Description
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to RNPEP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to RNPEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNPEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RNPEP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RNPEP in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids