Description
Intended Uses: This PRLR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat PRLR. This ELISA kit for research use only!
Principle of the Assay: PRLR ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-PRLR antibody and an PRLR-HRP conjugate. The assay sample and buffer are incubated together with PRLR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRLR concentration since PRLR from samples and PRLR-HRP conjugate compete for the anti-PRLR antibody binding site. Since the number of sites is limited, as more sites are occupied by PRLR from the sample, fewer sites are left to bind PRLR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRLR concentration in each sample is interpolated from this standard curve