Pyruvate Kinase Activity Assay Kit (Colorimetric) MA-PK from RayBiotech

Product Specs
ItemPyruvate Kinase Activity Assay Kit (Colorimetric)
CompanyRayBiotech
Price $419.00Supplier Page View Company Product Page
Catalog NumberMA-PK
Detection TargetPyruvate Kinase
Quantity1 Plate Kit, 2 Plate Kit, 5 Plate Kit
ApplicationsMetabolism Assays
Research AreaCancer, Energy Metabolism, Glycolysis & Lipid Metabolism
Sample TypePlasma, Serum, Tissue Lysates, Cell Lysates, Saliva, Urine
Sensitivity0.67 U/L

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RayBiotech

3607 Parkway Lane, Ste. 200

Peachtree Corners, Georgia 30092

United States

Phone: 770-729-2992
888-494-8555

Company Profile

Website: www.RayBiotech.com

(21) The role of miR-155-5p in inflammation and mechanical loading during intervertebral disc degenerationCell communication and signaling : CCSAugust 28, 2024Cazzanelli P, Lamoca M, Hasler J, Hausmann ON, Mesfin A, Puvanesarajah V, Hitzl W, Wuertz-Kozak K.non-targeting control conditions. Phosphorylation array The phosphorylation of proteins associated with the MAPK pathway was determined with the Human/Mouse MAPK Phosphorylation Array (RayBiotech Life, AAH-MAPK-1–8).

(22) Mitomycin C and its analog trigger cytotoxicity in MCF-7 and K562 cancer cells through the regulation of RAS and MAPK/ERK pathwaysChemico-Biological InteractionsMay 25, 2024Owen Zacarias, Cristina C. Clement, Shu-Yuan Cheng, Melissa Rosas, Christina Gonzalez, Marion Peter, Peter Coopman, Elise Champeillines treated with MC and DMC The semi-quantitative detection of phosphorylated human proteins in the MAPK/ERK and JAK/STAT signaling pathways in the cell lysates from the untreated (control) and MC, DMC treated

(23) Metabolic self-feeding in HBV-associated hepatocarcinoma centered on feedback between circulation lipids and the cellular MAPK/mTOR axisCell Communication and SignalingMay 21, 2024Ying Zhu, Yingke Zhao, Zhouyu Ning, Yong Deng, Bing Li, Yun Sun, Zhiqiang Mengsupernatant containing the soluble protein fraction was collected. To assess the protein expression of the MAPK/AKT/mTOR signaling pathway, a Human phosphorylation array C1 (RayBio/AAH-AKT-1-8) was utilized. The

(24) Activation of MEK-ERK-c-MYC signaling pathway promotes splenic M2-like macrophage polarization to inhibit PHcH-liver cirrhosisFrontiers in immunologyJanuary 1, 2024Guihu W, Wei D, Hailong Z, Chongyu Z, Jin S, Mengchen Z, Jian Z, Rui Z, Song R, Chen Z, et al.listed in Supporting Supplementary Table S3 . 2.10 Protein phosphorylation array Phosphorylation of MAPK signaling proteins (p-CREB, p-ERK1/2, p-GSK3a, p-GSK3b, p-MEK, and p-MSK2) was detected using a human/mouse

(25) Effects of Metformin on JNK Signaling Pathway and PD-L1 Expression in Triple Negative Breast CancerCancer management and researchJanuary 1, 2024Wang R, Zhao Y.20,210,841) was purchased from Sino-American Shanghai Bristol-Myers Squibb Pharmaceutical Co., LTD. MAPK pathway phosphorylation array kit (item number: AAH-MAPK-1-2) was purchased from RayBiotech (USA). Fetal

(26) Mfap4: a promising target for enhanced liver regeneration and chronic liver disease treatmentNPJ Regenerative medicineNovember 7, 2023Iakovleva V, Wuestefeld A, Ong ABL, Gao R, Kaya NA, Lee MY, Zhai W, Tam WL, Dan YY, Wuestefeld T.arrays Whole-cell protein extracts were prepared from mouse tissue samples, and the protein array was done using MAPK pathway phosphorylation Array (RayBiotech, C1; cat# AAH-MAPK-1-8). The procedures were done according

(27) Phytoceramide Alleviates the Carrageenan/Kaolin-Induced Arthritic Symptoms by Modulation of InflammationBiomolecules & therapeuticsSeptember 1, 2023Sur B, Kim M, Villa T, Oh S.and stimulated with IL-1β (10 ng/mL) for 24 h. Supernatants were analyzed for TNF-α, IL-6, PGE2, and MAPK (RayBiotech Inc., GA, USA) with the use of ELISA kits as described earlier. Statistical analysis Statistical

(28) Identification of ligand-receptor pairs that drive human astrocyte developmentNature neuroscienceAugust 1, 2023Voss AJ, Lanjewar SN, Sampson MM, King A, Hill EJ, Sing A, Sojka C, Bhatia TN, Spangle JM, Sloan SA.phosphorylation events across five major signal transduction pathways (BMP/TGFβ, AKT/mTOR, JAK/STAT, RAS/MAPK, and NF-κB) (Figure 7A–E). We exposed hCOs to the gliogenic cocktail for 30 days (day 60–90) and then

(29) Thiophene derivative inflicts cytotoxicity via an intrinsic apoptotic pathway on human acute lymphoblastic leukemia cellsPloS oneJanuary 1, 2023Swain RM, Sanchez A, Gutierrez DA, Varela-Ramirez A, Aguilera RJ.measurements, and 10,000 events per sample were acquired (Gallios; Kaluza software; Beckman Coulter). MAPK and JAK-STAT array To gage the phosphorylation of essential biological pathways sometimes responsible

(30) Discovery of caffeoylisocitric acid as a Keap1-dependent Nrf2 activator and its effects in mesangial cells under high glucoseJournal of enzyme inhibition and medicinal chemistryDecember 1, 2022Yao H, Zhang W, Yang F, Ai F, Du D, Li Y.kits were provided by Colorfulgene Biotechnology (Wuhan, China). ELISA kits for activated members of MAPK family including phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2), phosphorylated

(31) Salvianolic acid B inhibits autophagy and activation of hepatic stellate cells induced by TGF-β1 by downregulating the MAPK pathwayFront PharmacolAugust 4, 2022Na Jiang, Jing Zhang, Jian Ping, Lieming Xudeath by activating the JNK pathway. Previous research had shown autophagy can be regulated by the MAPK signaling pathway (Hanyu et al., 2020) (Chiou et al., 2020). In order to determine the signal pathway

(32) Verbascoside potentiates the effect of tyrosine kinase inhibitors on the induction of apoptosis and oxidative stress via the Abl-mediated MAPK signalling pathway in chronic myeloid leukaemiaExperimental and therapeutic medicineAugust 1, 2022Akgun-Cagliyan G, Cort-Donmez A, Kilic-Toprak E, Altintas F.for 10 days. The colonies were photographed every 5 days (Olympus CKX41). Cell-based ELISA measuring MAPK protein expression levels Total/phosphorylated protein levels of ERK, p38 and JNK were determined using

(33) Endothelial ACKR3 drives atherosclerosis by promoting immune cell adhesion to vascular endotheliumBasic research in cardiologyJune 8, 2022Gencer S, Döring Y, Jansen Y, Bayasgalan S, Yan Y, Bianchini M, Cimen I, Müller M, Peters LJF, Megens RTA, et al.protocol. Phosphorylation array Silenced and control cell culture samples were analyzed for the modifications in the MAPK pathway with the commercially available Human/Mouse MAPK Phosphorylation Array kit (AAH-MAPK-1-8) from

(34) Lysine 63-linked ubiquitination of tau oligomers contributes to the pathogenesis of Alzheimer's diseaseThe Journal of biological chemistryApril 1, 2022Puangmalai N, Sengupta U, Bhatt N, Gaikwad S, Montalbano M, Bhuyan A, Garcia S, McAllen S, Sonawane M, Jerez C, et al.phosphorylation profile array. Bar graphs present protein fold-change of signaling activity involved in (A) MAPK, (B) Akt, (C) JAK/STAT, (D) NFκB, and (E) TGFβ pathways. Statistical analysis was calculated by One-way

(35) Somatostatin Receptor Splicing Variant sst5TMD4 Overexpression in Glioblastoma Is Associated with Poor Survival, Increased Aggressiveness Features, and Somatostatin Analogs ResistanceInternational journal of molecular sciencesJanuary 20, 2022Fuentes-Fayos AC, G-García ME, Pérez-Gómez JM, Peel A, Blanco-Acevedo C, Solivera J, Ibáñez-Costa A, Gahete MD, Castaño JP, Luque RM.analyze the differential phosphorylation levels of 55 proteins belonging to 5 key oncogenic pathways (MAPK, AKT, JAK/STAT, NF-κB, and TGF-β) in response to sst5TMD4 overexpression, following the manufacturer’s

(36) Metformin Inducing the Change of Functional and Exhausted Phenotypic Tumor-Infiltrated Lymphocytes and the Correlation with JNK Signal Pathway in Triple-Negative Breast CancerBreast cancer (Dove Medical Press)January 1, 2022Wang R, Li Y, Zhao Y, Shi F, Zhou Q, Wu J, Lyu S, Song Q.for 15min, and then the absorbance was measured at 570nm. The experiment was repeated three times. MAPK Pathway Analysis The effect of metformin on MAPK signaling pathway in 4T1 cells was determined by protein microarray

(37) Luteolin delays photoreceptor degeneration in a mouse model of retinitis pigmentosaNeural regeneration researchOctober 1, 2021Liu XB, Liu F, Liang YY, Yin G, Zhang HJ, Mi XS, Zhang ZJ, So KF, Li A, Xu Y.Expression levels of phosphorylated proteins were detected by Human/Mouse Mitogen-Activated Protein Kinase (MAPK) and Protein Kinase B (PKB/AKT) Pathway Phosphorylation Arrays (RayBiotech, Peachtree Corners, GA, USA),

(38) Inflammatory conversion of quiescent osteoblasts by metastatic breast cancer cells through pERK1/2 aggravates cancer-induced bone destructionBone researchSeptember 29, 2021Back J, Nguyen MN, Li L, Lee S, Lee I, Chen F, Gillinov L, Chung YH, Alder KD, Kwon HK, et al.investigate activated kinases in osteoblastic MCF7 cells and osteolytic MDA231 cells. Two different MAPK phosphorylation assay kits were purchased from Raybiotech (Peachtree Corners, GA, USA) (cat no: AAH-MAPK-1–2)

(39) Comparative Cytotoxic Activity of Hydroxytyrosol and Its Semisynthetic Lipophilic Derivatives in Prostate Cancer CellsAntioxidants (Basel, Switzerland)August 25, 2021León-González AJ, Sáez-Martínez P, Jiménez-Vacas JM, Herrero-Aguayo V, Montero-Hidalgo AJ, Gómez-Gómez E, Madrona A, Castaño JP, Espartero JL, Gahete MD, et al.membranes for the semi-quantitative detection of 55 phosphorylated human proteins belonging to the MAPK, AKT, JAK/STAT, NFκB, and TGFβ signaling pathways were incubated for 30 min with blocking buffer at

(40) Equilibrative nucleoside transporter 1 inhibition rescues energy dysfunction and pathology in a model of tauopathyActa neuropathologica communicationsJune 22, 2021Chang CP, Chang YG, Chuang PY, Nguyen TNA, Wu KC, Chou FY, Cheng SJ, Chen HM, Jin LW, Carvalho K, et al.mice We next examined the phosphorylation level of 17 kinases and signaling molecules by using the MAPK Phosphorylation Array (RayBiotech, GA, USA). Only minor or no change was found between Tau22 mice and WT

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Pyruvate Kinase Activity Assay Kit (Colorimetric)

Pyruvate Kinase Activity Assay Kit (Colorimetric) from RayBiotech

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RayBiotech

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Pyruvate Kinase Activity Assay Kit (Colorimetric) from RayBiotech

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