Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (16 Samples) 20040525 from Illumina

Product Specs
ItemIllumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (16 Samples)
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog Number20040525
ApplicationsTotal RNA-Seq, Whole-transcriptome sequencing
ReactivityHuman, Mouse, Rat, Bacteria
TypeRNA Library Prep Kit
Quantity16 Samples

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Illumina

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San Diego, CA 92122

United States

Phone: 858.202.4500

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Website: http://illumina.com

(1) Selective targeting of a histone-like silencer Sfx to the R6K conjugal transfer operonNucleic Acids ResJune 10, 2026Bing Wang, Ritika Gupta, Nathan Blaine, Barbare Khitiri, Catherine Jordan, Natalia Molotievskiy, David Dunlap, Laura Finzi, Irina ArtsimovitchSamples were barcoded using IDT for Illumina UD Indexes. Stranded Total RNA Library Prep (Illumina, cat#20040525) was used for RNA samples following Illumina’s protocol. Library concentration was measured using a

(2) Prognostic impact of circulating vitamin D and genetic variants in the vitamin D pathway in glioblastomaNeurooncol AdvJune 8, 2026Mariangela Morelli, Francesca Di Lorenzo, Francesca Marchetto, Michele Menicagli, Sara Franceschi, Manuel Giacomarra, Carlo Gambacciani, Francesco Pieri, Francesco Pasqualetti, Paolo Aretini, et al.100 ng of total RNA using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus kit (Illumina; cat#20040525), which enables depletion of ribosomal RNA and retention of coding and non-coding transcripts, following

(3) Insights into the Mechanism Underlying the Alkane Dehydrogenation Capability of Rhodococcus sp. KSM-B-3MMicroorganismsJune 2, 2026Nurit Edri, Keren Buhnik-Rosenblau, Anthony Cohen, Shlomit Hakim, Fabian Glaser, Guy Horev, Jeffrey Bruffaerts, Ilan Marek, Yechezkel Kashilibraries were prepared using the Illumina Stranded Total RNA Prep Ligation RiboZero (Illumina, cat no. 20040525, San Diego, CA, USA) according to the manufacturer’s protocol. Libraries were sequenced on the Illumina

(4) Stage-specific remodeling of wingless-related integration sites (WNT) signaling during oocyte-to-embryo transition in pigsJournal of Animal ScienceMay 9, 2026Carolina G Cabrera-Gomez, David Gascon-Collado, Paula Blanco-Cueto, Belen Pequeño, Heriberto Rodriguez-Martinez, Cristina A Martinez-Serranoprepared using the Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit (Illumina, USA, Cat. # 20040525), which enables depletion of ribosomal RNA and preserves strand information. Library preparation was

(5) Cross-neutralization and antigenic characterization of simian and equine group A rotavirusesJournal of VirologyApril 21, 2026Shalini Soni, Lianne G. Eertink, Ni Shuisong, Alan Loynachan, Samantha M. Barnum, Emma N. Adam, Michael A. Kennedy, Dan Wang, Feng Limachine and Illumina reagents: Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (Illumina, 20040525), and MiSeq Reagent Kit version 3 (600 cycles; Illumina, MS-102-3003). RNA preparation was done according

(6) Neoadjuvant palbociclib and endocrine therapy versus chemotherapy in ER + /HER2- breast cancer: a randomized phase II trialNat CommunApril 8, 2026Alexios Matikas, Evangelos Tzoras, Michail Sarafidis, Emmanouil G. Sifakis, Judith Bjöhle, Elin Barnekow, Sara Margolin, Erika Isaksson-Friman, Luisa Edman Kessler, Athanasios Zouzos, et al.using the Illumina Stranded Total RNA library preparation kit with Ribo-Zero Plus treatment (cat # 20040525/20040529, Illumina Inc.). Unique dual indexes (cat # 20040553/20040554, Illumina Inc.) were used. The

(7) Chromatin dynamics identifies 78 genes at loci associated with elevated intraocular pressure and primary open-angle glaucomabioRxivMarch 19, 2026Nivedita Singh, Zachary Batz, Jayshree Advani, Milton A. English, Rupalatha Maddala, Ponugoti Vasantha Rao, Anand Swaroopusing the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit (San Diego, CA, USA; cat# 20040525). Libraries were sequenced on an Illumina NextSeq 2000 platform and reads were trimmed to remove low

(8) Reduced vascular leakage correlates with breast carcinoma T regulatory cell infiltration but not with metastatic propensityMolecular OncologyMarch 1, 2026Liqun He, Chiara Testini, Neda Hekmati, Altea Bonello, Aglaia Schiza, Emmanuel Nwadozi, Mia Phillipson, Carina Strell, Michael WelshRNA using the Illumina Stranded Total RNA library preparation kit with Ribo‐Zero Plus treatment (cat# 20040525/20040529, Illumina Inc., San Diego, CA, USA). Unique dual indexes (cat# 20040553/20040554, Illumina

(9) Quantitative mapping of pseudouridines in bacterial RNANature CommunicationsFebruary 26, 2026Shikha Sharma, Brendan Woodworth, Bin Yang, Ning Duan, Mannuku Pheko, Niki Moutsopoulos, Akintunde Emiolahalves). Ribosomal RNA was depleted in both halves using Ribo-Zero Plus rRNA Depletion Kit (Illumina, #20040525) followed by fragmentation of all samples by addition of 0.9 μl of fragmentation reagents (Thermo Fisher Scientific

(10) Formyl peptide receptor 2 activation by MR-39 inhibits glioblastoma cell proliferation and invasiveness through suppression of multiple oncogenic pathwaysJournal of Translational MedicineFebruary 6, 2026Maria Grazia Ferraro, Marco Bocchetti, Marianna Affinito, Ercolano Giuseppe, Kardelen Dalim Filiz, Luisa Speranza, Enza Lacivita, Marcello Leopoldo, Marianna Crispino, Anna Ceccarelli, et al.biological replicate, we used the Illumina Stranded Total RNA prep. Ligation with Ribo-Zero Plus (#20040525) according to the manufacturer’s protocol, starting with 500 ng RNA per sample (9.7 average RIN). After

(11) The protective effect of maternal electroacupuncture on prenatal nicotine exposure-induced intrauterine growth restriction in rats by improving placental angiogenesisChinese MedicineJanuary 23, 2026Xiaoxuan Liu, Bo Ji, Yitian Liu, Liyu Liu, Yang Fang, Shiqi Guo, Ling Zhang, Tingting Guo, Reiko Sakurai, Virender K. RehanRNA-seq transcriptome library was prepared using Illumina® Stranded mRNA Prep, Ligation (catalog#: 20040525, San Diego, CA), using 1 μg of total RNA. Messenger RNA was isolated according to polyA selection method

(12) NAT10 promotes cancer metastasis by modulating p300/CBP activity through chromatin-associated tRNAMol CellDecember 18, 2025Ruhul Amin, Ngoc-Han Ha, Tinghu Qiu, Ronald Holewinski, Khiem C. Lam, Amélie Daugherty-Lopès, Huaitian Liu, Andy D. Tran, Maxwell P. Lee, Thorkell Andresson, et al.,Integrated DNA Technologies,Cat# 10009877 Illumina Stranded Total RNA-seq Library Prep Kit with RiboZero Plus,Illumina,Cat# 20040525 NucleoBond Xtra BAC kit,Takara Bio,Cat# 740436.25 Chromatide Alexa Fluor 488-5-dUTP kit,Thermo Fisher

(13) Metabolic profiling of therapy-induced senescent cancer cells via TPEF, MALDI-MS, and RNA-sequencingScientific ReportsDecember 17, 2025Silvia Ghislanzoni, Federica Padelli, Matteo Niero, Alessia Bertolotti, Antonino Belfiore, Simone Torelli, Arianna Bresci, Andrea Masella, Silvia Betti, Dario Polli, et al.Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero™ Plus kit (Illumina, San Diego, CA, USA, Cat# 20040525), following the manufacturer’s protocol. Briefly, libraries were generated from 100 ng of total RNA,

(14) Distinct transcriptomic and epigenomic responses of mature oligodendrocytes during disease progression in a mouse model of multiple sclerosisNature neuroscienceDecember 1, 2025Zheng C, Hervé B, Meijer M, Rubio Rodríguez-Kirby LA, Guerreiro Cacais AO, Kukanja P, Kabbe M, Jimenez-Beristain T, Olsson T, Agirre E, et al.RNA-seq libraries were prepared using Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (Illumina, 20040525), according to the manufacturer’s protocol. Libraries were sequenced on an Illumina NovaSeq X, with

(15) A delivered DNase toxin creates population heterogeneity through transient intoxication of siblingsmBioNovember 12, 2025Hanna Eriksson, Susan Schlegel, Sanna KoskiniemiRNA using the Illumina Stranded Total RNA library preparation kit with Ribo-Zero Plus treatment (cat# 20040525/20040529, Illumina Inc.). Unique dual indexes (cat# 20040553/20040554, Illumina Inc.) were used. The

(16) Chemical modulation of gut bacterial metabolism induces colanic acid and extends the lifespan of nematode and mammalian hostsPLOS BiologyNovember 11, 2025Guo Hu, Marzia Savini, Matthew Brandon Cooke, Xin Wei, Dinghuan Deng, Shihong M. Gao, Ruyue Alps Xia, Youchen Guan, Alice X. Wen, Xin Yu, et al.libraries were prepared for Illumina sequencing using the Illumina stranded total RNA prep kit (Catalog No. 20040525). Libraries were sequenced on an Illumina NextSeq 550 platform with 2 × 37-cycle paired-end reads. Bcl2fastq2

(17) MYOD represses gene expression from non-E-box motifsGenes & developmentNovember 3, 2025Nicoletti C, Massenet J, Pintado-Urbanc AP, Connor LJ, Nicolau M, Sundar S, Xu M, Schmitt A, Zhang W, Fang Z, et al.using the Illumina stranded total RNA preparation, as well as ligation with Ribo-Zero Plus (Illumina 20040525). ERCC RNA spike-in mix was added at the start of the protocol according to the manufacturer's instruction

(18) Differential tissue tropism and transmission efficiency of two dominant influenza D clades with overlapping but distinct receptor binding fine specificities in ferretsPLOS PathogensSeptember 15, 2025Tirth Uprety, Chithra C. Sreenivasan, Jieshi Yu, Miaoyun Zhao, Runxia Liu, Hai Yu, Ahsan Naveed, Lianne G. Eertink, Shalini Soni, Rebecca E. Ruby, et al.generated using Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit (Illumina; catalog number 20040525). Libraries were sequenced on MiSeq platform using V2 chemistry kits. Adapter trimming was performed

(19) Promoter- and enhancer-dependent cohesin loading initiates chromosome looping to fold Tcrb loci for long-range recombinationCell ReportsAugust 26, 2025Brittney M. Allyn, Katharina E. Hayer, Clement Oyeniran, Vincent Nganga, Kyutae Lee, Ahmet Sacan, Eugene M. Oltz, Craig H. Bassingthe isolation of nascent RNA, the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Kit (20040525) with the IDT for Illumina RNA UD Indexes Set A (20040529) were used for library prep minus the ribosomal

(20) Multi-omics characterization of a lytic phage targeting Listeria monocytogenesmSystemsJuly 22, 2025Stevan Cucić, Leena Putzeys, Maarten Boon, Dion Lepp, Rob Lavigne, Cezar M. Khursigara, Hany AnanyRNA samples using an Illumina Stranded Total RNA prep, Ligation with Ribo-Zero Plus kit (Illumina; 20040525) according to the manufacturer’s instructions. Sequencing was performed at Genomics Core Leuven. Illumina-based

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Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (16 Samples) from Illumina

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Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (16 Samples) from Illumina

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