Description
The LIVE-Step™ Cell Assay System is designed for high-throughput, homogeneous, sensitive luminescence quantification of metabolically active, viable mammalian cells. It consists of a single ready-to-use solution to lyse the cells and measure the amount of ATP present in culture in one step. Detection is linear up to >100,000 suspension cells and >50,000 adherent cells with an R2 value greater than 0.99. The signal is stable for longer than 5 hours (T1/2 >300 min) allowing for experimental flexibility. This product is compatible with multiple media formulations and the presence of phenol red, and stable after multiple freeze-thaw cycles