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Nextera XT DNA Library Preparation Kit (24 Samples)
Nextera XT DNA Library Preparation Kit (24 Samples) from Illumina
Be the first to write a review!
Citations:
(562)
Supplier Page
Supplier Page from
Illumina for
Nextera XT DNA Library Preparation Kit (24 Samples)
Description
90-minute tagmentation-based library prep for small genomes, PCR amplicons, plasmids, and cDNA with low DNA input requirement
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Nextera XT DNA Library Preparation Kit (24 Samples)
Company
Illumina
Price
Supplier Page
View Company Product Page
Catalog Number
FC-131-1024
Applications
Amplicon sequencing, WGS, De novo sequencing, Metagenomics
Reactivity
Any
Type
DNA Library Prep Kit
Quantity
24 Samples
Add to Compare List
Illumina
5200 Illumina Way
San Diego
,
CA
92122
United States
Phone
: 858.202.4500
Company Profile
Website:
http://illumina.com
Citations (562)
(1)
Phenotypic and whole genome characterization of multidrug-resistant Campylobacter coli from chicken liver
Poultry Science
October 1, 2026
Mª Pilar González-Navarro, Alicia Manzanares-Pedrosa, Florencia Correa-Fiz, Lauge Holm Sørensen, Teresa Ayats, Núria Aloy, Miquel Nofrarías, Rene S. Hendriksen, Marta Cerdà-Cuéllar
(Illumina HiSeq). The libraries were prepared with Nextera XT DNA sample preparation kit (cat. no.
FC-131-1024
;
Illumina, Inc
., San Diego, CA, USA) followed by multiplexed paired-end
sequencing
with a read length
(2)
ATR activity regulates DNA replication and RNA polymerase II transcription during S-phase
iScience
August 21, 2026
Jianming Wang, Sudipta Pathak, Megan Jones, Jingwen Mao, Marco Saponaro
Blotting Substrate,Sigma-Aldrich,Cat#DUO92008 SuperScript III Reverse Transcriptase kit,
Illumina
,Cat#
FC-131-1024
,, Deposited data,Deposited data,Deposited data ,, RNAPII
ChIP-Seq
data,This paper,GEO: GSE269242 BrdU-Seq
(3)
Isotype-specific antibody secreting plasma cells harbor epigenetic and transcription factor architectures defining distinct cell lineages
J Immunol
August 17, 2026
Lou-Ella M M George-Alexander, Roshni Roy, Sakeenah L Hicks, Mansi Gupta, Christopher D Scharer, Jeremy M Boss
libraries were generated using 200 pg of complementary DNA as input for the NexteraXT kit (
Illumina
;
FC-131-1024
) with 12 cycles of
polymerase chain reaction (PCR
)
amplification
. Final sequence libraries were quantitated
(4)
Axonal defasciculation is restricted to specific branching points during regeneration of the lateral line nerve in zebrafish
Development
August 15, 2026
Rohan S. Roy, A. J. Hudspeth
of cDNA was then used to prepare libraries using the Illumina Nextera XT DNA sample preparation kit (
FC-131-1024
,
Illumina
). Libraries generated from hair cells 1 day post nerve lesion and control hair cell samples
(5)
Divergent C. elegans toxin alleles are suppressed by distinct mechanisms
Elife
August 11, 2026
Stefan Zdraljevic, Laura Walter-McNeill, Giancarlo N Bruni, Joshua S Bloom, JB Collins, Daniel HW Leighton, Heriberto Marquez, Noah Alexander, Leonid Kruglyak
generation 10, DNA libraries were generated using the Illumina Nextera XT
DNA Library Preparation
Kit (#
FC-131-1024
) and whole genome sequenced using
Illumina
NextSeq2000 P1 reagents (20074933). For generation 4, allele
(6)
Genome-wide mapping of DCP2-dependent 5' cap footprints in Arabidopsis thaliana
NAR Genom Bioinform
August 10, 2026
Neha Shukla, Michael A Schon, Vivek K Raxwal, Michael D Nodine, Karel Riha
cDNA was tagmented, and
library amplification
was performed using NextraXT kit (
Illumina
, Catalog No.
FC-131-1024
) following manufacturer’s protocol. The final libraries were checked on a fragment analyzer and subsequently
(7)
Microglial clonal dynamics and the impact of clonal hematopoiesis in autologously transplanted rhesus macaques
Cell Reports
July 28, 2026
Yifan Zhou, Diana M. Abraham, Rohan V. Hosuru, Taha Bartu Hayal, Ashley L. Gin, Xing Fan, Byung-Chul Lee, Taehoon Shin, E. Lake Potter, So Gun Hong,
et al.
V4 Ultra Low Input RNA Kit,Clontech,Cat#634889 Nextera XT
DNA Library Preparation
Kit,
Illumina
,Cat#
FC-131-1024
Nextera XT Index Kit,Illumina,Cat#FC-131-1001 Neural Tissue Digestion Kit,
Miltenyi Biotec
,Cat#
130-094-802
Percoll®,VWR
(8)
Dose-dependent supplementation of Schizochytrium in the biofloc system modulates dual microbiomes to enhance growth and survival in Pacific white shrimp (Litopenaeus vannamei)
Front Microbiol
July 22, 2026
Zhi Li, Xiaohan Li, Buwen Sun, Hongwei Wu, Jingyi Zhang, Xiulin Wan, Baohua Zhao, Nasi Xiao, Zhen Qi, Qingyang Li,
et al.
concentrations, and approximately 5 μg
sequencing
libraries were created using a TruSeq DNA Sample Prep Kit (
FC-131-1024
,
Illumina
, United States) before being sequenced on an Illumina PE300 platform (Illumina, United States). Raw
(9)
NodoMap: A single-cell and spatial transcriptomic atlas of the mouse nodose ganglion
Cell Press Blue
July 20, 2026
Sijing Cheng, Georgina K.C. Dowsett, Kara Rainbow, Mariana Norton, Anna G. Roberts, Phyllis Phuah, Gavin A. Bewick, Brian Y.H. Lam, Giles S.H. Yeo, Kevin G. Murphy
kit,Curio Bioscience,"SQ8002, K001, K002, K003" Nextera XT
DNA Library Preparation
Kit,"
Illumina
, USA",
FC-131-1024
,, Deposited data,Deposited data,Deposited data ,, NodoMap - snRNASeq of
mouse
nodose
ganglion
,This
(10)
Gut commensal bifidobacteria are associated with restrained inflammatory reprogramming in human monocyte-derived dendritic cells
iScience
July 17, 2026
Sayaka Ishihara, Tatsuki Nishimura, Riko Mishima, Eri Mitsuyama, Akira Sen, Hiroki Kaneko, Yukihiro Hishida, Miyuki Tanaka, Toshitaka Odamaki
RAW2,Macherey-Nagel,Cat#740359.80 SMART-Seq mRNA LP,Takara Bio,Cat#634768 Nextera XT
DNA Library Prep
Kit,
Illumina
,Cat#
FC-131-1024
"IDT for Illumina - DNA/RNA UD Indexes, Tagmentation",
Illumina
,Cat#20091654
NovaSeq X
Series 25B Reagent
(11)
Decoding stage-specific functions of PRC2 in early embryogenesis uncovers roles in preimplantation development and primordial germ cell fate
Nat Cell Biol
July 10, 2026
Chengjie Zhou, Meng Wang, Zhiyuan Chen, Yi Zhang
634890) was used to construct cDNA libraries, and Nextera® XT DNA Sample Preparation Kit kit (
Illumina
,
FC-131–1024
) was used for the sequence libraries construction. All libraries were sequenced by the Illumina NextSeq
(12)
TREM1 signaling amplifies neutrophil-mediated inflammation in inflammatory bowel disease
Nature Communications
July 8, 2026
Warrison Andrade, Hao Xu, Yutian Peng, Zhiyuan Yao, Jack Bevers, Rhyan Puno, Yongchang Shi, Victoria Pham, Meena Choi, Yonglian Sun,
et al.
were used to make
sequencing
libraries with the Nextera XT DNA Sample Preparation Kit (
Illumina
, cat. #
FC‑131‑1024
). Libraries were quantified with the
Qubit dsDNA HS Assay Kit
(
Thermo Fisher
, cat. #
Q32852
) and the
(13)
Cerebellar microRNA-206 tunes Purkinje neuron firing dynamics to control sensorimotor gating
bioRxiv
June 30, 2026
Mary P. Heyer, Masago Ishikawa, Junshi Wang, John Erol Evangelista, Amanda K. Fakira, Avi Ma'ayan, Guoping Feng, Paul J. Kenny
Illumina,
New England Biolabs
,Cat#
E7560S
Nextera XT DNA Sample Preparation Kit for 24 Samples,
Illumina
,Cat#
FC-131-1024
Curio Seeker 3×3 Seeker Kit Bundle V1.1 (8 tiles),Curio Biosciences,Cat#SK004 Curio Seeker Dual Indexing
(14)
Epidemiological and Epizootological Monitoring and Spatiotemporal Dynamics of Plague in Natural Foci of Kazakhstan
Pathogens
June 29, 2026
Ziyat Abdel, Zauresh Zhumadilova, Raikhan Mussagalieva, Aigul Abdirassilova, Svetlana Issaeva, Galina Kovaleva, Bolatbek Baitursyn, Beck Abdeliyev, Temirkhan Sagidulin, Nurbol Shaki,
et al.
MD, USA). DNA libraries were prepared using the Nextera XT
DNA Library Preparation
Kit (catalog No.
FC-131-1024
) according to the manufacturer’s protocol.
Sequencing
was performed on the
Illumina
MiSeq platform using
(15)
Local and systemic immune correlates of anal high-risk HPV infection and clearance in men living with HIV and men at high risk for HIV
Frontiers in Microbiology
June 25, 2026
Veronica Ober, Eva Gruener, Danni Wang, Olga Baranov, Renate Stirner, Gabriele Reiling, Raffaele Conca, Christopher Dächert, Gerardo Ibarra, Ulrich Seybold,
et al.
(
Roche
; #7958935001)]. Libraries were generated with Nextera XT
DNA Library Preparation
Kit (
Illumina
; #
FC-131-1024
) and Nextera XT Index Kit v2 Set (Illumina; FC-131-2001) according to manufacturer’s protocol but using
(16)
Linking Composition and Function: A Two-Year Metatranscriptomic Study of Microbial Adaptation in an Industrial Wastewater Treatment Plant
FEMS Microbiology Ecology
June 18, 2026
Asala Mahajna, Ranko Gacesa, Karel J Keesman, Gert-Jan W Euverink, Andreas Froemelt, Carlos J Melián, Bayu Jayawardhana
subsequently prepared using the Nextera XT DNA Sample Preparation Kit (
Illumina
, San Diego, CA, USA; Cat. No.
FC-131-1024
), and
sequencing
was performed on the MiSeq platform at the University Medical Center Groningen
Sequencing
(17)
Differentiation defects reposition sebaceous glands as inflammatory instigators in the early pathogenesis of hidradenitis suppurativa
Front Immunol
June 8, 2026
Xiang Chen, Jiaqi Li, Yibo Feng, Zhanyan Pan, Qiong Wu, Xinyi Xu, Qian Zhao, Tingting Hu, Guangjie Chen, Christos C. Zouboulis,
et al.
transcription of RNA were processed. The cDNA was amplified with the Nextera XT DNA sample Pre-Kit (
FC-131-1024
,
Illumina
, San Diego, USA) to gain
sequencing
libraries. Libraries of individual samples were measured
(18)
Clinical severity-dependent virulence of Staphylococcus aureus from human diabetic foot ulcers drives impaired wound healing in a diabetic murine model
Front Cell Infect Microbiol
June 5, 2026
Keun Seok Seo, Lee M. Nicols, Cara Dale Palmer, K. Taylor Hellmann, D. Ashley Robinson, Alicia K. Olivier, Justin A. Thornton, Khadija Ferdous, Wilson Cooper Brookshire, Joo Youn Park
Scientific, USA), and 150bp paired-end libraries were prepared with a Nextera XT
DNA Library Preparation
Kit (
FC-131-1024
,
Illumina
, USA).
Sequencing
was performed at the Molecular and Genomics Core Facility of the University
(19)
Human iPSCs derived from cryopreserved testicular somatic cells enable germline regeneration in childhood cancer survivors
Human Reproduction Open
June 3, 2026
Tiago Macedo, Claudia De Guidi, Leah Nic Aodha, Nageswara Rao Boggavarapu, Maja Piechocka, Xuan Ye, Francesca Mastropasqua, Victoria Keros, Ulrika Norén Nyström, Per Frisk,
et al.
and tagmentation of DNA were performed using the Nextera XT kit (
Illumina Inc
., San Diego, CA, USA, #
FC-131-1024
) along with IDT® for Illumina® DNA Unique Dual Index barcodes. The final amplified libraries were purified
(20)
Commensal-derived acetylcholine enhances mucosal immune education
Nature
June 3, 2026
Deguang Song, Brianna Duncan-Lowey, Varnica Khetrapal, Randy Hamchand, Tong Deng, Hailey Brown, Anchi Wu, Anjelica L. Martin, Kaylyn M. Bauer, Yanyu Zhao,
et al.
Sequencing
libraries were prepared using the Illumina Nextera XT library preparation kit (
Illumina
,
FC-131-1024
) and barcoded using the Nextera XT Index Kit V2 (Illumina, FC−131-2001). The libraries were sequenced
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Nextera XT DNA Library Preparation Kit (24 Samples) from Illumina
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