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Assay Kits
Assay Kit
Nextera XT DNA Library Preparation Kit (96 Samples)
Nextera XT DNA Library Preparation Kit (96 Samples) from Illumina
Be the first to write a review!
Citations:
(803)
Supplier Page
Supplier Page from
Illumina for
Nextera XT DNA Library Preparation Kit (96 Samples)
Description
90-minute tagmentation-based library prep for small genomes, PCR amplicons, plasmids, and cDNA; high-throughput format
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Nextera XT DNA Library Preparation Kit (96 Samples)
Company
Illumina
Price
Supplier Page
View Company Product Page
Catalog Number
FC-131-1096
Applications
Amplicon sequencing, WGS, De novo sequencing, Metagenomics
Reactivity
Any
Type
DNA Library Prep Kit
Quantity
96 Samples
Add to Compare List
Illumina
5200 Illumina Way
San Diego
,
CA
92122
United States
Phone
: 858.202.4500
Company Profile
Website:
http://illumina.com
Citations (803)
(1)
Children’s gut microbiota predicts the efficacy of obesity treatment
Gut Microbes
December 31, 2026
Mireia Alcázar, Verónica Luque, Natalia Ferré, Judit Muñoz-Hernando, Mariona Gispert-Llauradó, Ricardo Closa-Monasterolo, Albert Feliu, Gemma Castillejo, Joaquín Escribano
was used to initiate the protocol. The
multiplexing
step was performed with a Nextera XT Index Kit (
FC-131-1096
) (
Illumina
, San Diego, CA, USA). One μl of the
PCR product
was run on a Bioanalyzer DNA 1000 chip to
(2)
Stimulation of Adult Muscle Stem Cells with BMPs Results in Direct Activation of Notch Pathway Genes
Cells
August 19, 2026
Birthe Katrin Alexandra Lange, Ioanna Polydorou, Viktoriia Huryn, Angelina M. Georgieva, Shanshan You, Thomas Müller, Susanne Morales-Gonzalez, Bettina Brandt, Carmen Birchmeier, Helge Amthor,
et al.
preamplification of the cDNA [59] and continued with the Nextera XT
DNA library prep
kit (
Illumina
,
FC-131-1096
) using the MGI universal and index primers for
PCR amplification
. The libraries were quality-checked
(3)
Comparative analysis of flavivirus sfRNA dynamics and secondary structure
Journal of Virology
August 18, 2026
Camden R. Bair, David VanInsberghe, Anice C. Lowen, Graeme L. Conn, Christopher J. Neufeldt
staining solution. Libraries were constructed with the Nextera XT
DNA Library Preparation
Kit (
Illumina
,
FC-131-1096
) and Illumina DNA/RNA UD Indexes with 1 ng cDNA (tagmented) as input. The final amplified libraries
(4)
Influence of heterotrophs on phage infection of marine picocyanobacteria
mSystems
August 18, 2026
Allison Coe, Sierra M. Parker, Nhi N. Vo, Sean M. Kearney, Shaul Pollak, Konnor von Emster, James I. Mullet, Kurt G. Castro, Sallie W. Chisholm
described in Coe et al. (39).
Sequencing
libraries were prepared with the Illumina NexteraXT kit (cat#
FC-131-1096
,
Illumina
, San Diego, CA) following the manufacturer’s protocol. Samples were indexed using the TG NexteraXT
(5)
Parkinson’s disease-associated PINK1 loss disrupts ensheathing glia and causes dopaminergic neuron synapse loss
Elife
August 13, 2026
Lorenzo Ghezzi, Sabine Kuenen, Ulrike Pech, Nils Schoovaerts, Ayse Kilic, Suresh Poovathingal, Kristofer Davie, Jochen Lamote, Roman Praschberger, Patrik Verstreken
,
KAPA HiFi HotStart ReadyMix
,
Roche
,Cat#
07958927001
, "Commercial
assay
, kit",Nextera XT
DNA Library Preparation
Kit,
Illumina
,Cat#
FC-131-1096
, "Peptide, recombinant protein",Dispase I,Sigma-Aldrich,Cat#D4818, "Peptide, recombinant protein",Collagenase
(6)
E3 Ubiquitin Ligase Mind Bomb 1 is a Novel Negative Regulator of Tumor-Suppressive BMP Signaling
Cancer Res Commun
August 1, 2026
Charisa L. Cottonham, Gloria E. Hernandez, Tommy K. Cheung, Christopher M. Rose, Rajini Srinivasan, Robert Piskol, Xiaosai Yao, Christian W. Siebel
cDNA were used to make
sequencing
libraries using Nextera XT DNA Sample Preparation Kit (
Illumina
, #
FC-131-1096
). For regular input RNA, the
sequencing
library was generated using the Truseq Stranded mRNA Kit (
Illumina
,
(7)
SARS-CoV-2 Surveillance in Free-Ranging Wildlife in New England and Virginia, United States, 2022–2025
Viruses
July 29, 2026
Idrissa Nonmon Sanogo, Wendy B. Puryear, Alexa F. Simulynas, Elena Cox, Maureen Murray, Zain Khalil, Harm van van Bakel, Martin J. R. Feehan, Zak Mertz, Priya Patel,
et al.
verified by
gel electrophoresis
. Next, we performed library preparation using the Nextera XT kit (cat.
FC-131-1096
,
Illumina
, San Diego, CA, USA) followed by paired-end
sequencing
(2 × 150 nt) on the Illumina MiSeq
(8)
Accurate, sensitive, and efficient chromatin accessibility quantification at target loci using UNIChro-seq
Nature Communications
July 20, 2026
Michihiro Kono, Hiroaki Hatano, Kenichiro Asahara, Masahiro Nakano, Reza Bagherzadeh, Tsugumi Kawashima, Takahiro Arakawa, Miho Sato, Hajime Inokuchi, Takahiro Nishino,
et al.
were prepared with SMART-seq mRNA (Takara Bio, 634773) and Nextera XT
DNA Library Prep
Kit (
Illumina
,
FC-131-1096
). We mapped the sequence reads to the reference genome by STAR69 (v2.7.9a) and quantified the reads
(9)
Rapid clinical validation of an RNA/DNA hybrid tagmentation-based metagenomic workflow for respiratory RNA virus detection
Front Microbiol
July 15, 2026
Xia Ma, Shan Guo, Yangyang Feng, Murong Su, Feili Wei, Xuejun Liu
performed using an optimized protocol adapted from the Nextera XT
DNA Library Prep
Kit (
Illumina
, Cat. No.
FC-131-1096
). Compared with the previously described CATCH workflow, optimization focused on consolidating enzymatic
(10)
The maternal KRAB-ZFP ZFPOBI1 reveals structural constraints governing ERV transcriptional co-option in mouse oocytes
Front Cell Dev Biol
July 14, 2026
Andrina J. Stäubli, Yan Huang, Louise J. H. Rasmussen, Freja Mosegaard Hansen, Hui Mun Loh, Michael J. Claxton, Daniel M. Messerschmidt
performed on 1 ng of cDNA per reaction using the Illumina Nextera XT DNA sample preparation kit (
Illumina
,
FC-131-1096
).
NGS
libraries were amplified for 10 cycles, pooled and sequenced Illumina NextSeq2000 using 100 bp
(11)
Evaluating myxovirus resistance protein A-based rapid testing combined with pathogen sequencing for arboviral and incidental viral infection surveillance in Senegal
Microbiology Spectrum
July 7, 2026
Mouhamed Kane, Serge Freddy Moukaha Doukanda, Safietou Sankhé, Bocar Sow, Marie Henriette Dior Ndione, Moundhir Mhamadi, Madeleine Dieng, Seynabou Mbaye Ba Souna Diop, Seynabou Seye, Maimouna Mbanne,
et al.
sequencing
libraries were prepared using the Nextera XT
DNA Library Preparation
Kit (
Illumina
; Cat#
FC-131-1096
). Paired-end
sequencing
(2 × 150 bp) was carried out on an Illumina MiSeq platform using standard run
(12)
Macrophage-specific targeting of histone demethylases with small-molecule inhibitors suppresses inflammatory response in vivo
J Biol Chem
July 6, 2026
Niranjana Natarajan, Thaybah Ahmed, Balaji Govindaswamy, Devika S. Manickam, Jishnu Das, Kabirul Islam, Partha Dutta
with full length cDNA were advanced to library preparation using Illumina Nextera XT kit (
Illumina
:
FC-131-1096
) with 12
PCR
cycles. Libraries were pooled and sequenced on an Illumina NextSeq 500 using a High Output
(13)
CD38 defines a therapeutically targetable pathogenic T cell population for precision immunotherapy in autoimmune diabetes
bioRxiv
July 3, 2026
Shiva Pathak, Rizwan Ahmed, Nadine Nagy, Sooyeon Lee, Cameron S. Bader, Shobha Regmi, Bettina P. Iliopoulou, Pin-I Chen, Biki Gupta, Alejandro Villar-Prados,
et al.
Qubit. Libraries were constructed using the Nextera XT
DNA Library Preparation
Kit (
Illumina
, Cat.
FC-131-1096
) and indexed via tagmentation and
PCR amplification
to generate Illumina-compatible libraries. Indexed
(14)
Isolation and Characterization of ΦCA1NRNZ, a Lytic Bacteriophage Targeting the Emerging Device-Associated Pathogen Cutibacterium avidum
Antibiotics (Basel)
July 3, 2026
Ron Braunstein, Amit Rimon, Roni Teitelbaum, Suhnit Coppenhagen-Glazer, Vered Molho-Pessach, Ronen Hazan
sequencing
.
Sequencing
libraries were constructed using the Nextera XT
DNA Library Preparation
kit (
FC-131-1096
,
Illumina Inc
., San Diego, CA, USA) according to the manufacturer’s protocol, with reagent and input
(15)
An atlas of primate insular cortex reveals a signal-processing strategy in von Economo neurons
Nature Cell Biology
July 2, 2026
Rui-Feng Liu, Mengyao Huang, Yuhui Shen, Mingting Shao, Junzhan Jing, Nana Xu, Lei Tang, Biaodi Liu, Jianming Shi, Fanrui Chen,
et al.
quality control. We constructed the cDNA libraries using Nextera XT
DNA Library Preparation
(
Illumina
,
FC-131-1096
) and Nextera XT Index kit V2 Sets A (Illumina, FC-131-2001) following the manufacturer’s instructions.
(16)
Single-bacterium RNA-seq protocol to uncover heterogeneous expression of coding and noncoding genes in Bacteroides thetaiotaomicron
STAR Protoc
July 1, 2026
Elise Bornet, Alexander J. Westermann
store,KK2602/SKU:7958935001 MEGAshortscript kit,
ThermoFischer
Scientific,
AM1354
Monarch
RNA cleanup
kit,
NEB
,
T2050S
Nextera XT kit,
Illumina
,
FC-131-1096
Qubit dsDNA HS assay kit
,
Invitrogen
,
Q32854
TE buffer
,
Invitrogen
,
AM9849
Oligo Clean & Concentrator
,
Zymo Research
(17)
Multimodal characterization of variation in neuronal types in the mouse basal ganglia
bioRxiv
June 29, 2026
Agata Budzillo, Matt Mallory, Rachel Dalley, Changkyu Lee, Xuehan Ci, Xiao-Ping Liu, Sarah Walling-Bell, Rusty Mann, Nelson Johansen, Ellie Adams,
et al.
in the SMART-Seq v4 kit. All samples proceeded through Nextera XT
DNA Library Preparation
(
Illumina
FC-131-1096
) using either Nextera XT Index Kit V2 Set A-D (FC-131-2001,2002,2003,2004) or custom dual-indexes provided
(18)
The E‐cadherin‐Wnt‐mir‐994 Axis Repurposes a Cadherin Switch for Niche Robustness and Germline Stem Cell Maintenance
Cell Proliferation
June 26, 2026
Renjun Tu, Hiu Laam Yau, Runzhi Deng, Sarah E. Webb, Shiyuan Chen, Jianquan Ni, Ting Xie
(634888; Takara Bio Inc.) with subsequent library preparation by Nextera XT DNA Sample Preparation kit (
FC‐131‐1096
;
Illumina
) and Index kits (FC‐131‐1002; Illumina). cDNA samples and libraries were both confirmed on
(19)
Type I Interferon-Driven Monocyte Dysregulation and MAS-associated CD8 + T cells During Macrophage Activation Syndrome
bioRxiv
June 25, 2026
Susan P. Canny, Hannah A. DeBerg, Emma L. Kuan, Nicholas Moss, Griffin Gessay, Ailing Lu, Amanda Huang, Allison R. O’Rourke, Erik D. Layton, Paige Bouvatte,
et al.
for
Sequencing
(Takara Bio USA, Cat #634891) and Nextera XT
DNA Library Preparation
Kit (
Illumina
,
FC-131–1096
). Libraries were pooled, quantified by a
Qubit Fluorometer
(
Life Technologies
) and sequenced on a NextSeq
(20)
Monocytic niches escape T cell surveillance and promote Mycobacterium tuberculosis persistence in lymph nodes
Immunity
June 23, 2026
Elya A. Shamskhou, Fergal J. Duffy, Lauren M. Cross, Courtney R. Plumlee, Benjamin H. Gern, Holly W. Barrett, Alan H. Diercks, Sara B. Cohen, Ramya Sivakumar, John D. Aitchison,
et al.
v4 Ultra Low Input RNA Kit,Takara Bio,Cat# 88-8824-00 Nextera XT
DNA Library Prep
Kit,
Illumina
,Cat#
FC-131-1096
LymphoLyte,
Cedarlane
,Cat#
CL5035
Deposited data,Deposited data,Deposited data
Mycobacterium tuberculosis
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Nextera XT DNA Library Preparation Kit (96 Samples) from Illumina
Supplier Page
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