Description
Enzymatic DNA fragmentation library prep designed to enable highly sensitive applications — accessing meaningful insights from a broad range of sample types, including ultra-low inputs and FFPE. It harnesses the benefits of enzymatic fragmentation, such as ease of automation, improved scalability, and preservation of low-input samples. It also reduces artifact formation, including false chimeric reads and hairpin artifacts, which can convolute variant calling in challenging applications such as translational oncology.
- Up to a 90% reduction in sequence artifacts improves assay accuracy — critical for highly sensitive applications such as low-frequency variant calling
- Highly tunable fragmentation delivers consistent library sizes over a wide input range (<1 ng to 500 ng) and between library batches
- Enzymatic fragmentation improves sequencing economy and accuracy with FFPE samples
- Built with automation in mind, including minimum transfer volumes and generous reagent overages to address automation dead volume requirements
- Streamlined workflow delivers PCR-free libraries in under 90 minutes and scales easily for high-throughput processing
- High-efficiency workflow enables PCR-free prep with lower input amounts