Description
Background: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of sST2 in human serum, plasma, cell culture supernatants and other biological fluids.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for human sST2 has been pre-coated onto a microplate.Standards and samples are pipetted into the wells and any sST2 present is bound by the immobilized antibody. After washing away any unbound substances, and then a detection antibody specific for sST2 is added to the wells and binds to the combination of capture antibody sST2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate solution is added to the wells and color develops in proportion to the amount of sST2 bound in the initial step. The color development is stopped and the absorbance is measured