Pyruvate Kinase Activity Assay Kit (Colorimetric) MA-PK from RayBiotech

Product Specs
ItemPyruvate Kinase Activity Assay Kit (Colorimetric)
CompanyRayBiotech
Price $419.00Supplier Page View Company Product Page
Catalog NumberMA-PK
Detection TargetPyruvate Kinase
Quantity1 Plate Kit, 2 Plate Kit, 5 Plate Kit
ApplicationsMetabolism Assays
Research AreaCancer, Energy Metabolism, Glycolysis & Lipid Metabolism
Sample TypePlasma, Serum, Tissue Lysates, Cell Lysates, Saliva, Urine
Sensitivity0.67 U/L

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RayBiotech

3607 Parkway Lane, Ste. 200

Peachtree Corners, Georgia 30092

United States

Phone: 770-729-2992
888-494-8555

Company Profile

Website: www.RayBiotech.com

(1) Persistent loss of membrane-associated MUC4 reprograms physiological functions in corneal epithelial cells via MAPK-dependent cascadeScientific ReportsMay 27, 2026Tsan-Chi Chen, Wan-Lin Wu, Shu-Wen ChangCorners, GA) following manufacturer’s instructions with minor modifications. The array profiles included MAPK, AKT (protein kinase B), JAK (Janus kinases)/STAT (signal transducer and activator of transcription),

(2) Hibernating bear serum triggers an anti-fibrotic signature in human fibroblasts, involving ECM remodeling and MAPK signaling activationScientific ReportsMarch 21, 2026Jade Sutter, Alexandre Geffroy, Amandine Moretton, Anne Randi Græsli, Jonas Kindberg, Lydie Combaret, Etienne Lefai, Isabelle Garreau-Balandier, Fabrice Bertile, Patrick Vernetall LFQ values obtained from the repeated analysis of the reference sample. Protein extraction and MAPK phosphorylation analysis Phosphorylation levels of key components of the MAPK pathway were assessed

(3) CCL8-dependent recruitment of natural killer cells enhances the antitumor activity of neoadjuvant chemotherapy in gastric cancerCancer immunology, immunotherapy : CIIMarch 17, 2026Wang Y, Gao P, Peng X, Wang Z, Wu M, Hao Z, Chen L, Qian Y, Wang B, Feng D, et al.h, GC cells were collected, and total protein was extracted. For mitogen-activated protein kinase (MAPK) inhibition, cells were initially treated with their respective inhibitors for 24 h separately (SP600125:

(4) Tissue-specific immune and MAPK signatures in models of reduced Progranulin and Western dietNeurobiology of DiseaseMarch 1, 2026Andrea R. Merchak, Maria Elizabeth de Sousa de Sousa Rodrigues, Cassandra Cole, Noelle Neighbarger, Nilay Bhavasar, Rebecca L. Wallings, Valerie Joers, Jianjun Chang, Sean D. Kelly, Timothy R. Sampson, et al.cortices from each group were rapidly microdissected and prepared per RayBio C-Series Human and Mouse MAPK Pathway Phosphorylation Array C1manufacturer’s (AAH-MAPK-1–4) instructions. Relative signal was determined

(5) Basal activation of ERK1/2 blunts the antimicrobial activity of neutrophils from old hosts against antibody-opsonized Streptococcus pneumoniaeJournal of Leukocyte BiologyFebruary 9, 2026Shaunna R. Simmons, Annabel Rivera, Elsa N. Bou Bou Ghanemnaïve or HI immune sera at MOI 2 for 5 min at 37 °C. PMNs lysates were saved at −80 °C and analyzed by MAPK phosphorylation array (RayBiotech) per the manufacturer’s protocol. Chemiluminescence of blots was measured

(6) Aberrant maintenance of developmental transcription factor PAX6 promotes neuronal cell death via JNK3 signalingCell Death & DiseaseJanuary 29, 2026Ji-Young Kim, Mi-Jin An, Jinho Kim, Chul-Hong Kim, Yuna Park, Geun-Seup Shin, Hyun-Min Lee, Ah-Ra Jo, Mi Jin Kim, Yujeong Hwangbo, et al.antibody array Immunoprecipitates obtained using an anti-PAX6 antibody from mouse retinal lysates were analyzed with a commercial MAPK phosphorylation antibody array (RayBiotech, Norcross, GA, USA) according to the manufacturer’s instructions.

(7) Fibrillarin Contributes to the Oncogenic Characteristics of Colorectal Cancer Cells and Reduces Sensitivity to 5-FluorouracilCancers (Basel)December 5, 2025Ting Wu, Mounira Chalabi-Dchar, Wei Xiong, Lucie Arnould, Eliezer Aimontche, Sabine Beaumel, Charles Dumontet, Virginie Marcel, Tanguy Fenouil, Jean-Jacques Diaz, et al.SW-480 shNS and shFBL cells were identified and quantitatively analyzed using the RayBio® C-Series Human MAPK Pathway Phosphorylation Array C1 (Norcross, GA, USA). Total protein extracts from shNS and shFBL cells

(8) Genetic and epigenetic screens in primary human T cells link candidate causal autoimmune variants to T cell networksNature GeneticsSeptember 18, 2025Ching-Huang Ho, Maxwell A. Dippel, Meghan S. McQuade, LeAnn P. Nguyen, Arpit Mishra, Stephan Pribitzer, Samantha Hardy, Harshpreet Chandok, Florence M. Chardon, Troy A. McDiarmid, et al.and phosphatase inhibitors and frozen at −80 °C. Then, 20 µg of lysate was incubated on pre-blocked MAPK phospho arrays (RayBiotech, AAH-MAPK-1) overnight at 4 °C. Samples were washed with diluted wash buffer

(9) The Role of c-Jun Signaling in Cytidine Analog-Induced Cell Death in MelanomaACS OmegaJuly 29, 2025Shayne Sensenbach, Han G. Ngo, Sreyashi Ghosh, Prashant Karki, Vahideh Angardi, Mehmet A. Orman(Peachtree Corners, GA). Arrays were purchased for the following signaling pathways: AKT, JAK/STAT, MAPK, NF-κB, and TGF-β (RayBiotech catalog #’s AAH-AKT-1, AAH-JAKSTAT-1, AAH-MAPK-1, AAH-NFKB-1, and AAH-TGFB-1,

(10) Loss of dynamin 1-like protein impairs mitochondrial function and self-renewal, and activates the integrated stress response in human embryonic stem cellsFrontiers in GeneticsJuly 28, 2025Artur Cieslar-Pobuda, Safak Caglayanthe relative levels of phosphorylation of 55 proteins in 5 well-known signaling pathways level of: MAPK, AKT, JAK/STAT, NF-κB, and TGFβ. Cells were lysed in Lysis Buffer supplemented with Phosphatase and

(11) Reduced somatosensory innervation alters the skeletal transcriptome at a single cell level in a mouse model of type 2 diabetesBone ResJuly 4, 2025Masnsen Cherief, Mario Gomez-Salazar, Minjung Kang, Seungyong Lee, Sowmya Ramesh, Qizhi Qin, Mingxin Xu, Soohyun Kim, Mary Archer, Manyu Zhu, et al.and 72 h of culture with or without neural CM treatment, based on the manufacturer’s protocol.98,99 A MAPK phospho-protein array was performed using C-Series Human/Mouse MAPK Phosphorylation Antibody Array kit

(12) Assessing the diagnostic, prognostic, and therapeutic potential of the somatostatin/cortistatin system in glioblastomaCell Mol Life SciApril 23, 2025Miguel E. G-García, Ana S. De la Rosa-Herencia, Álvaro Flores-Martínez, María Ortega-Bellido, Rafael Sánchez-Sánchez, Cristóbal Blanco-Acevedo, Manuel D. Gahete, Juan Solivera, Raúl M. Luque, Antonio C. Fuentes-Fayosanalyse the differential phosphorylation levels of 55 proteins belonging to 5 key oncogenic pathways (MAPK, AKT, JAK/STAT, NF-κB, and TGF-β) in response to octreotide and pasireotide [pool of n = 3 independent

(13) REG3A secreted by peritumoral acinar cells enhances pancreatic ductal adenocarcinoma progression via activation of EGFR signalingCell communication and signaling : CCSFebruary 18, 2025Ren X, Teng Y, Xie K, He X, Chen G, Zhang K, Liao Q, Zhang J, Zhou X, Zhu Y, et al.(#111-035-003, Jackson ImmunoResearch Labs, West Grove, PA, US). For the phospho-kinase array, a commercial MAPK pathway phosphorylation array (#AAH-MAPK-1-4, RayBiotech, Norcross, GA, US) was used following the manufacturer’s

(14) Cellular and Molecular Evidence of the Synergistic Antitumour Effects of Hydroxytyrosol and Metformin in Prostate CancerInternational journal of molecular sciencesFebruary 5, 2025Porcel-Pastrana F, Montero-Hidalgo AJ, G-García ME, Gil-Duque I, Prats-Escribano A, Gahete MD, Sarmento-Cabral A, Luque RM, León-González AJ.membranes for the semi-quantitative detection of 55 phosphorylated human proteins belonging to the MAPK, AKT, JAK/STAT, NF-κB, and TGF-β signalling pathways were incubated for 30 min with blocking buffer

(15) PS-MPs Induced Inflammation and Phosphorylation of Inflammatory Signalling Pathways in LiverToxicsDecember 22, 2024Mengchao Ying, Naimin Shao, Cheng Dong, Yijie Sha, Chen Li, Xinyu Hong, Yu Ding, Jing Xu, Kelei Qian, Gonghua Tao, et al.Peachtree Corners, GA, USA) was used to detect the phosphorylation levels of proteins involved in the MAPK, AKT, JAK/STAT, NF-κB, and TGF-β signalling pathways. The prechilled lysis buffer was used to extract

(16) TNO155 is a selective SHP2 inhibitor to target PTPN11-dependent oral squamous cell carcinomaHeliyonNovember 15, 2024Chai AWY, Tan YH, Ooi S, Yee PS, Yee SM, Cheong SC.used to analyse the differential phosphorylation levels of 55 proteins in five oncogenic pathways (MAPK, AKT, JAK/STAT, NF-κB, and TGF-β) in response to TNO155 treatment. Lysates used were OSCC cells treated

(17) Synthetic Antiangiogenic Vascular Endothelial Growth Factor-A Splice Variant Revascularizes Ischemic Muscle in Peripheral Artery DiseaseJournal of the American Heart AssociationOctober 15, 2024Raja A, Ganta V.VEGF165bKD→RR for 10 minutes using Human Phosphorylation Pathway Profiling Array C55 consisting of JAK–STAT, MAPK, AKT, TGF‐β, and NF‐kB signaling mediators (Cat No: AAH‐PPP‐1‐8, RayBiotech). Briefly, HUVECs were cultured

(18) Zinc transporter ZnT5 is associated with epithelial mesenchymal transition via SMAD1 in breast cancerInternational journal of experimental pathologyOctober 1, 2024Iwabuchi E, Miki Y, Xu J, Kanai A, Ishida T, Sasano H, Suzuki T.(Peachtree Corners, GA); this array can detect 55 phosphorylated human proteins in five signalling pathways: MAPK, AKT, JAK/STAT, NFκB and TGF‐β. MCF‐7 cells were transfected with 5 nM ZnT5‐specific siRNA (Santa Cruz

(19) High gestational leucine level dampens WDPCP/MAPK signaling to impair the EMT and migration of cardiac microvascular endothelial cells in congenital heart defectsPulmonary circulationOctober 1, 2024Hong W, You G, Luo Z, Zhang M, Chen J.quantity of each factor was determined using the serially diluted standards provided in each kit. Phospho‐MAPK and phospho‐ERK measurement Phospho‐p38 MAPK and phospho‐ERK levels were measured using the Human/Mouse/Rat Phospho‐ERK1/2, JNK, p38 MAPK Cell‐Based ELISA kit

(20) Analysis of potential TAK1/Map3k7 phosphorylation targets in hypertrophy and cachexia models of skeletal muscleBiology openSeptember 15, 2024Nasehi F, Rylance C, Schnell E, Greene MA, Conway C, Hough Z, Duckett S, Muise-Helmericks RC, Foley AC.cells further stimulated with NE (TAK1OE+ NE) (Fig. 1) using the RayBiotech C1-Series Human and Mouse MAPK pathway phosphorylation array. All kinases with changes in the phosphorylation status greater than the

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Pyruvate Kinase Activity Assay Kit (Colorimetric)

Pyruvate Kinase Activity Assay Kit (Colorimetric) from RayBiotech

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Pyruvate Kinase Activity Assay Kit (Colorimetric) from RayBiotech

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