Nextera XT DNA Library Preparation Kit (96 Samples) FC-131-1096 from Illumina

Product Specs
ItemNextera XT DNA Library Preparation Kit (96 Samples)
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog NumberFC-131-1096
ApplicationsAmplicon sequencing, WGS, De novo sequencing, Metagenomics
ReactivityAny
TypeDNA Library Prep Kit
Quantity96 Samples

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Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) Children’s gut microbiota predicts the efficacy of obesity treatmentGut MicrobesDecember 31, 2026Mireia Alcázar, Verónica Luque, Natalia Ferré, Judit Muñoz-Hernando, Mariona Gispert-Llauradó, Ricardo Closa-Monasterolo, Albert Feliu, Gemma Castillejo, Joaquín Escribanowas used to initiate the protocol. The multiplexing step was performed with a Nextera XT Index Kit (FC-131-1096) (Illumina, San Diego, CA, USA). One μl of the PCR product was run on a Bioanalyzer DNA 1000 chip to

(2) Multiple Reassortments, Limited Geographic Spread, and Comparable Pathogenicity of Crimean-Congo Hemorrhagic Fever Viruses in Sivas Province, TürkiyeJ Med VirolJune 22, 2026Qianying Lin, Nazif Elaldi, Mesut Yigit, Baris Yildiz, Roger Hewson, Martín López-García, Grant Lythe, Ayse Nur Pektas, Binnur Koksal, Tuba Nur Tasseten, et al.Diagnostics, Türkiye). Illumina sequencing libraries were prepared using the Nextera XT v2 kit (Cat. No. FC‐131‐1096, Illumina) and sequenced on a 2 × 150 bp paired‐end Illumina MiSeq platform operated by Gen‐Era Diagnostics.

(3) Protocol for simultaneous profiling of transcription start sites and full-length transcripts from low-input samples using Smart-seq+5′STAR ProtocolsJune 19, 2026Diego Rodriguez-Terrones, Marlies E. Oomen, Maria-Elena Torres-Padillacommercial assays ,, Kapa HiFi ReadyMix,Roche,KM2605 Nextera XT DNA Library preparation kit,Illumina,FC-131-1096 Qubit dsDNA HS Assay Kit,ThermoFisher Scientific,Q32854 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, dT30V

(4) Whole genome sequence of Tsukamurella tyrosinosolvens extracted from metagenome of human pleural fluid enriched in Mycobacteria Growth Indicator TubeMicrobiology Resource AnnouncementsJune 9, 2026Rameez Moidu Jameela, Mayur Mahindra Kedare, Rehbar Khan, Nehal Dhankad, Rishav Kumar Sinha, Amrutraj Zade, Sanchi Shah, Anirvan Chatterjeeprepared from this extracted DNA using the Nextera XT DNA Library Preparation Kit (Illumina Cat. No. FC-131-1096) and sequenced using DNBSEQ-T7 machine from MGI as per manufacturer’s protocol. Quality of such generated

(5) Integration of Transcriptional Signatures from Brain Tissue and Plasma Extracellular Vesicles of a Preclinical Tauopathy Mouse ModelInt J Mol SciJune 3, 2026Tanzima Tarannum Lucy, A. N. M. Mamun-Or-Rashid, Daniel C. Lee, Iliya Lefterov, Radosveta Koldamova, Nicholas Francis FitzKit (#634773, Takara Bio, San Jose, CA) combined with the Nextera XT DNA Library Preparation Kit (#FC-131-1096, Illumina Inc., San Diego, CA, USA) and Illumina DNA/RNA UD Indexes Set A (#20091654, Illumina, CA).

(6) Optimisation and validation of capture mNGS for predicting antimicrobial resistanceEBioMedicineJune 3, 2026Qiao Lin, Xu Mei, Huimin Zheng, Jie Meng, Fusheng He, Bin Yang, Xiao Ru, Mengting Su, Danlan Wang, Ni Tan, et al.(Qiagen, 50214). DNA libraries were constructed using the Nextera XT DNA Library Prep Kit (Illumina, FC-131-1096) according to the manufacturer's protocol. DNA libraries underwent probe hybridisation and enrichment

(7) Transient tumor exposure induces persistent functional defects in memory CD8+ T cellsiScienceMay 15, 2026Daphné Laubreton, Margaux Prieux, Sophia Djebali, Maxence Dubois, Simon De Bernard, Olivier Gandrillon, Christophe Arpin, Jacqueline MarvelBiosystem,Cat#KK2602 Agencourt AMPure XP,Beckman,Cat# A63880 Nextera XT DNA sample preparation kit,Illumina,Cat#FC-131-1096 Nextera XT 96-index kit,Illumina,Cat#FC-131-1002 ,, Critical commercial assays,Critical commercial

(8) Treg-derived IFN-γ supports the differentiation of Th1-Treg in tumor immunity and autoimmunityFront ImmunolMay 12, 2026Ayumi Kuratani, Masaaki Okamoto, Sekar Arumsari, Takashi Kobayashi, Miwa Sasai, Masahiro Yamamotoinstruction. An Illumina library was prepared using a Nextera DNA Library Preparation Kit (Illumina, Cat#FC-131-1096) following SMARTer kit instructions. Sequencing was performed on an Illumina NovaSeq 6000 sequencer

(9) Diverse paths to broadly neutralizing antibody escape among HIV-1 strainsNature MicrobiologyMay 11, 2026Alex C. Stabell, Songhee Lee, Debby J. Park, Christy L. Lavine, Sebastian Mejia Espinosa, Viren A. Baharani, Rachel Patejak, Michel C. Nussenzweig, Marina Caskey, Michael S. Seaman, et al.a template for Illumina-based library preparation using the Nextera XT DNA library preparation kit (FC-131-1096) and Nextera XT Index v2 Set A barcodes (FC-131-2001). Library preparation was performed to the manufacturer’s

(10) A decade of antimicrobial resistance in Vibrio spp.: genomic and functional insightsMicrobiology SpectrumMay 5, 2026Agila Kumari Pragasam, Chandana Basak, Mansi Rajwanshi, Lekshmi Narendrakumar, Goutam Chowdhury, Prabhakar Babele, Meenal Chawla, Orlando Moranchel, Upma Singh, Deboleena Roy, et al.samples involved library preparation steps using the Illumina Nextera XT library preparation kit (#FC-131-1096). Samples requiring a minimum quantity of 1 ng, as measured with the Qubit Flex Fluorometer, underwent

(11) Enhancing inference of differential gene expression in metatranscriptomes from human microbial communitiesNature CommunicationsApril 21, 2026Evan M. Lee, Nathan P. McNulty, Matthew C. Hibberd, Jiye Cheng, Kazi Ahsan, Hao-Wei Chang, Barak A. Cohen, Jeffrey I. GordonDNA libraries were generated from raw DNA using the Nextera XT DNA Library Preparation Kit (Illumina; FC-131-1096). Barcoded Complementary DNA (cDNA) libraries were constructed from raw RNA using the Stranded Total

(12) Impact of Oral Pre-Exposure Secretory IgA Prophylactic Produced in Rice on Gut Microbiome HomeostasisPharmaceuticsApril 9, 2026Ravi Bharadwaj, Carlos Gaspar, Tyler D. Moeller, Doyle Ward, Mark S. Klempner, Yang Wang, Lisa A. Cavaciniand metagenomic DNA sequencing libraries were constructed using the Nextera XT DNA Library Prep Kit (FC-131-1096, Illumina, San Diego, CA, USA) and sequenced on a NextSeq2000 Sequencing System as 2 × 150 nucleotide

(13) Comparative analysis of flavivirus sfRNA dynamics and secondary structurebioRxivApril 8, 2026Camden R. Bair, David VanInsberghe, Anice C. Lowen, Graeme L. Conn, Christopher J. Neufeldtstaining solution. Libraries were constructed with the Nextera XT DNA Library Preparation Kit (Illumina, FC-1311096) and Illumina DNA/RNA UD Indexes with 1 ng cDNA (tagmented) as input. The final amplified libraries

(14) An integrative single-nucleus multiomic atlas of the human left ventricle identifies gene regulatory network dynamics across cardiac development, aging, and diseaseGenome BiologyApril 6, 2026William Gao, Peng Hu, Brittney Wick, Qi Qiu, Hongjie Zhang, Ying Li, Xiangjin Kang, Kenneth Bedi, Maximilian Haeussler, Kotaro Sasaki, et al.with 10 μL of water. We then tagmented cDNA using the Nextera XT DNA sample preparation kit (Illumina, FC-131–1096), starting with 550 pg of cDNA pooled in equal amounts, from all PCR reactions for a given run. Following

(15) Caenorhabditis elegans populations shape their microbial environmentnpj Biofilms and MicrobiomesApril 4, 2026Rahul Bodkhe, Kris Sankaran, Michael ShapiraPCR reactions with primers 515 F and 806 R and with Illumina Nextera XT DNA Library Preparation Kit (Cat. #FC-131-1096) to generate sequencing amplicon libraries of the bacterial 16S V4 region, as previously described28.

(16) Peptide signaling in the paraventricular thalamus contributes to disrupted adult reward behaviors after early-life adversitybioRxivApril 2, 2026Amalia Floriou-Servou, Ryan Weber, Yuncai Chen, Cassandra L. Kooiker, Madison R. Tetzlaff, Matthew T. Birnie, Annabel K. Short, Roshae Roberts, Heidi Y. Liang, Magdalena Gantuz, et al.conversion, amplification and library preparation with Nextera XT DNA Library Preparation Kit (Illumina FC-131-1096). The RNA and DNA concentrations were measured with the Qubit™ 1X RNA High Sensitivity (HS) Broad Range

(17) ECHOS enables spatial epigenome profiling at subcellular resolutionbioRxivMarch 31, 2026Qiqi Cao, Qianlan Xu, Yusuke Ueda, Shreya Rajachandran, Manjita Sharma, Xin Zhang, Mala Mahendroo, Edward J. Grow, Haiqi ChencDNA was used to generate sequencing libraries using an Illumina Nextera XT Library Preparation kit (FC-131–1096). CUT&Tag The HeLa cell CUT&Tag experiment was performed using the CUT&Tag-IT Core Assay Kit (Active

(18) Metagenome Sequencing and Recovery of 52 Microbial Genomes from Plastic-Polluted Coastal SedimentScientific DataMarch 30, 2026Arunmozhi Bharathi Achudhan, Rajnish Narayanan, Thirumurthy Madhavanapplications. Whole-genome libraries were then prepared using the Nextera XT Library Preparation Kit (FC-131-1096), and library quality, including fragment size distribution, was assessed using the QIAxcel Connect

(19) DNA-dependent RNA polymerase Incorporates β-D-N4-hydroxycytidine (NHC) Linking Molnupiravir to Host Transcription-Dependent MutagenesisJournal of Biological ChemistryMarch 26, 2026Nathalie Garnier, Hongtao Xu, Raymond F. Schinazi, Baek Kimafter running 1% agarose gel. DNA libraries were prepared with Nextera XT DNA library Prep (Illumina, FC-131-1096) for 1 ng DNA input; tagmentation, amplifying and clean up library. Their quality was verified by Bioanalyser

(20) Human-associated NDM-5-producing multidrug-resistant Escherichia coli detected in retail beef and pork in Hungary, 2021Frontiers in BioinformaticsMarch 24, 2026Mirena Ivanova, Joana Mourão, Judit Szarvas, Elif S. Tosun, Niamh Lacy-Roberts, Natasia Rebekka Thornval, Zita Záborcki, Szilárd Jánosi, Raquel Garcia-Fierro, Pierre-Alexandre Beloeil, et al.instructions. Sequencing libraries were constructed using the Nextera XT Library Prep Kit (Cat. No. FC-131-1096, Illumina, San Diego, CA, United States), loaded on a NextSeq 500/550 Mid Output v2.5 Kit (300 cycles)

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Nextera XT DNA Library Preparation Kit (96 Samples) from Illumina

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Nextera XT DNA Library Preparation Kit (96 Samples) from Illumina

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