hTERT Immortalized RPTEC Growth Kit ACS-4007 from ATCC

Product Specs
ItemhTERT Immortalized RPTEC Growth Kit
CompanyATCC
Price Supplier Page View Company Product Page
Catalog NumberACS-4007
TypeMedia Sera Reagents
ApplicationsAdd to DMEM:F12 (ATCC® 30-2006™) to produce complete medium for hTERT Immortalized RPTEC cells (ATCC® CRL-4031™)

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) The Cardiac Circadian Clock Regulates Rhythms in Peripheral Tissues via Fibulin 5Compr PhysiolApril 10, 2026Sharanya S. Bettadapura, David C. Tangeman, Sushumna B. Satyanarayana, Madison M. Ruhmann, Willa J. Bonds, J. Harrison Edwards, Pooja Gupta, Aykhan Yusifov, William D. Todd, Danielle R. Brunsthe base medium containing DMEM: F12 Medium (ATCC 30–2006) and the RPTEC Growth Kit components (ATCC ACS-4007) consisting of 5 pM triiodo-L-thyronine, 10 ng/mL recombinant human EGF, 3.5 μg/mL ascorbic acid, 5.0

(2) A VPS33B CRISPR knockout study: In vitro evidence of an adhesion defectPLoS OneFebruary 13, 2026Maria Caluianu, Kimberley A. Owenwere cultured in DMEM:F12 Medium (ATCC® 30–2006™), supplemented with hTERT RPTEC Growth Kit (ATCC® ACS-4007™) (1% Supplement A and 1.6% Supplement B), 2% FBS, and 0.1 mg/mL Geneticin™ Selective Antibiotic (G418

(3) An in vitro-in silico workflow for predicting renal clearance of environmental chemicals and drugsToxicologyJanuary 1, 2026Courtney Sakolish, Hsing-Chieh Lin, Haley L. Moyer, Lucie C. Ford, Charles H. Christen, Barbara A. Wetmore, Michael J. DeVito, Philip Hewitt, Stephen S. Ferguson, Farah Raad, et al.the vendor in DMEM/F12 (#30–2006, ATCC) supplemented with the “hTERT Immortalized RPTEC Growth Kit” (#ACS-4007, ATCC), with a final concentration of 0.1 mg/mL Geneticin™ Selective Antibiotic (G418 Sulfate; Gibco,

(4) Impact of C-Terminal PKC Phosphorylation on TRPC6 Current KineticsInternational Journal of Molecular SciencesNovember 27, 2025Maximilian Keck, Sebastian Pöll, Hannah Schmelzer, Tabea Kressmann, Christian Hermann, Michael Mederos y Schnitzler, Ursula StorchDMEM:F12 Medium (ATCC; Cat. No. 30-2006) combined with hTERT Immortalized RPTEC Growth Kit (ATCC; Cat. No. ACS-4007). All cells were held at 37 °C in a humified atmosphere with 5% CO2. HEK293T cells were transfected

(5) Regulation of Klotho Production by Mineralocorticoid Receptor Signaling in Renal Cell LinesBiomoleculesOctober 25, 2025Elena Kohm, Martina Feger, Michael FöllerL-glutamine and 15 mM HEPES (Gibco) supplemented with the hTERT immortalized RPTEC growth kit (ATCC-ACS-4007, ATCC), G418 solution (G418-RO, Sigma-Aldrich, Schnelldorf, Germany), 100 U/mL penicillin, and 100 μg/mL

(6) Evaluating Ion Mobility Data Acquisition, Calibration, and Processing for Small Molecules: A Cross-Platform Assessment of Drift Tube and Traveling Wave MethodologiesJournal of the American Society for Mass SpectrometryJuly 2, 2025James N. Dodds, Lucie C. Ford, Jack P. Ryan, Amie M. Solosky, Ivan Rusyn, Erin S. BakerCell culture medium consisted of DMEM F-12 (cat. no. 30–2006, ATCC), hTERT Immortalized RPTEC Growth Kit (cat. no. ACS-4007, ATCC), and G-418 Solution (G418-RO, Roche, Basel, Switzerland). Cells were cultured in 384-well plates

(7) Protocol to develop a proximal tubule-on-chip model based on hiPSC-derived kidney organoids for functional analysis of renal transportersSTAR ProtocolsJune 20, 2025Cheng Ma, Ramin Banan Sadeghian, Ryosuke Negoro, Kazuya Fujimoto, Toshikazu Araoka, Naoki Ishiguro, Minoru Takasato, Ryuji Yokokawasolution,Nacalai,35553 Paraformaldehyde,Sigma-Aldrich,158127 Triton X-10,Sigma-Aldrich,T9284-500ML hTERT immortalized RPTEC growth kit,ATCC,ACS-4007 "REGM, BulletKit, REBM plus SingleQuots of growth supplements",Lonza,CC-3190 G-418 solution,Sigma-Aldrich,108321-42-2 Donkey serum

(8) TFE3 fusions drive oxidative metabolism and ferroptosis resistance in translocation renal cell carcinomaEMBO Molecular MedicineMarch 27, 2025Alexandra Helleux, Guillaume Davidson, Antonin Lallement, Fatima Al Hourani, Alexandre Haller, Isabelle Michel, Anas Fadloun, Christelle Thibault-Carpentier, Xiaoping Su, Véronique Lindner, et al.supplemented with 2.5 mM L-Glutamine, 15 mM HEPES, hTERT Immortalized RPTEC growth kit-supplement A&B (ATCC ACS-4007), gentamycin (40 µg/mL), and G418 (100 µg/mL). 786-O ccRCC cells were grown in RPMI 1640 medium supplemented

(9) Comparative Analysis of Proximal Tubule Cell Sources for In Vitro Studies of Renal Proximal Tubule ToxicityBiomedicinesFebruary 24, 2025Courtney Sakolish, Han-Hsuan D. Tsai, Hsing-Chieh Lin, Piyush Bajaj, Remi Villenave, Stephen S. Ferguson, Jason P. Stanko, Richard A. Becker, Philip Hewitt, Weihsueh A. Chiu, et al.were cultured in DMEM:F12 (#30-2006; ATCC) supplemented with the hTERT-immortalized RPTEC growth kit (#ACS-4007; ATCC) and a G418 supplement (final concentration of 0.1 mg/mL; Geneticin G418 Sulfate, Gibco). Cryopreserved

(10) SETD2 loss-of-function uniquely sensitizes cells to epigenetic targeting of NSD1-directed H3K36 methylationGenome BiolFebruary 5, 2025Ryan T. Wagner, Ryan A. Hlady, Xiaoyu Pan, Liguo Wang, Sungho Kim, Xia Zhao, Louis Y. El Khoury, Shafiq Shaikh, Jian Zhong, Jeong-Heon Lee, et al.#11,140–050), sodium pyruvate (ThermoFisher, catalog #11,360–070), RPTEC growth kit (ATCC, catalog #ACS-4007), and 250 μg/mL G418 (ThermoFisher, catalog #10,131–035) for maintaining selection of hTERT expression.

(11) Proximal tubule-on-chip as a model for predicting cation transport and drug transporter dynamicsSci RepJanuary 20, 2025Isy Petit, Quentin Faucher, Jean-Sébastien Bernard, Perrine Giunchi, Antoine Humeau, François-Ludovic Sauvage, Pierre Marquet, Nicolas Védrenne, Florent Di Meo(ATCC, reference: 30–2006) supplemented with hTERT immortalized RPTEC Growth kit (ATCC, reference: ACS-4007), penicillin/streptomycin (Gibco, reference: 15,140,122) at 1% and Geneticin (Gibco, reference: 10,131,035)

(12) Evaluating nephrotoxicity reduction in a novel polymyxin B formulation: insights from a 3D kidney-on-a-chip modelAntimicrobial agents and chemotherapyOctober 8, 2024Payasi A, Yadav MK, Chaudhary S, Aggarwal A.sourced: Dulbecco's Modified Eagle's Medium (DMEM):F12 medium (ATCC 30-2006), hTERT RPTEC growth kit (ATCC ACS-4007), trypsin-EDTA for primary cells (ATCC PCS-999-003), and soybean trypsin inhibitor (ATCC 30–2104) from

(13) Decreased PANK1 expression in kidney renal clear cell carcinoma: impact on cell apoptosis, invasion, migration, and epithelial-mesenchymal transitionDiscover OncologyAugust 28, 2024Xiang Liu, Song Gao, Ye-Min Qin, Wei-Li Zhang, Peng Li, Xiao-Yun Xiangcells were maintained in DMEM: F12 medium (30-2006, ATCC) supplemented with an hTERT RPTEC growth kit (ACS-4007, ATCC). HK-2 cells were cultured in keratinocyte serum-free medium (K-SFM, 17005-042, ATCC). The cells

(14) An in vitro-in silico workflow for predicting renal clearance of PFASToxicology and Applied PharmacologyAugust 1, 2024Hsing-Chieh Lin, Courtney Sakolish, Haley L. Moyer, Paul L. Carmichael, Maria T. Baltazar, Stephen S. Ferguson, Jason P. Stanko, Philip Hewitt, Ivan Rusyn, Weihsueh A. Chiucultured in DMEM:F12 (Cat# 30-2006, ATCC) supplemented with the hTERT Immortalized RPTEC Growth Kit (Cat# ACS-4007, ATCC), and a final concentration of 0.1 mg/mL G418 (Geneticin™ G418 Sulfate; Cat# 10131035, Gibco,

(15) A Cell Pre‐Wrapping Seeding Technique for Hydrogel‐Based Tubular Organ‐On‐A‐ChipAdvanced ScienceAugust 1, 2024Jing Nie, Sha Lou, Andreas M. A. O. Pollet, Manon van Vegchel, Carlijn V. C. Bouten, Jaap M. J. den Toonder(ATCC‐30‐2006, LGC Standards GmbH, Wesel, DE) supplemented with hTERT Immortalized RPTEC Growth Kit (ATCC‐ACS‐4007, LGC Standards GmbH, Wesel, DE), 10% fetal bovine serum (FBS) (16 140 071, CAS Number: 64742‐49‐0, Fisher

(16) SETD2 loss in renal epithelial cells drives epithelial-to-mesenchymal transition in a TGF-β-independent mannerMolecular oncologyJanuary 1, 2024Wang T, Wagner RT, Hlady RA, Pan X, Zhao X, Kim S, Wang L, Lee JH, Luo H, Castle EP, et al.sodium pyruvate (Thermo Fisher; catalog #11360070), RPTEC growth kit (ATCC, Manassas, VA, USA; catalog #ACS‐4007) supplement A 5 mL, supplement B 8 mL, and 250 μg·mL−1 G418 (Thermo Fisher; catalog #10131035) for maintaining

(17) The essential role of the ERK activation in large T antigen of BK polyomavirus regulated cell migrationVirus researchOctober 15, 2023Wang JW, Li YJ, Wu HH, Hsu HH, Chang MY, Wang RY, Tian YC.supplemented with 1% fetal bovine serum (Gibco™ 10437028) and the hTERT immortalized RPTEC growth kit (ATCC® ACS-4007™). Once cells reach 80–90% confluence in a T75 flask, discard the culture medium, wash cells with 5

(18) Cells sorted off hiPSC-derived kidney organoids coupled with immortalized cells reliably model the proximal tubuleCommunications BiologyMay 4, 2023Ramin Banan Sadeghian, Ryohei Ueno, Yuji Takata, Akihiko Kawakami, Cheng Ma, Toshikazu Araoka, Minoru Takasato, Ryuji Yokokawalasted 5 days, DMEM/F12 (Gibco 11320033) supplemented with hTERT immortalized RPTEC growth kit (ATCC® ACS4007™) was used as culture medium per manufacturer’s instructions. The cells were trypsinized and resuspended

(19) The complex, dynamic SpliceOme of the small GTPase transcripts altered by technique, sex, genetics, tissue specificity, and RNA base editingFrontiers in Cell and Developmental BiologyNovember 17, 2022Akansha S. Das, Emily C. Sherry, Robert M. Vaughan, Marian L. Henderson, Jacob Zieba, Katie L. Uhl, Olivia Koehn, Caleb P. Bupp, Surender Rajasekaran, Xiaopeng Li, et al.6-well plates, growing cells until confluent in DMEM:F12 supplemented with RPTEC growth kit (ATCC, #ACS-4007), followed by either sheer stress (150 RPM using a MaxQ CO2 plus shaker) or static conditions for 1

(20) Multimodal single cell sequencing implicates chromatin accessibility and genetic background in diabetic kidney disease progressionNature communicationsSeptember 6, 2022Wilson PC, Muto Y, Wu H, Karihaloo A, Waikar SS, Humphreys BD.(hTERT)-immortalized human RPTEC (ATCC; CRL-4031) were cultured with ATCC hTERT Immortalized RPTEC Growth Kit (ATCC, ACS-4007). HEK293T cells (ATCC; CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco; 11965092)

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hTERT Immortalized RPTEC Growth Kit from ATCC

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hTERT Immortalized RPTEC Growth Kit from ATCC

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