protein expressions of IL-1α and TSLP determined by Western blot (n=4) (*p < 0.05, **p < 0.01, ***p < 0.001).
Fig 3: MβCD/IL-mix-induced AD-like phenotypes and expression of related genes in 3D epidermal models. (a) Histological sections of 3D models treated with different concentrations of MβCD/IL-4/IL-13/IL-25. 25: MβCD (7.5 mM), IL-4 (25 ng/mL), IL-13 (25 ng/mL), IL-25 (10 ng/mL); 50: MβCD (7.5 mM), IL-4 (50 ng/mL), IL-13 (50 ng/mL), IL-25 (20 ng/mL); 100: MβCD (7.5 mM), IL-4 (100 ng/mL), IL-13 (100 ng/mL), IL-25 (40 ng/mL). Scale bar 50 µm (n=3). (b) qPCR analysis (n=4) (*p < 0.05, **p < 0.01, ****p < 0.0001, n.s., not significant). (c) Cytokine array TSLP and IL-1α analysis (n=3) (**p < 0.01, ****p < 0.0001).
Fig 4: TSLP concentration reported in pg/mL corresponding to the selected study sites. Each subject is represented by a dot in the plot. Median values analyzed by Kruskal–Wallis test with Dunn’s test for multiple comparisons. Error bars are first and third quartiles. ** p < 0.01 and *** p < 0.001. Abbreviations: COL: Colima—Villa de Alvarez; TEC: Tecoman; ARM: Armeria; MAN: Manzanillo; CAM: Campos; QU: Queseria; MIN: Minatitlán.
Fig 5: AAE partially improved the AD-related cytokine expression in AD-like skin equivalent model. (a) Cytokine array TSLP, IL-1α, IL-6 and IL-8 analysis (n=3) (**p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not significant). (b and c) Protein expressions of IL-1α and TSLP determined by Western blot (n=4) (*p < 0.05, **p < 0.01, ***p < 0.001).
Supplier Page from Abcam for Human TSLP ELISA Kit