Description
Set of reagents and buffers tailored the straightforward labeling of biomolecules with d2, as part of the development of time-resolved fluorescence resonance energy transfer (TR-FRET) assays. The d2 labeling kit is suitable for quick, easy labeling of peptides, proteins, and oligonucleotides with d2.This kit is designed for very small-volume fast labeling needs, such as determining the best antibody among several antibodies available for HTRF assay development.The N-hydroxysuccinimide activated d2 supplied reacts with primary amines carried by the biomolecules to label, for simplified conjugation.The kit includes all the materials necessary for the labeling procedure (except dialysis and concentration devices).HTRF assays offer many advantages over other technologies:
Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays