Description
Intended Uses: LH ELISA Kit is intended for the quantitative measurement of LH in human serum or plasma.
Principle of the Assay: The LH ELISA kit is a solid phase assay using streptavidin/biotin method. The samples and Anti-LH/AntiBiotin conjugate are added to the wells coated with Streptavidin. LH in the patient's serum forms a sandwich between specific antibodies labeled with biotin and HRP. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of LH in the samples. A standard curve is prepared relating color intensity to the concentration of the LH