Description
Description: This Human Retinol-binding protein 3 (RBP3) ELISA Kit employs a two-site sandwich ELISA to quantitate RBP3 in samples. An antibody specific for RBP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RBP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Retinol-binding protein 3, interstitial (RBP3), also known as IRBP is a protein identified in most eutherians except tenrecs and armadillos.The interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process. The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da