Description
This experiment aims to enumerate B cells that secrete SARS-CoV-2 receptor binding domain (RBD) specific IgG antibodies. It is also possible to count all cells that secrete IgG (total IgG).
The kit includes a pre-coated monoclonal anti-human IgG antibody, recombinant RBD-WASP (i.e. recombinant RBD with site-specific peptide tag WASP (PDYRPYDWASPDYRD)), biotinylation detection monoclonal antibody, anti-WASP-alp, ELISpot plate with Streptavidin-ALP, polyclonal activator and ready-made BCIP/NBT-plus substrate. The pre-coated plate reduces the measurement time and minimizes variability