Description
The T7 mScript™ Complete Standard mRNA Production System provides all enzymes and enzyme-related reagents for making canonical uridine-containing, 5'-capped, 3'-polyadenylated mRNA with greatly reduced double-stranded RNA content, lower than what can be achieved with special T7 RNA polymerase mutants. The kit includes modules for in vitro transcription of linear double-stranded DNA templates using the T7 mScript™ Enzyme Solution and the canonical nucleotides ATP, CTP, GTP and UTP, enzymatic capping of the RNA using the ScriptCap™ Cap 1 Capping System that contains both ScriptCap™ Capping Enzyme and 2'-O-Methyltransferase for making mRNA with a Cap 1 cap structure, A-Plus™ Poly(A) Polymerase for adding a 3'-poly(A) tail, enzymatic removal of dsRNA content using the Min-Immune™ Gold dsRNA Removal system and 5 M NH4OAc as a convenient RNA/mRNA purification method. Kit includes a limited non-exclusive, non-transferable label license to use the products purchased from CELLSCRIPT™ solely for purchaser's own internal laboratory research use.