Description
The Epinephrine/Adrenaline (EPI) ELISA quantitates EPI in serum, plasma and other biological fluids.
Principle of the method
The Competitive multispecies ELISA research use only kit is designed to quantitatively measure The Epinephrine/Adrenaline (EPI) independent of species. A Epinephrine/Adrenaline standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve.Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Epinephrine/Adrenaline is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Epinephrine/Adrenaline in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization