Fig 1: C1 and C2 combinations modulate the dysregulated TNF/IL-17/MAPK/FoxO/mTOR/Hippo/Wnt pathways in septic mice. (A–C) ELISA detection of the phosphorylation levels of core pathway molecules (p-p65, p-p38, p-STAT3, p-mTOR, p-FoxO3a, p-YAP, p-TAZ, active β-catenin) in the liver (A), lung (B), and kidney (C) tissues. (D–F) Relative mRNA expression of core pathway genes (Rela, Mapk14, Stat3, Mtor, Foxo3a, Yap1, Wwtr1, Ctnnb1) in the liver (D), lung (E), and kidney (F) tissues. G-I: Western blot analyses of YAP (~75 kDa), TAZ (~55 kDa), β-catenin (~92 kDa), and α-Tubulin (~50 kDa, loading control) protein expression in the liver (G), lung (H), and kidney (I) tissues from Sham, CLP, C1, and C2 groups. Cropped images were processed uniformly without altering the original data integrity. Statistical analysis: One-way ANOVA with Tukey’s post-hoc test (A–F); n = 5 per group; Data are presented as the mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, no significant difference.